Gene description for SPTLC2
Gene name serine palmitoyltransferase, long chain base subunit 2
Gene symbol SPTLC2
Other names/aliases HSN1C
LCB2
LCB2A
NSAN1C
SPT2
hLCB2a
Species Homo sapiens
 Database cross references - SPTLC2
ExoCarta ExoCarta_9517
Vesiclepedia VP_9517
Entrez Gene 9517
HGNC 11278
MIM 605713
UniProt O15270  
 SPTLC2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SPTLC2
Molecular Function
    serine C-palmitoyltransferase activity GO:0004758 IDA
    serine C-palmitoyltransferase activity GO:0004758 IDA
    protein binding GO:0005515 IPI
    pyridoxal phosphate binding GO:0030170 IEA
Biological Process
    sphingomyelin biosynthetic process GO:0006686 IEA
    sphingolipid biosynthetic process GO:0030148 IDA
    sphingolipid biosynthetic process GO:0030148 IDA
    sphingolipid biosynthetic process GO:0030148 TAS
    sphinganine biosynthetic process GO:0046511 IEA
    sphingosine biosynthetic process GO:0046512 IBA
    sphingosine biosynthetic process GO:0046512 IDA
    ceramide biosynthetic process GO:0046513 IBA
    ceramide biosynthetic process GO:0046513 IDA
    adipose tissue development GO:0060612 ISS
    positive regulation of lipophagy GO:1904504 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    serine palmitoyltransferase complex GO:0017059 IBA
    serine palmitoyltransferase complex GO:0017059 IDA
    serine palmitoyltransferase complex GO:0017059 IPI
 Experiment description of studies that identified SPTLC2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPTLC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
2 MYCT1 80177
Affinity Capture-MS Homo sapiens
3 P2RX4 5025
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 FASN 2194
Positive Genetic Homo sapiens
6 ALDH3A2 224
Affinity Capture-MS Homo sapiens
7 C4orf32  
Affinity Capture-MS Homo sapiens
8 FAM20C 56975
Affinity Capture-MS Homo sapiens
9 ROM1  
Affinity Capture-MS Homo sapiens
10 RIN3  
Affinity Capture-MS Homo sapiens
11 RIT1 6016
Negative Genetic Homo sapiens
12 ZDHHC12  
Affinity Capture-MS Homo sapiens
13 NELFCD 51497
Affinity Capture-MS Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 BSCL2  
Affinity Capture-MS Homo sapiens
16 SPTLC1 10558
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CUL3 8452
Affinity Capture-MS Homo sapiens
18 C9orf78 51759
Affinity Capture-MS Homo sapiens
19 USP11 8237
Affinity Capture-MS Homo sapiens
20 WBP1  
Affinity Capture-MS Homo sapiens
21 PARK2  
Affinity Capture-MS Homo sapiens
22 FAXC  
Affinity Capture-MS Homo sapiens
23 SCAP  
Affinity Capture-MS Homo sapiens
24 LPAR1 1902
Affinity Capture-MS Homo sapiens
25 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NOTCH3 4854
Reconstituted Complex Homo sapiens
27 PTPRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 RPA3 6119
Proximity Label-MS Homo sapiens
29 PCDHGC4  
Affinity Capture-MS Homo sapiens
30 ATP2A1 487
Proximity Label-MS Homo sapiens
31 NTRK1 4914
Affinity Capture-MS Homo sapiens
32 PCDHB3  
Affinity Capture-MS Homo sapiens
33 LRRTM1 347730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 LPAR2 9170
Affinity Capture-MS Homo sapiens
35 FAM189A2  
Affinity Capture-MS Homo sapiens
36 TGFA  
Affinity Capture-MS Homo sapiens
37 SERBP1 26135
Affinity Capture-MS Homo sapiens
38 EVA1B 55194
Affinity Capture-MS Homo sapiens
39 BCL6  
Affinity Capture-MS Homo sapiens
40 TMEM63A 9725
Affinity Capture-MS Homo sapiens
41 DGCR2 9993
Affinity Capture-MS Homo sapiens
42 PIGH  
Affinity Capture-MS Homo sapiens
43 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TSPAN10 83882
Affinity Capture-MS Homo sapiens
45 C9orf72  
Affinity Capture-MS Homo sapiens
46 C3orf52  
Affinity Capture-MS Homo sapiens
47 DERL1 79139
Proximity Label-MS Homo sapiens
48 PTAR1 375743
Synthetic Lethality Homo sapiens
View the network image/svg+xml
 Pathways in which SPTLC2 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





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