Gene description for LRRC8E
Gene name leucine rich repeat containing 8 family, member E
Gene symbol LRRC8E
Other names/aliases -
Species Homo sapiens
 Database cross references - LRRC8E
ExoCarta ExoCarta_80131
Vesiclepedia VP_80131
Entrez Gene 80131
HGNC 26272
MIM 612891
UniProt Q6NSJ5  
 LRRC8E identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for LRRC8E
Molecular Function
    volume-sensitive anion channel activity GO:0005225 IDA
    volume-sensitive anion channel activity GO:0005225 IMP
    volume-sensitive anion channel activity GO:0005225 IMP
    protein binding GO:0005515 IPI
Biological Process
    cell volume homeostasis GO:0006884 IDA
    aspartate transmembrane transport GO:0015810 IBA
    aspartate transmembrane transport GO:0015810 IMP
    intracellular signal transduction GO:0035556 IBA
    cellular response to osmotic stress GO:0071470 IMP
    monoatomic anion transmembrane transport GO:0098656 IBA
    monoatomic anion transmembrane transport GO:0098656 IMP
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 IBA
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    monoatomic ion channel complex GO:0034702 IBA
    monoatomic ion channel complex GO:0034702 IMP
 Experiment description of studies that identified LRRC8E in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LRRC8E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPSM2  
Two-hybrid Homo sapiens
2 TUSC2  
Affinity Capture-MS Homo sapiens
3 OR4D6  
Affinity Capture-MS Homo sapiens
4 NPEPPS 9520
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DDHD2 23259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NKAIN1  
Affinity Capture-MS Homo sapiens
7 OR10H1  
Affinity Capture-MS Homo sapiens
8 TRIM3 10612
Two-hybrid Homo sapiens
9 CHRNA4  
Affinity Capture-MS Homo sapiens
10 HSPA6 3310
Affinity Capture-MS Homo sapiens
11 OR10H2  
Affinity Capture-MS Homo sapiens
12 TBC1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HCST  
Affinity Capture-MS Homo sapiens
14 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TRIM2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 TRMT6 51605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ARL6IP6  
Affinity Capture-MS Homo sapiens
18 TM2D3  
Affinity Capture-MS Homo sapiens
19 KCNJ10  
Affinity Capture-MS Homo sapiens
20 HSPA2 3306
Affinity Capture-MS Homo sapiens
21 LYZ 4069
Affinity Capture-MS Homo sapiens
22 CYP19A1  
Affinity Capture-MS Homo sapiens
23 DMD 1756
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PIGH  
Affinity Capture-MS Homo sapiens
25 C3orf52  
Affinity Capture-MS Homo sapiens
26 CCDC47 57003
Affinity Capture-MS Homo sapiens
27 RXFP1  
Affinity Capture-MS Homo sapiens
28 CST1  
Affinity Capture-MS Homo sapiens
29 KCNMB3  
Affinity Capture-MS Homo sapiens
30 TMC6 11322
Affinity Capture-MS Homo sapiens
31 HSPA8 3312
Affinity Capture-MS Homo sapiens
32 IMPDH1 3614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 BAG5 9529
Affinity Capture-MS Homo sapiens
34 TCP1 6950
Affinity Capture-MS Homo sapiens
35 IGHA2 3494
Affinity Capture-MS Homo sapiens
36 OR4N2  
Affinity Capture-MS Homo sapiens
37 BPIFB1 92747
Affinity Capture-MS Homo sapiens
38 PRR4 11272
Affinity Capture-MS Homo sapiens
39 PPME1 51400
Affinity Capture-MS Homo sapiens
40 LCN1 3933
Affinity Capture-MS Homo sapiens
41 SHPK 23729
Affinity Capture-MS Homo sapiens
42 LRRC8B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 NMUR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 P2RX4 5025
Affinity Capture-MS Homo sapiens
46 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TTYH1  
Affinity Capture-MS Homo sapiens
48 CANX 821
Affinity Capture-MS Homo sapiens
49 ICAM2 3384
Affinity Capture-MS Homo sapiens
50 IGHA1 3493
Affinity Capture-MS Homo sapiens
51 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
52 DUOXA2  
Affinity Capture-MS Homo sapiens
53 GP9 2815
Affinity Capture-MS Homo sapiens
54 GPR182  
Affinity Capture-MS Homo sapiens
55 LPAR1 1902
Affinity Capture-MS Homo sapiens
56 TMEM169  
Affinity Capture-MS Homo sapiens
57 NXF1 10482
Affinity Capture-RNA Homo sapiens
58 SSTR1 6751
Affinity Capture-MS Homo sapiens
59 CST4 1472
Affinity Capture-MS Homo sapiens
60 LACRT 90070
Affinity Capture-MS Homo sapiens
61 PSCA 8000
Affinity Capture-MS Homo sapiens
62 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SLC22A4 6583
Affinity Capture-MS Homo sapiens
64 PIGR 5284
Affinity Capture-MS Homo sapiens
65 UPK2 7379
Affinity Capture-MS Homo sapiens
66 SUGT1 10910
Affinity Capture-MS Homo sapiens
67 NUTM1  
Two-hybrid Homo sapiens
68 FCGR3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
70 KCNA2  
Affinity Capture-MS Homo sapiens
71 SAMD4B  
Two-hybrid Homo sapiens
72 LNX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 SIDT2 51092
Affinity Capture-MS Homo sapiens
74 MFSD4  
Affinity Capture-MS Homo sapiens
75 LONP1 9361
Affinity Capture-MS Homo sapiens
76 CLEC2D  
Affinity Capture-MS Homo sapiens
77 TSPAN15 23555
Affinity Capture-MS Homo sapiens
78 FPR2  
Affinity Capture-MS Homo sapiens
79 LRRC55  
Affinity Capture-MS Homo sapiens
80 MOV10 4343
Affinity Capture-RNA Homo sapiens
81 B4GALT4 8702
Affinity Capture-MS Homo sapiens
82 ZG16B 124220
Affinity Capture-MS Homo sapiens
83 PROL1  
Affinity Capture-MS Homo sapiens
84 SGCZ  
Affinity Capture-MS Homo sapiens
85 LTF 4057
Affinity Capture-MS Homo sapiens
86 HSPA1B 3304
Affinity Capture-MS Homo sapiens
87 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LRRC8E is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome
Transport of small molecules TAS Reactome





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