Gene description for SPTLC2
Gene name serine palmitoyltransferase, long chain base subunit 2
Gene symbol SPTLC2
Other names/aliases HSN1C
LCB2
LCB2A
NSAN1C
SPT2
hLCB2a
Species Homo sapiens
 Database cross references - SPTLC2
ExoCarta ExoCarta_9517
Entrez Gene 9517
HGNC 11278
MIM 605713
UniProt O15270  
 SPTLC2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for SPTLC2
Molecular Function
    serine C-palmitoyltransferase activity GO:0004758 IDA
    pyridoxal phosphate binding GO:0030170 IEA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    sphingosine biosynthetic process GO:0046512 IEA
    sphingomyelin biosynthetic process GO:0006686 IEA
    ceramide biosynthetic process GO:0046513 IEA
    sphingolipid metabolic process GO:0006665 TAS
    sphinganine biosynthetic process GO:0046511 IEA
    sphingolipid biosynthetic process GO:0030148 TAS
Subcellular Localization
    serine C-palmitoyltransferase complex GO:0017059 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    mitochondrion GO:0005739 IEA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified SPTLC2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for SPTLC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SPTLC1 10558
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPTLC2 is involved
PathwayEvidenceSource
Sphingolipid de novo biosynthesis TAS Reactome





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