Gene description for EPHA1
Gene name EPH receptor A1
Gene symbol EPHA1
Other names/aliases EPH
EPHT
EPHT1
Species Homo sapiens
 Database cross references - EPHA1
ExoCarta ExoCarta_2041
Vesiclepedia VP_2041
Entrez Gene 2041
HGNC 3385
MIM 179610
UniProt P21709  
 EPHA1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for EPHA1
Molecular Function
    fibronectin binding GO:0001968 IDA
    protein kinase activity GO:0004672 IDA
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IBA
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IDA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    protein kinase binding GO:0019901 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    positive regulation of cell-matrix adhesion GO:0001954 IDA
    chromatin remodeling GO:0006338 IEA
    negative regulation of protein kinase activity GO:0006469 IDA
    cell surface receptor signaling pathway GO:0007166 IDA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    positive regulation of cell population proliferation GO:0008284 IMP
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    positive regulation of cell migration GO:0030335 IMP
    negative regulation of cell migration GO:0030336 IDA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    substrate adhesion-dependent cell spreading GO:0034446 IDA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    regulation of GTPase activity GO:0043087 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of angiogenesis GO:0045766 ISS
    protein autophosphorylation GO:0046777 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    positive regulation of stress fiber assembly GO:0051496 ISS
    activation of GTPase activity GO:0090630 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IBA
 Experiment description of studies that identified EPHA1 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EPHA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4G3 8672
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SGCA  
Affinity Capture-MS Homo sapiens
3 FAM73A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MTHFR 4524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ACOT9 23597
Affinity Capture-MS Homo sapiens
6 EIF2B2 8892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CAND2 23066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MTMR14 64419
Two-hybrid Homo sapiens
9 CAND1 55832
Affinity Capture-MS Homo sapiens
10 HOOK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SLURP1  
Affinity Capture-MS Homo sapiens
12 LTN1 26046
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
14 PRKDC 5591
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DUSP14 11072
Two-hybrid Homo sapiens
16 CTDSP2 10106
Two-hybrid Homo sapiens
17 HEATR3 55027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SMG9 56006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RINT1 60561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SLC39A11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SPAG9 9043
Affinity Capture-MS Homo sapiens
22 DIAPH3 81624
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SMC2 10592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ALK 238
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 BTAF1 9044
Affinity Capture-MS Homo sapiens
26 DFNA5 1687
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 VPS16 64601
Affinity Capture-MS Homo sapiens
28 BTNL2  
Affinity Capture-MS Homo sapiens
29 TMEM106A  
Affinity Capture-MS Homo sapiens
30 ERBB2 2064
PCA Homo sapiens
31 EXOC5 10640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 KIF14 9928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 GPN3  
Affinity Capture-MS Homo sapiens
34 STAM2 10254
Co-fractionation Homo sapiens
35 AP3M1 26985
Affinity Capture-MS Homo sapiens
36 NOP9 161424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CEACAM21  
Affinity Capture-MS Homo sapiens
38 EIF2B5 8893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NCAPD3 23310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TNPO2 30000
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CKAP5 9793
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ARMC9 80210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 OPA1 4976
Affinity Capture-MS Homo sapiens
44 STK17B 9262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 XPOT 11260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 LRBA 987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 EXOC6B 23233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PBXIP1 57326
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HAUS7  
Affinity Capture-MS Homo sapiens
50 EIF2B4 8890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TNPO1 3842
Affinity Capture-MS Homo sapiens
52 CAMK1 8536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 GTPBP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PCDHA8  
Affinity Capture-MS Homo sapiens
55 PTPN11 5781
Two-hybrid Homo sapiens
56 PLAA 9373
Affinity Capture-MS Homo sapiens
57 SMG8 55181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 TNFSF8  
Affinity Capture-MS Homo sapiens
59 NCAPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 IQCB1  
Affinity Capture-MS Homo sapiens
61 CLEC4A  
Affinity Capture-MS Homo sapiens
62 MAP3K1 4214
Biochemical Activity Homo sapiens
63 ATXN10 25814
Affinity Capture-MS Homo sapiens
64 GBF1 8729
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SMG1 23049
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 HIP1R 9026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 SAAL1 113174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 XPO4 64328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SLC7A3  
Affinity Capture-MS Homo sapiens
70 NUCB1 4924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 FBXO2 26232
Affinity Capture-MS Homo sapiens
72 C1orf112  
Affinity Capture-MS Homo sapiens
73 ASPH 444
Affinity Capture-MS Homo sapiens
74 EXOC7 23265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 EIF2B3 8891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PVR 5817
Affinity Capture-MS Homo sapiens
77 NCAPG2 54892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 DYM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 FAM114A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 SLC22A18 5002
Affinity Capture-MS Homo sapiens
81 DYNC2LI1 51626
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 USP34 9736
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 KNTC1 9735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 EXOC8 149371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
86 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 TBC1D9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 TPTE  
Two-hybrid Homo sapiens
89 RRP1 8568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 TSPAN15 23555
Affinity Capture-MS Homo sapiens
91 SMURF1 57154
Biochemical Activity Homo sapiens
92 NR2F2  
Affinity Capture-MS Homo sapiens
93 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
94 ELMO2 63916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 UBE3C 9690
Affinity Capture-MS Homo sapiens
96 SFTPC  
Affinity Capture-MS Homo sapiens
97 STAG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NF1 4763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 BRICD5  
Affinity Capture-MS Homo sapiens
100 AARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 STAG2 10735
Affinity Capture-MS Homo sapiens
102 KIAA1468 57614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 WDR13 64743
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 EPHA2 1969
Affinity Capture-MS Homo sapiens
105 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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