Experiment description of studies that identified DSC1 in exosomes

1 Experiment ID 494 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ EV Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 266 MISEV standards ✘ EV Biophysical techniques ✔ TSG101|Alix EV Enriched markers ✔ CANX|HSP90B1 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25265333     Organism Homo sapiens Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J Journal name J Proteome Res Publication year 2014 Sample Liver cancer cells Sample name Huh-7 - Wild type Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 268 MISEV standards ✘ EV Biophysical techniques ✔ TSG101|Alix EV Enriched markers ✔ CANX|HSP90B1 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25265333     Organism Homo sapiens Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J Journal name J Proteome Res Publication year 2014 Sample Liver cancer cells Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 224 MISEV standards ✔ EM|AFM EV Biophysical techniques ✔ Alix|TSG101|CD63|CD81 EV Enriched markers ✔ GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

6 Experiment ID 231 MISEV standards ✘ EV Biophysical techniques ✔ Alix|CD63|CD9 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 232 MISEV standards ✘ EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 233 MISEV standards ✘ EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for DSC1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PKP2 5318 Two-hybrid Homo sapiens 2 DSG2 1829 Invivo Homo sapiens Invitro Homo sapiens 3 PKP3 11187 Two-hybrid Homo sapiens 4 JUP 3728 Invitro Homo sapiens 5 DSP 1832 Invitro Homo sapiens View the network

Pathways in which DSC1 is involved

No pathways found