Gene description for DDHD2
Gene name DDHD domain containing 2
Gene symbol DDHD2
Other names/aliases SAMWD1
SPG54
iPLA(1)gamma
Species Homo sapiens
 Database cross references - DDHD2
ExoCarta ExoCarta_23259
Vesiclepedia VP_23259
Entrez Gene 23259
HGNC 29106
MIM 615003
UniProt O94830  
 DDHD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DDHD2
Molecular Function
    phospholipase activity GO:0004620 IBA
    triacylglycerol lipase activity GO:0004806 IBA
    metal ion binding GO:0046872 IEA
    diacylglycerol lipase activity GO:0120516 IEA
Biological Process
    mitochondrial fission GO:0000266 IEA
    locomotory behavior GO:0007626 IEA
    visual learning GO:0008542 IEA
    triglyceride catabolic process GO:0019433 IEA
    lipid droplet organization GO:0034389 IEA
    positive regulation of mitochondrial fission GO:0090141 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IEA
    COPII-coated ER to Golgi transport vesicle GO:0030134 IBA
    centriolar satellite GO:0034451 IDA
 Experiment description of studies that identified DDHD2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DDHD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 REEP5 7905
Proximity Label-MS Homo sapiens
4 HSD17B11 51170
Proximity Label-MS Homo sapiens
5 SEC23IP 11196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 EBAG9 9166
Proximity Label-MS Homo sapiens
7 C2orf68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 METTL7A 25840
Proximity Label-MS Homo sapiens
10 RAB5A 5868
Proximity Label-MS Homo sapiens
11 ALB 213
Affinity Capture-MS Homo sapiens
12 MTCH2 23788
Proximity Label-MS Homo sapiens
13 CP 1356
Affinity Capture-MS Homo sapiens
14 MTNR1A  
Two-hybrid Homo sapiens
15 LAMP2 3920
Proximity Label-MS Homo sapiens
16 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 EEF1A2 1917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TF 7018
Affinity Capture-MS Homo sapiens
20 HP 3240
Affinity Capture-MS Homo sapiens
21 RCCD1  
Affinity Capture-MS Homo sapiens
22 TGM4 7047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DYRK1A 1859
Affinity Capture-MS Homo sapiens
24 RAPGEF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 BLVRA 644
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RAB11A 8766
Proximity Label-MS Homo sapiens
27 LRRC8E 80131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 EMILIN1 11117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 IGHG1 3500
Affinity Capture-MS Homo sapiens
30 PCDH10 57575
Affinity Capture-MS Homo sapiens
31 SIDT2 51092
Affinity Capture-MS Homo sapiens
32 ALOX5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ELOVL5 60481
Proximity Label-MS Homo sapiens
34 CHI3L1 1116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 DERL1 79139
Proximity Label-MS Homo sapiens
36 ATP2A1 487
Proximity Label-MS Homo sapiens
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