Gene description for PKP1
Gene name plakophilin 1
Gene symbol PKP1
Other names/aliases B6P
Species Homo sapiens
 Database cross references - PKP1
ExoCarta ExoCarta_5317
Vesiclepedia VP_5317
Entrez Gene 5317
HGNC 9023
MIM 601975
UniProt Q13835  
 PKP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for PKP1
Molecular Function
    DNA binding GO:0003677 IDA
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 IDA
    intermediate filament binding GO:0019215 NAS
    structural constituent of skin epidermis GO:0030280 NAS
    cadherin binding GO:0045296 IBA
Biological Process
    desmosome assembly GO:0002159 ISS
    desmosome maintenance GO:0002160 IEA
    cell adhesion GO:0007155 NAS
    signal transduction GO:0007165 NAS
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of cell-cell adhesion GO:0022409 IMP
    positive regulation of cell-cell adhesion GO:0022409 ISS
    transepithelial water transport GO:0035377 IEA
    ameloblast differentiation GO:0036305 ISS
    intermediate filament bundle assembly GO:0045110 IDA
    positive regulation of keratinocyte differentiation GO:0045618 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    cell-cell adhesion GO:0098609 IBA
    negative regulation of mRNA catabolic process GO:1902373 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 ISS
    positive regulation of cap-dependent translational initiation GO:1903676 IMP
    positive regulation of protein localization to membrane GO:1905477 IMP
Subcellular Localization
    cornified envelope GO:0001533 TAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    intermediate filament GO:0005882 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 IBA
    cytoplasmic stress granule GO:0010494 IEA
    desmosome GO:0030057 IDA
    desmosome GO:0030057 NAS
    perinuclear region of cytoplasm GO:0048471 IDA
    nuclear stress granule GO:0097165 IDA
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified PKP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PKP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 METTL7A 25840
Affinity Capture-MS Homo sapiens
3 NRSN1  
Affinity Capture-MS Homo sapiens
4 TARDBP 23435
Affinity Capture-MS Homo sapiens
5 ALDH2 217
Affinity Capture-MS Homo sapiens
6 FNDC5  
Affinity Capture-MS Homo sapiens
7 CTSD 1509
Affinity Capture-MS Homo sapiens
8 GPATCH2L  
Affinity Capture-MS Homo sapiens
9 MCM2 4171
Affinity Capture-MS Homo sapiens
10 CA8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 FBXW7  
Affinity Capture-MS Homo sapiens
12 CD109 135228
Affinity Capture-MS Homo sapiens
13 CPLX3  
Affinity Capture-MS Homo sapiens
14 CIDEA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FLG 2312
Affinity Capture-MS Homo sapiens
16 PHF8  
Affinity Capture-MS Homo sapiens
17 PHF11 51131
Affinity Capture-MS Homo sapiens
18 RSG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 OAZ3  
Affinity Capture-MS Homo sapiens
20 SERPINB5 5268
Affinity Capture-MS Homo sapiens
21 KIF3A 11127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SGOL2 151246
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NDUFS5 4725
Affinity Capture-MS Homo sapiens
24 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 KRT8 3856
Reconstituted Complex Homo sapiens
26 POF1B 79983
Affinity Capture-MS Homo sapiens
27 COASY 80347
Affinity Capture-MS Homo sapiens
28 DSG1 1828
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
29 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
30 FOXI2  
Affinity Capture-MS Homo sapiens
31 ZDHHC19  
Affinity Capture-MS Homo sapiens
32 ARMC8 25852
Two-hybrid Homo sapiens
33 SRRT 51593
Affinity Capture-MS Homo sapiens
34 PEMT  
Affinity Capture-MS Homo sapiens
35 CCDC51 79714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 WIPI1 55062
Affinity Capture-MS Homo sapiens
37 KRT14 3861
Reconstituted Complex Homo sapiens
38 ANXA9  
Affinity Capture-MS Homo sapiens
39 HCN1  
Affinity Capture-MS Homo sapiens
40 DDX39B 7919
Affinity Capture-MS Homo sapiens
41 UCHL5 51377
Reconstituted Complex Homo sapiens
42 CYLD  
Affinity Capture-MS Homo sapiens
43 FSTL1 11167
Affinity Capture-MS Homo sapiens
44 FBXL4 26235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CASP14 23581
Affinity Capture-MS Homo sapiens
46 SERPINB3 6317
Affinity Capture-MS Homo sapiens
47 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
48 IVL 3713
Affinity Capture-MS Homo sapiens
49 FANCD2  
Affinity Capture-MS Homo sapiens
50 CCDC82  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 JADE1  
Affinity Capture-MS Homo sapiens
52 ZC3HC1  
Affinity Capture-MS Homo sapiens
53 RBFOX1 54715
Affinity Capture-MS Homo sapiens
54 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
55 PLD5  
Affinity Capture-MS Homo sapiens
56 SUZ12  
Affinity Capture-MS Homo sapiens
57 TFG 10342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SSUH2  
Affinity Capture-MS Homo sapiens
59 PI4KAP1  
Affinity Capture-MS Homo sapiens
60 FOS 2353
Affinity Capture-MS Homo sapiens
61 MYC  
Affinity Capture-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 BLMH 642
Affinity Capture-MS Homo sapiens
64 TNS2 23371
Two-hybrid Homo sapiens
65 BMI1  
Affinity Capture-MS Homo sapiens
66 SLC37A4 2542
Affinity Capture-MS Homo sapiens
67 C18orf21  
Affinity Capture-MS Homo sapiens
68 STX17 55014
Affinity Capture-MS Homo sapiens
69 CCDC8  
Affinity Capture-MS Homo sapiens
70 DSP 1832
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
71 SPICE1  
Affinity Capture-MS Homo sapiens
72 RBM24 221662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 TUBG1 7283
Affinity Capture-MS Homo sapiens
74 GOSR1 9527
Affinity Capture-MS Homo sapiens
75 POLR2L 5441
Affinity Capture-MS Homo sapiens
76 DDX3X 1654
Affinity Capture-MS Homo sapiens
77 MICU3  
Affinity Capture-MS Homo sapiens
78 USP1 7398
Affinity Capture-MS Homo sapiens
79 CCR1  
Affinity Capture-MS Homo sapiens
80 CAPZA2 830
Affinity Capture-MS Homo sapiens
81 DSC1 1823
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
82 PTDSS1 9791
Affinity Capture-MS Homo sapiens
83 MBNL1 4154
Affinity Capture-MS Homo sapiens
84 OPN5  
Affinity Capture-MS Homo sapiens
85 TGM3 7053
Affinity Capture-MS Homo sapiens
86 KDELR2 11014
Affinity Capture-MS Homo sapiens
87 PMVK 10654
Affinity Capture-MS Homo sapiens
88 SERPINB12 89777
Affinity Capture-MS Homo sapiens
89 PINK1  
Affinity Capture-MS Homo sapiens
90 GOT1 2805
Affinity Capture-MS Homo sapiens
91 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
92 OR2A4 79541
Affinity Capture-MS Homo sapiens
93 ANXA2 302
Affinity Capture-MS Homo sapiens
94 VIM 7431
Reconstituted Complex Homo sapiens
95 GABPA 2551
Affinity Capture-MS Homo sapiens
96 TEX10 54881
Affinity Capture-MS Homo sapiens
97 PRMT1 3276
Affinity Capture-MS Homo sapiens
98 P2RX6  
Affinity Capture-MS Homo sapiens
99 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
100 FLG2 388698
Affinity Capture-MS Homo sapiens
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