Gene description for MSMO1
Gene name methylsterol monooxygenase 1
Gene symbol MSMO1
Other names/aliases DESP4
ERG25
SC4MOL
Species Homo sapiens
 Database cross references - MSMO1
ExoCarta ExoCarta_6307
Vesiclepedia VP_6307
Entrez Gene 6307
HGNC 10545
MIM 607545
UniProt Q15800  
 MSMO1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for MSMO1
Molecular Function
    C-4 methylsterol oxidase activity GO:0000254 IBA
    C-4 methylsterol oxidase activity GO:0000254 TAS
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
Biological Process
    fatty acid metabolic process GO:0006631 TAS
    cholesterol biosynthetic process GO:0006695 IEA
    cholesterol biosynthetic process GO:0006695 TAS
    steroid metabolic process GO:0008202 TAS
    sterol biosynthetic process GO:0016126 IBA
Subcellular Localization
    endoplasmic reticulum GO:0005783 TAS
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
 Experiment description of studies that identified MSMO1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MSMO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Co-fractionation Homo sapiens
2 EIF3A 8661
Co-fractionation Homo sapiens
3 SOX2  
Affinity Capture-MS Homo sapiens
4 ATXN1 6310
Two-hybrid Homo sapiens
5 EIF3F 8665
Co-fractionation Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 VIPR2  
Affinity Capture-MS Homo sapiens
8 FZD7 8324
Affinity Capture-MS Homo sapiens
9 APLNR  
Affinity Capture-MS Homo sapiens
10 CANX 821
Affinity Capture-MS Homo sapiens
11 VIPR1  
Affinity Capture-MS Homo sapiens
12 KNTC1 9735
Cross-Linking-MS (XL-MS) Homo sapiens
13 PDHA2  
Co-fractionation Homo sapiens
14 SYNGR1 9145
Affinity Capture-MS Homo sapiens
15 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SGCA  
Affinity Capture-MS Homo sapiens
18 LRRC25  
Affinity Capture-MS Homo sapiens
19 MICA 100507436
Affinity Capture-MS Homo sapiens
20 ATP5J 522
Affinity Capture-MS Homo sapiens
21 CLEC2D  
Affinity Capture-MS Homo sapiens
22 RTN3 10313
Affinity Capture-MS Homo sapiens
23 BSCL2  
Affinity Capture-MS Homo sapiens
24 TSPAN15 23555
Affinity Capture-MS Homo sapiens
25 FPR1  
Affinity Capture-MS Homo sapiens
26 MGARP  
Affinity Capture-MS Homo sapiens
27 CACNG6  
Affinity Capture-MS Homo sapiens
28 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
29 GAA 2548
Affinity Capture-MS Homo sapiens
30 KLRG2 346689
Affinity Capture-MS Homo sapiens
31 AQP3  
Affinity Capture-MS Homo sapiens
32 MFSD4  
Affinity Capture-MS Homo sapiens
33 P2RY8  
Affinity Capture-MS Homo sapiens
34 FFAR1  
Affinity Capture-MS Homo sapiens
35 TMEM150A  
Affinity Capture-MS Homo sapiens
36 ILVBL 10994
Affinity Capture-MS Homo sapiens
37 GPR35  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 KRTCAP3 200634
Affinity Capture-MS Homo sapiens
39 ARL6IP6  
Affinity Capture-MS Homo sapiens
40 GPR52  
Affinity Capture-MS Homo sapiens
41 GP5 2814
Affinity Capture-MS Homo sapiens
42 FAM134C 162427
Affinity Capture-MS Homo sapiens
43 SARAF  
Affinity Capture-MS Homo sapiens
44 GPR45  
Affinity Capture-MS Homo sapiens
45 P2RY1  
Affinity Capture-MS Homo sapiens
46 PINK1  
Affinity Capture-MS Homo sapiens
47 CHRM5  
Two-hybrid Homo sapiens
48 GPR182  
Affinity Capture-MS Homo sapiens
49 LPAR2 9170
Affinity Capture-MS Homo sapiens
50 ATP5C1 509
Affinity Capture-MS Homo sapiens
51 P2RX5 5026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 LDLR 3949
Negative Genetic Homo sapiens
53 VKORC1 79001
Two-hybrid Homo sapiens
54 DDRGK1 65992
Co-fractionation Homo sapiens
55 TMEM63A 9725
Affinity Capture-MS Homo sapiens
56 HTR1B  
Affinity Capture-MS Homo sapiens
57 FPR2  
Affinity Capture-MS Homo sapiens
58 LPAR1 1902
Affinity Capture-MS Homo sapiens
59 S1PR4  
Affinity Capture-MS Homo sapiens
60 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MSMO1 is involved
PathwayEvidenceSource
Cholesterol biosynthesis TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Metabolism of steroids TAS Reactome





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