Gene description for TMEM63A
Gene name transmembrane protein 63A
Gene symbol TMEM63A
Other names/aliases KIAA0792
Species Homo sapiens
 Database cross references - TMEM63A
ExoCarta ExoCarta_9725
Vesiclepedia VP_9725
Entrez Gene 9725
HGNC 29118
UniProt O94886  
 TMEM63A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for TMEM63A
Molecular Function
    nucleic acid binding GO:0003676 IEA
    calcium-activated cation channel activity GO:0005227 IBA
    protein binding GO:0005515 IPI
    mechanosensitive monoatomic ion channel activity GO:0008381 IDA
    mechanosensitive monoatomic ion channel activity GO:0008381 IMP
    osmolarity-sensing monoatomic cation channel activity GO:1990760 ISS
Biological Process
    monoatomic cation transmembrane transport GO:0098655 IEA
    surfactant secretion GO:0160069 ISS
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    early endosome membrane GO:0031901 IDA
    centriolar satellite GO:0034451 IDA
    specific granule membrane GO:0035579 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified TMEM63A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TMEM63A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 KDELR3 11015
Affinity Capture-MS Homo sapiens
3 SLC29A1 2030
Affinity Capture-MS Homo sapiens
4 NKAIN1  
Affinity Capture-MS Homo sapiens
5 CTAGE7P  
Affinity Capture-MS Homo sapiens
6 NPLOC4 55666
Affinity Capture-MS Homo sapiens
7 TMBIM6 7009
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 RXFP1  
Affinity Capture-MS Homo sapiens
9 OR10H2  
Affinity Capture-MS Homo sapiens
10 SLC45A2 51151
Affinity Capture-MS Homo sapiens
11 KIAA2013 90231
Affinity Capture-MS Homo sapiens
12 RPA2 6118
Proximity Label-MS Homo sapiens
13 SURF4 6836
Affinity Capture-MS Homo sapiens
14 MFSD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PTPN1 5770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TMEM55B 90809
Affinity Capture-MS Homo sapiens
17 KDELR1 10945
Affinity Capture-MS Homo sapiens
18 PTDSS2 81490
Affinity Capture-MS Homo sapiens
19 PIGN 23556
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 AP3S2 10239
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TMEM41B 440026
Affinity Capture-MS Homo sapiens
22 DNAJC11 55735
Affinity Capture-MS Homo sapiens
23 CCR2  
Two-hybrid Homo sapiens
24 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
25 SLC44A5  
Affinity Capture-MS Homo sapiens
26 C3orf52  
Affinity Capture-MS Homo sapiens
27 SLC25A19  
Affinity Capture-MS Homo sapiens
28 LGALS9 3965
Affinity Capture-MS Homo sapiens
29 TMEM63C  
Affinity Capture-MS Homo sapiens
30 MYCN  
Affinity Capture-MS Homo sapiens
31 GPRC5C 55890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DHCR24 1718
Affinity Capture-MS Homo sapiens
33 SLC51A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TMEM87A 25963
Affinity Capture-MS Homo sapiens
35 ZDHHC9  
Affinity Capture-MS Homo sapiens
36 TMEM63B 55362
Affinity Capture-MS Homo sapiens
37 AP3M1 26985
Affinity Capture-MS Homo sapiens
38 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
39 SC5D 6309
Affinity Capture-MS Homo sapiens
40 TMEM126A 84233
Affinity Capture-MS Homo sapiens
41 SFXN3 81855
Affinity Capture-MS Homo sapiens
42 AGTRAP 57085
Affinity Capture-MS Homo sapiens
43 UBAC2 337867
Affinity Capture-MS Homo sapiens
44 TVP23C  
Affinity Capture-MS Homo sapiens
45 CDKAL1  
Affinity Capture-MS Homo sapiens
46 SARAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SLC4A8 9498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RPA4  
Proximity Label-MS Homo sapiens
49 AP3B1 8546
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ATP2C1 27032
Affinity Capture-MS Homo sapiens
51 ARL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 NMUR2  
Affinity Capture-MS Homo sapiens
53 MMGT1 93380
Affinity Capture-MS Homo sapiens
54 SLC4A2 6522
Affinity Capture-MS Homo sapiens
55 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SLC30A2  
Affinity Capture-MS Homo sapiens
58 TTYH1  
Affinity Capture-MS Homo sapiens
59 MSMO1 6307
Affinity Capture-MS Homo sapiens
60 SLC41A3 54946
Affinity Capture-MS Homo sapiens
61 ZDHHC12  
Affinity Capture-MS Homo sapiens
62 GPR89B 51463
Affinity Capture-MS Homo sapiens
63 GHITM 27069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TMEM242  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 OCIAD1 54940
Affinity Capture-MS Homo sapiens
66 TCIRG1 10312
Affinity Capture-MS Homo sapiens
67 MYC  
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 MFSD10 10227
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TMCO1 54499
Affinity Capture-MS Homo sapiens
71 DOLK 22845
Affinity Capture-MS Homo sapiens
72 YIPF4 84272
Affinity Capture-MS Homo sapiens
73 SLC30A7 148867
Affinity Capture-MS Homo sapiens
74 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 GPR37  
Two-hybrid Homo sapiens
76 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 ALG5 29880
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 DRD2  
Two-hybrid Homo sapiens
79 AP3S1 1176
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 MBOAT7 79143
Affinity Capture-MS Homo sapiens
81 SERINC1 57515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SIDT2 51092
Affinity Capture-MS Homo sapiens
83 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 GPR180  
Affinity Capture-MS Homo sapiens
85 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 THEM6  
Affinity Capture-MS Homo sapiens
87 MS4A15  
Affinity Capture-MS Homo sapiens
88 RIOK2 55781
Affinity Capture-MS Homo sapiens
89 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 FAM189A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CISD2 493856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 CCR6  
Affinity Capture-MS Homo sapiens
93 ATP11C 286410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 AP3D1 8943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 CHRNB1  
Affinity Capture-MS Homo sapiens
96 SCAMP3 10067
Affinity Capture-MS Homo sapiens
97 SPTLC2 9517
Affinity Capture-MS Homo sapiens
98 DERL1 79139
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM63A is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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