Gene description for KNTC1
Gene name kinetochore associated 1
Gene symbol KNTC1
Other names/aliases ROD
Species Homo sapiens
 Database cross references - KNTC1
ExoCarta ExoCarta_9735
Vesiclepedia VP_9735
Entrez Gene 9735
HGNC 17255
MIM 607363
UniProt P50748  
 KNTC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for KNTC1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
Biological Process
    mitotic sister chromatid segregation GO:0000070 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IDA
    regulation of exit from mitosis GO:0007096 NAS
    cell division GO:0051301 IEA
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 NAS
    protein-containing complex assembly GO:0065003 NAS
    protein localization to kinetochore involved in kinetochore assembly GO:1903394 IBA
Subcellular Localization
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 NAS
    cytoplasm GO:0005737 IBA
    kinetochore microtubule GO:0005828 IBA
    kinetochore microtubule GO:0005828 IDA
    kinetochore microtubule GO:0005828 NAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    actin cytoskeleton GO:0015629 IDA
    RZZ complex GO:1990423 IBA
    RZZ complex GO:1990423 IDA
    RZZ complex GO:1990423 IPI
 Experiment description of studies that identified KNTC1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KNTC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT18 3875
Proximity Label-MS Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 CA14 23632
Affinity Capture-MS Homo sapiens
5 SLC15A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CYB5B 80777
Affinity Capture-MS Homo sapiens
7 EFNA4  
Affinity Capture-MS Homo sapiens
8 TMEM9 252839
Affinity Capture-MS Homo sapiens
9 GPR45  
Affinity Capture-MS Homo sapiens
10 CXCR4 7852
Affinity Capture-MS Homo sapiens
11 GPR17 2840
Affinity Capture-MS Homo sapiens
12 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 BTNL9  
Affinity Capture-MS Homo sapiens
15 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 OPALIN  
Affinity Capture-MS Homo sapiens
18 CMTM8  
Affinity Capture-MS Homo sapiens
19 MSMO1 6307
Cross-Linking-MS (XL-MS) Homo sapiens
20 INTS6 26512
Cross-Linking-MS (XL-MS) Homo sapiens
21 LPAR6  
Affinity Capture-MS Homo sapiens
22 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NPTN 27020
Affinity Capture-MS Homo sapiens
24 Zw10  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
25 PTH2R  
Affinity Capture-MS Homo sapiens
26 C11orf74  
Affinity Capture-MS Homo sapiens
27 RP2 6102
Affinity Capture-MS Homo sapiens
28 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 LRRC27  
Affinity Capture-MS Homo sapiens
30 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
31 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CUL3 8452
Affinity Capture-MS Homo sapiens
33 FPR1  
Affinity Capture-MS Homo sapiens
34 GCGR  
Affinity Capture-MS Homo sapiens
35 VHL  
Affinity Capture-MS Homo sapiens
36 SLC18A2  
Affinity Capture-MS Homo sapiens
37 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PFN1 5216
Proximity Label-MS Homo sapiens
39 METTL21B  
Affinity Capture-MS Homo sapiens
40 PIAS4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
41 FAXC  
Affinity Capture-MS Homo sapiens
42 CRYBB3  
Affinity Capture-MS Homo sapiens
43 EDNRB 1910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 OCIAD1 54940
Affinity Capture-MS Homo sapiens
45 COMTD1 118881
Affinity Capture-MS Homo sapiens
46 CD80 941
Affinity Capture-MS Homo sapiens
47 Spire2  
Affinity Capture-MS Mus musculus
48 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 ZW10 9183
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
50 C12orf49  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SNRPB 6628
Cross-Linking-MS (XL-MS) Homo sapiens
52 ACTR5 79913
Affinity Capture-MS Homo sapiens
53 C3orf18  
Affinity Capture-MS Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 DHFR 1719
Affinity Capture-MS Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 VARS 7407
Co-fractionation Homo sapiens
58 PXN 5829
Proximity Label-MS Homo sapiens
59 RFXANK  
Affinity Capture-MS Homo sapiens
60 GPR182  
Affinity Capture-MS Homo sapiens
61 TRNT1 51095
Cross-Linking-MS (XL-MS) Homo sapiens
62 OPRL1 4987
Affinity Capture-MS Homo sapiens
63 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PPP6R1 22870
Proximity Label-MS Homo sapiens
65 EFNB1 1947
Affinity Capture-MS Homo sapiens
66 LRRC25  
Affinity Capture-MS Homo sapiens
67 CEBPA  
Protein-peptide Homo sapiens
68 SLC22A4 6583
Affinity Capture-MS Homo sapiens
69 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
71 SLC2A9  
Affinity Capture-MS Homo sapiens
72 TMEM108  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 UBXN6 80700
Affinity Capture-MS Homo sapiens
74 ITFG3 83986
Affinity Capture-MS Homo sapiens
75 MLNR  
Affinity Capture-MS Homo sapiens
76 HYPM  
Affinity Capture-MS Homo sapiens
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