Gene description for P2RX4
Gene name purinergic receptor P2X, ligand gated ion channel, 4
Gene symbol P2RX4
Other names/aliases P2X4
P2X4R
Species Homo sapiens
 Database cross references - P2RX4
ExoCarta ExoCarta_5025
Entrez Gene 5025
HGNC 8535
MIM 600846
UniProt Q99571  
 P2RX4 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for P2RX4
Molecular Function
    copper ion binding GO:0005507 ISS
    extracellular ATP-gated cation channel activity GO:0004931 IDA
    purinergic nucleotide receptor activity GO:0001614 IMP
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 IPI
    zinc ion binding GO:0008270 ISS
    receptor binding GO:0005102 ISS
    protein homodimerization activity GO:0042803 NAS
    drug binding GO:0008144 IEA
    ATP binding GO:0005524 IC
Biological Process
    sensory perception of pain GO:0019233 ISS
    cation transport GO:0006812 IBA
    cellular response to ATP GO:0071318 IDA
    positive regulation of prostaglandin secretion GO:0032308 NAS
    tissue homeostasis GO:0001894 NAS
    ion transmembrane transport GO:0034220 IDA
    positive regulation of nitric oxide biosynthetic process GO:0045429 NAS
    relaxation of cardiac muscle GO:0055119 IMP
    cation transmembrane transport GO:0098655 IEA
    transport GO:0006810 IDA
    endothelial cell activation GO:0042118 TAS
    regulation of blood pressure GO:0008217 IMP
    nitric oxide biosynthetic process GO:0006809 IEA
    regulation of excitatory postsynaptic membrane potential GO:0060079 IEA
    membrane depolarization GO:0051899 IDA
    response to fluid shear stress GO:0034405 IDA
    regulation of cardiac muscle contraction GO:0055117 IMP
    positive regulation of calcium-mediated signaling GO:0050850 IMP
    negative regulation of cardiac muscle hypertrophy GO:0010614 IMP
    positive regulation of calcium ion transport GO:0051928 NAS
    apoptotic signaling pathway GO:0097190 IDA
    calcium ion transport GO:0006816 IEA
    signal transduction GO:0007165 IDA
    purinergic nucleotide receptor signaling pathway GO:0035590 IMP
    vasodilation GO:0042311 IEA
    regulation of sodium ion transport GO:0002028 ISS
    protein homooligomerization GO:0051260 IEA
    positive regulation of calcium ion transport into cytosol GO:0010524 IMP
    neuronal action potential GO:0019228 IEA
    response to ATP GO:0033198 IDA
Subcellular Localization
    membrane GO:0016020 IDA
    integral component of nuclear inner membrane GO:0005639 IBA
    terminal bouton GO:0043195 IEA
    apical part of cell GO:0045177 IEA
    cell junction GO:0030054 IDA
    extracellular exosome GO:0070062 IDA
    postsynaptic density GO:0014069 IEA
    lysosomal membrane GO:0005765 IDA
    neuronal cell body GO:0043025 IEA
    dendritic spine GO:0043197 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    integral component of plasma membrane GO:0005887 IMP
    plasma membrane GO:0005886 ISS
 Experiment description of studies that identified P2RX4 in exosomes
1
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for P2RX4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDH5  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which P2RX4 is involved
No pathways found





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