Gene description for EIF4G3
Gene name eukaryotic translation initiation factor 4 gamma, 3
Gene symbol EIF4G3
Other names/aliases eIF-4G 3
eIF4G 3
eIF4GII
Species Homo sapiens
 Database cross references - EIF4G3
ExoCarta ExoCarta_8672
Vesiclepedia VP_8672
Entrez Gene 8672
HGNC 3298
MIM 603929
UniProt O43432  
 EIF4G3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for EIF4G3
Molecular Function
    RNA cap binding GO:0000339 TAS
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 ISS
    protein binding GO:0005515 IPI
    translation factor activity, RNA binding GO:0008135 TAS
Biological Process
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 NAS
    regulation of translational initiation GO:0006446 TAS
    spermatid development GO:0007286 ISS
    negative regulation of autophagy GO:0010507 IMP
    positive regulation of translation GO:0045727 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 4F complex GO:0016281 IBA
    eukaryotic translation initiation factor 4F complex GO:0016281 NAS
    intracellular membraneless organelle GO:0043232 ISS
 Experiment description of studies that identified EIF4G3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for EIF4G3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 LIG4 3981
Two-hybrid Homo sapiens
4 EXOSC7 23016
Co-fractionation Homo sapiens
5 UTRN 7402
Co-fractionation Homo sapiens
6 CCAR1 55749
Co-fractionation Homo sapiens
7 LGR4 55366
Affinity Capture-MS Homo sapiens
8 EIF3C 8663
Affinity Capture-MS Homo sapiens
9 EIF3K 27335
Affinity Capture-MS Homo sapiens
10 PSMD11 5717
Co-fractionation Homo sapiens
11 TARDBP 23435
Affinity Capture-MS Homo sapiens
12 HSPA1L 3305
Affinity Capture-MS Homo sapiens
13 AIM1L  
Affinity Capture-MS Homo sapiens
14 EIF4A1 1973
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 EIF4EBP2 1979
Affinity Capture-MS Homo sapiens
16 SOX2  
Affinity Capture-MS Homo sapiens
17 MSI1  
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 EIF4ENIF1 56478
Affinity Capture-MS Homo sapiens
20 QPRT 23475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 DDX42 11325
Co-fractionation Homo sapiens
22 DYRK1A 1859
Affinity Capture-MS Homo sapiens
23 KIF23 9493
Affinity Capture-MS Homo sapiens
24 EIF3B 8662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 HSPH1 10808
Co-fractionation Homo sapiens
26 GUSBP5  
Affinity Capture-MS Homo sapiens
27 ABI1 10006
Co-fractionation Homo sapiens
28 SF3A2 8175
Co-fractionation Homo sapiens
29 ANGEL1  
Affinity Capture-MS Homo sapiens
30 GSPT1 2935
Affinity Capture-MS Homo sapiens
31 THRSP  
Affinity Capture-MS Homo sapiens
32 EIF3E 3646
Affinity Capture-MS Homo sapiens
33 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 GOPC 57120
Co-fractionation Homo sapiens
35 DDX6 1656
Affinity Capture-MS Homo sapiens
36 KPTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MYCN  
Affinity Capture-MS Homo sapiens
38 PABPC4 8761
Co-fractionation Homo sapiens
39 PSMC3 5702
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
40 EIF2S3 1968
Affinity Capture-MS Homo sapiens
41 CAMK2A 815
Affinity Capture-MS Homo sapiens
42 BAG2 9532
Affinity Capture-MS Homo sapiens
43 USP10 9100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RPL4 6124
Affinity Capture-MS Homo sapiens
45 EIF2B5 8893
Co-fractionation Homo sapiens
46 LRRFIP2 9209
Co-fractionation Homo sapiens
47 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PABPC1 26986
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
49 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 EIF4G2 1982
Co-fractionation Homo sapiens
52 TGM1 7051
Affinity Capture-MS Homo sapiens
53 HECTD1 25831
Affinity Capture-MS Homo sapiens
54 CTR9 9646
Affinity Capture-MS Homo sapiens
55 EIF4EBP1  
Affinity Capture-MS Homo sapiens
56 NFATC2IP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 HAO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 EIF4A2 1974
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
59 LIMA1 51474
Co-fractionation Homo sapiens
60 EIF3G 8666
Affinity Capture-MS Homo sapiens
61 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 BTNL9  
Affinity Capture-MS Homo sapiens
63 EIF4G1 1981
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
64 SIRT7  
Affinity Capture-MS Homo sapiens
65 NUP43 348995
Proximity Label-MS Homo sapiens
66 EIF4E 1977
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Far Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
67 PUS7 54517
Co-fractionation Homo sapiens
68 TGM7  
Affinity Capture-MS Homo sapiens
69 RPS27L 51065
Affinity Capture-MS Homo sapiens
70 PSPC1 55269
Affinity Capture-MS Homo sapiens
71 EXOSC3 51010
Co-fractionation Homo sapiens
72 FAM46A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 VRK2 7444
Affinity Capture-MS Homo sapiens
74 OCIAD1 54940
Affinity Capture-MS Homo sapiens
75 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 EIF3D 8664
Affinity Capture-MS Homo sapiens
77 EIF3H 8667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 RPL19 6143
Affinity Capture-MS Homo sapiens
79 EIF3F 8665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 ALDH3A2 224
Affinity Capture-MS Homo sapiens
82 VAV2 7410
Two-hybrid Homo sapiens
83 AGPAT9  
Affinity Capture-MS Homo sapiens
84 MKNK1  
Two-hybrid Homo sapiens
85 EIF3I 8668
Affinity Capture-MS Homo sapiens
86 EFNB1 1947
Affinity Capture-MS Homo sapiens
87 HSPB1 3315
Two-hybrid Homo sapiens
88 STK26 51765
Co-fractionation Homo sapiens
89 MRFAP1L1  
Affinity Capture-MS Homo sapiens
90 ESR1  
Affinity Capture-MS Homo sapiens
91 RBFOX2 23543
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 PPP1R2 5504
Co-fractionation Homo sapiens
93 SHARPIN  
Affinity Capture-MS Homo sapiens
94 EIF3M 10480
Affinity Capture-MS Homo sapiens
95 PSMC4 5704
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
96 CCT8L2  
Affinity Capture-MS Homo sapiens
97 BCAT1 586
Affinity Capture-MS Homo sapiens
98 EIF3L 51386
Affinity Capture-MS Homo sapiens
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