Experiment description of studies that identified IGHG1 in sEVs

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

6 Experiment ID 51 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 14975938     Organism Homo sapiens Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions. Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN" Journal name AJRCMB Publication year 2004 Sample Malignant pleural effusions Sample name Pleural Fluid - Breast cancer Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Western blotting

7 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

8 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

9 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

11 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

12 Experiment ID 902 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ AMY|ALB|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33432243     Organism Homo sapiens Experiment description Exosome detection via the ultrafast-isolation system: EXODUS Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F." Journal name Nat Methods Publication year 2021 Sample Saliva Sample name Saliva Isolation/purification methods Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

13 Experiment ID 903 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ AMY|ALB|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33432243     Organism Homo sapiens Experiment description Exosome detection via the ultrafast-isolation system: EXODUS Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F." Journal name Nat Methods Publication year 2021 Sample Saliva Sample name Saliva Isolation/purification methods Filtration Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

Protein-protein interactions for IGHG1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 DDHD2 23259 Affinity Capture-MS Homo sapiens 3 SPRTN   Affinity Capture-MS Homo sapiens 4 SHC1 6464 Affinity Capture-MS Homo sapiens 5 NRSN1   Affinity Capture-MS Homo sapiens 6 YWHAE 7531 Affinity Capture-MS Homo sapiens 7 ALB 213 Affinity Capture-MS Homo sapiens 8 ALDH2 217 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 AGA 175 Affinity Capture-MS Homo sapiens 10 ZNF354C   Affinity Capture-MS Homo sapiens 11 EBF2   Affinity Capture-MS Homo sapiens 12 RNF208   Affinity Capture-MS Homo sapiens 13 FNDC5   Affinity Capture-MS Homo sapiens 14 APOD 347 Affinity Capture-MS Homo sapiens 15 RHBDD1 84236 Affinity Capture-MS Homo sapiens 16 POTEF 728378 Affinity Capture-MS Homo sapiens 17 KIAA1429 25962 Affinity Capture-MS Homo sapiens 18 WTAP 9589 Affinity Capture-MS Homo sapiens 19 CCNA2 890 Affinity Capture-MS Homo sapiens 20 E2F6 1876 Affinity Capture-MS Homo sapiens 21 MCM2 4171 Affinity Capture-MS Homo sapiens 22 CA8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 EGFL8   Affinity Capture-MS Homo sapiens 24 SNRPC 6631 Affinity Capture-MS Homo sapiens 25 YWHAG 7532 Affinity Capture-MS Homo sapiens 26 UGT1A10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 SFXN4   Affinity Capture-MS Homo sapiens 28 CYP3A5   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 EED   Affinity Capture-MS Homo sapiens 30 UCHL5 51377 Reconstituted Complex Homo sapiens 31 PHF11 51131 Affinity Capture-MS Homo sapiens 32 VCAM1 7412 Affinity Capture-MS Homo sapiens 33 FNDC1 84624 Affinity Capture-MS Homo sapiens 34 ZSCAN20   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 SNX27 81609 Affinity Capture-MS Homo sapiens 36 MYCN   Affinity Capture-MS Homo sapiens 37 ICE2   Affinity Capture-MS Homo sapiens 38 C18orf21   Affinity Capture-MS Homo sapiens 39 OBSL1 23363 Affinity Capture-MS Homo sapiens 40 DDX19B 11269 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 DDX31   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 GNG8   Affinity Capture-MS Homo sapiens 43 ARL2BP 23568 Affinity Capture-MS Homo sapiens 44 USP11 8237 Affinity Capture-MS Homo sapiens 45 ST6GALNAC6 30815 Affinity Capture-MS Homo sapiens 46 PARK2   Affinity Capture-MS Homo sapiens 47 LRRC10   Affinity Capture-MS Homo sapiens 48 FUNDC2 65991 Affinity Capture-MS Homo sapiens 49 CERS3   Affinity Capture-MS Homo sapiens 50 RNF115   Affinity Capture-MS Homo sapiens 51 SRRT 51593 Affinity Capture-MS Homo sapiens 52 CCDC51 79714 Affinity Capture-MS Homo sapiens 53 MATN2 4147 Affinity Capture-MS Homo sapiens 54 TRIM21 6737 Reconstituted Complex Homo sapiens Co-crystal Structure Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 55 SARAF   Affinity Capture-MS Homo sapiens 56 PIGT 51604 Affinity Capture-MS Homo sapiens 57 SLX4   Affinity Capture-MS Homo sapiens 58 FRAS1 80144 Affinity Capture-MS Homo sapiens 59 SSUH2   Affinity Capture-MS Homo sapiens 60 GDPD1 284161 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 61 YWHAQ 10971 Affinity Capture-MS Homo sapiens 62 ALDH3A1 218 Affinity Capture-MS Homo sapiens 63 HCN1   Affinity Capture-MS Homo sapiens 64 UPK1A 11045 Affinity Capture-MS Homo sapiens 65 DDX39B 7919 Affinity Capture-MS Homo sapiens 66 ENC1   Affinity Capture-MS Homo sapiens 67 CYLD   Affinity Capture-MS Homo sapiens 68 UPP1 7378 Affinity Capture-MS Homo sapiens 69 TMPO 7112 Affinity Capture-MS Homo sapiens 70 BRF1   Affinity Capture-MS Homo sapiens 71 GPR141   Affinity Capture-MS Homo sapiens 72 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 73 FANCD2   Affinity Capture-MS Homo sapiens 74 METTL14   Affinity Capture-MS Homo sapiens 75 IGHG4 3503 Affinity Capture-MS Homo sapiens 76 AGPAT1 10554 Affinity Capture-MS Homo sapiens 77 ZFPL1 7542 Affinity Capture-MS Homo sapiens 78 ASCC1 51008 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 79 IKZF3   Affinity Capture-MS Homo sapiens 80 TBC1D22B   Affinity Capture-MS Homo sapiens 81 ITLN2   Affinity Capture-MS Homo sapiens 82 IQCB1   Affinity Capture-MS Homo sapiens 83 CIR1   Affinity Capture-MS Homo sapiens 84 BTF3 689 Affinity Capture-MS Homo sapiens 85 EGFR 1956 Affinity Capture-MS Homo sapiens 86 ZC3HC1   Affinity Capture-MS Homo sapiens 87 HSD17B10 3028 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 88 MORF4L1   Affinity Capture-MS Homo sapiens 89 IFNA4   Affinity Capture-MS Homo sapiens 90 TFG 10342 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 91 TADA2A   Affinity Capture-MS Homo sapiens 92 NEFM 4741 Affinity Capture-MS Homo sapiens 93 SPOP   Affinity Capture-MS Homo sapiens 94 CSNK2A2 1459 Affinity Capture-MS Homo sapiens 95 EEF1A2 1917 Affinity Capture-MS Homo sapiens 96 IKZF1   Affinity Capture-MS Homo sapiens 97 AZGP1 563 Affinity Capture-MS Homo sapiens 98 STX17 55014 Affinity Capture-MS Homo sapiens 99 RIPPLY3   Affinity Capture-MS Homo sapiens 100 UBE2U   Affinity Capture-MS Homo sapiens 101 CCBL1 883 Affinity Capture-MS Homo sapiens 102 SUSD3 203328 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 103 FCGR3B   Co-crystal Structure Homo sapiens 104 FAM175B 23172 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 105 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 106 SLC25A6 293 Affinity Capture-MS Homo sapiens 107 SCGB1D1   Affinity Capture-MS Homo sapiens 108 RBM24 221662 Affinity Capture-MS Homo sapiens 109 MICU3   Affinity Capture-MS Homo sapiens 110 EMC4 51234 Affinity Capture-MS Homo sapiens 111 NHLRC2 374354 Affinity Capture-MS Homo sapiens 112 SSBP2   Affinity Capture-MS Homo sapiens 113 IGHG2 3501 Affinity Capture-MS Homo sapiens 114 PTDSS1 9791 Affinity Capture-MS Homo sapiens 115 EDC3 80153 Affinity Capture-MS Homo sapiens 116 MKI67   Affinity Capture-MS Homo sapiens 117 PDE6D 5147 Affinity Capture-MS Homo sapiens 118 MBNL1 4154 Affinity Capture-MS Homo sapiens 119 KLK10 5655 Affinity Capture-MS Homo sapiens 120 PINK1   Affinity Capture-MS Homo sapiens 121 GOT1 2805 Affinity Capture-MS Homo sapiens 122 METTL3   Affinity Capture-MS Homo sapiens 123 IGHG3 3502 Affinity Capture-MS Homo sapiens 124 PDE4DIP 9659 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 125 CUL7 9820 Affinity Capture-MS Homo sapiens 126 SMIM8   Affinity Capture-MS Homo sapiens 127 OR2A4 79541 Affinity Capture-MS Homo sapiens 128 GABPA 2551 Affinity Capture-MS Homo sapiens 129 CCR1   Affinity Capture-MS Homo sapiens 130 P2RX6   Affinity Capture-MS Homo sapiens 131 EP300 2033 Affinity Capture-MS Homo sapiens View the network

Pathways in which IGHG1 is involved

No pathways found