Gene description for AZGP1
Gene name alpha-2-glycoprotein 1, zinc-binding
Gene symbol AZGP1
Other names/aliases ZA2G
ZAG
Species Homo sapiens
 Database cross references - AZGP1
ExoCarta ExoCarta_563
Vesiclepedia VP_563
Entrez Gene 563
HGNC 910
MIM 194460
UniProt P25311  
 AZGP1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 19199708    
Serum 25265333    
Serum 25265333    
Urine 21557262    
 Gene ontology annotations for AZGP1
Molecular Function
    RNA nuclease activity GO:0004540 NAS
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 NAS
Biological Process
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IDA
    positive regulation of T cell mediated cytotoxicity GO:0001916 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class Ib GO:0002476 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent GO:0002486 IBA
    immune response GO:0006955 IBA
    cell adhesion GO:0007155 IDA
    negative regulation of cell population proliferation GO:0008285 NAS
    protein transmembrane transport GO:0071806 IEA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 HDA
    external side of plasma membrane GO:0009897 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified AZGP1 in exosomes
1
Experiment ID 494
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 266
MISEV standards
Biophysical techniques
TSG101|Alix
Enriched markers
CANX|HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 267
MISEV standards
Biophysical techniques
TSG101|Alix
Enriched markers
CANX|HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 268
MISEV standards
Biophysical techniques
TSG101|Alix
Enriched markers
CANX|HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
10
Experiment ID 269
MISEV standards
Biophysical techniques
Alix
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Serum
Sample name Serum - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 270
MISEV standards
Biophysical techniques
Alix
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Serum
Sample name Serum - HBV infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 114
MISEV standards
IEM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AZGP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUF2  
Two-hybrid Homo sapiens
2 GK3P  
Affinity Capture-MS Homo sapiens
3 STATH  
Affinity Capture-MS Homo sapiens
4 ATG9A 79065
Proximity Label-MS Homo sapiens
5 RPS19BP1  
Affinity Capture-MS Homo sapiens
6 MBD4  
Affinity Capture-MS Homo sapiens
7 EBF2  
Affinity Capture-MS Homo sapiens
8 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ST7  
Affinity Capture-MS Homo sapiens
10 SOX2  
Affinity Capture-MS Homo sapiens
11 FNDC5  
Affinity Capture-MS Homo sapiens
12 HPX 3263
Affinity Capture-MS Homo sapiens
13 MCM2 4171
Affinity Capture-MS Homo sapiens
14 ACPP 55
Affinity Capture-MS Homo sapiens
15 ORM1 5004
Affinity Capture-MS Homo sapiens
16 NUDT3 11165
Affinity Capture-MS Homo sapiens
17 EFEMP1 2202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KPRP 448834
Affinity Capture-MS Homo sapiens
19 Ikbip  
Affinity Capture-MS Mus musculus
20 SERPINA1 5265
Affinity Capture-MS Homo sapiens
21 IFNA4  
Affinity Capture-MS Homo sapiens
22 USP53 54532
Affinity Capture-MS Homo sapiens
23 TDRKH  
Affinity Capture-MS Homo sapiens
24 HAT1 8520
Affinity Capture-MS Homo sapiens
25 ZYX 7791
Affinity Capture-MS Homo sapiens
26 ZNF550  
Affinity Capture-MS Homo sapiens
27 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 GNG8  
Affinity Capture-MS Homo sapiens
29 WHSC1 7468
Affinity Capture-MS Homo sapiens
30 MYCBPAP 84073
Affinity Capture-MS Homo sapiens
31 STX17 55014
Affinity Capture-MS Homo sapiens
32 ARG1 383
Affinity Capture-MS Homo sapiens
33 MSMB  
Affinity Capture-MS Homo sapiens
34 RASSF9  
Affinity Capture-MS Homo sapiens
35 FANCG 2189
Affinity Capture-MS Homo sapiens
36 ITLN2  
Affinity Capture-MS Homo sapiens
37 IGHG1 3500
Affinity Capture-MS Homo sapiens
38 SLX4  
Affinity Capture-MS Homo sapiens
39 S100A8 6279
Affinity Capture-MS Homo sapiens
40 SPRR2E  
Affinity Capture-MS Homo sapiens
41 GK  
Affinity Capture-MS Homo sapiens
42 PRR4 11272
Affinity Capture-MS Homo sapiens
43 ALDH3A1 218
Affinity Capture-MS Homo sapiens
44 LCN1 3933
Affinity Capture-MS Homo sapiens
45 SREBF2 6721
Negative Genetic Homo sapiens
46 DDX39B 7919
Affinity Capture-MS Homo sapiens
47 GJB5  
Affinity Capture-MS Homo sapiens
48 GTPBP4 23560
Affinity Capture-MS Homo sapiens
49 CYLD  
Affinity Capture-MS Homo sapiens
50 C1orf68 100129271
Affinity Capture-MS Homo sapiens
51 SERPINB12 89777
Affinity Capture-MS Homo sapiens
52 CASP14 23581
Affinity Capture-MS Homo sapiens
53 SARAF  
Affinity Capture-MS Homo sapiens
54 SERPINB3 6317
Affinity Capture-MS Homo sapiens
55 FANCD2  
Affinity Capture-MS Homo sapiens
56 VWA3B 200403
Affinity Capture-MS Homo sapiens
57 CCNC  
Affinity Capture-MS Homo sapiens
58 C16orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 ASCC1 51008
Affinity Capture-MS Homo sapiens
60 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 TMEM176B 28959
Affinity Capture-MS Homo sapiens
62 UBQLN2 29978
Two-hybrid Homo sapiens
63 CD274 29126
Affinity Capture-MS Homo sapiens
64 CDK19 23097
Affinity Capture-MS Homo sapiens
65 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
66 SEMG2 6407
Affinity Capture-MS Homo sapiens
67 FTH1 2495
Affinity Capture-MS Homo sapiens
68 LOR  
Affinity Capture-MS Homo sapiens
69 SYDE1 85360
Affinity Capture-MS Homo sapiens
70 SEMG1 6406
Affinity Capture-MS Homo sapiens
71 CDK2 1017
Affinity Capture-MS Homo sapiens
72 PI4KAP1  
Affinity Capture-MS Homo sapiens
73 KLK3  
Affinity Capture-MS Homo sapiens
74 RASSF10  
Affinity Capture-MS Homo sapiens
75 DNAJC9 23234
Affinity Capture-MS Homo sapiens
76 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
77 ABCC1 4363
Two-hybrid Homo sapiens
78 CUL7 9820
Affinity Capture-MS Homo sapiens
79 SEH1L 81929
Affinity Capture-MS Homo sapiens
80 DET1  
Affinity Capture-MS Homo sapiens
81 PHF8  
Affinity Capture-MS Homo sapiens
82 MMRN1 22915
Affinity Capture-MS Homo sapiens
83 ARHGEF40 55701
Affinity Capture-MS Homo sapiens
84 RIPPLY3  
Affinity Capture-MS Homo sapiens
85 PRSS16 10279
Affinity Capture-MS Homo sapiens
86 PSMB10 5699
Affinity Capture-MS Homo sapiens
87 ANXA2 302
Affinity Capture-MS Homo sapiens
88 KIF14 9928
Affinity Capture-MS Homo sapiens
89 SHBG 6462
Two-hybrid Homo sapiens
90 AMTN  
Affinity Capture-MS Homo sapiens
91 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 SCGB1D1  
Affinity Capture-MS Homo sapiens
93 SH2D3A  
Affinity Capture-MS Homo sapiens
94 MCM5 4174
Affinity Capture-MS Homo sapiens
95 ZNF154  
Affinity Capture-MS Homo sapiens
96 TIMM10  
Affinity Capture-MS Homo sapiens
97 DSC1 1823
Affinity Capture-MS Homo sapiens
98 FKBP14  
Affinity Capture-MS Homo sapiens
99 ERCC3  
Affinity Capture-MS Homo sapiens
100 TEFM  
Affinity Capture-MS Homo sapiens
101 TSPAN6 7105
Affinity Capture-MS Homo sapiens
102 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 PRKX 5613
Affinity Capture-MS Homo sapiens
104 ARHGAP23 57636
Affinity Capture-MS Homo sapiens
105 ATP11C 286410
Affinity Capture-MS Homo sapiens
106 NUCKS1 64710
Affinity Capture-MS Homo sapiens
107 GABPA 2551
Affinity Capture-MS Homo sapiens
108 CCR1  
Affinity Capture-MS Homo sapiens
109 GALNT9  
Affinity Capture-MS Homo sapiens
110 ECSIT 51295
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AZGP1 is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome
Transport of small molecules TAS Reactome





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