Gene description for SERPINB12
Gene name serpin peptidase inhibitor, clade B (ovalbumin), member 12
Gene symbol SERPINB12
Other names/aliases YUKOPIN
Species Homo sapiens
 Database cross references - SERPINB12
ExoCarta ExoCarta_89777
Vesiclepedia VP_89777
Entrez Gene 89777
HGNC 14220
MIM 615662
UniProt Q96P63  
 SERPINB12 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SERPINB12
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IMP
    enzyme binding GO:0019899 NAS
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    negative regulation of endopeptidase activity GO:0010951 IBA
    negative regulation of protein catabolic process GO:0042177 NAS
Subcellular Localization
    cornified envelope GO:0001533 IEA
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 NAS
    plasma membrane GO:0005886 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified SERPINB12 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SERPINB12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1orf68 100129271
Affinity Capture-MS Homo sapiens
2 JUP 3728
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 CASP14 23581
Affinity Capture-MS Homo sapiens
5 MYCN  
Affinity Capture-MS Homo sapiens
6 UTY  
Affinity Capture-MS Homo sapiens
7 TP53 7157
Affinity Capture-MS Homo sapiens
8 BAX 581
Affinity Capture-MS Homo sapiens
9 FANCD2  
Affinity Capture-MS Homo sapiens
10 Cdc23  
Affinity Capture-MS Mus musculus
11 METTL14  
Affinity Capture-MS Homo sapiens
12 RBM8A 9939
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 S100A14 57402
Affinity Capture-MS Homo sapiens
15 Pds5a  
Affinity Capture-MS Mus musculus
16 ANXA2 302
Affinity Capture-MS Homo sapiens
17 LLGL1 3996
Affinity Capture-MS Homo sapiens
18 CALML5 51806
Affinity Capture-MS Homo sapiens
19 POLR2L 5441
Affinity Capture-MS Homo sapiens
20 DDX3X 1654
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Affinity Capture-MS Homo sapiens
22 KPNB1 3837
Affinity Capture-MS Homo sapiens
23 RPA1 6117
Affinity Capture-MS Homo sapiens
24 Nrip3  
Affinity Capture-MS Mus musculus
25 CD274 29126
Affinity Capture-MS Homo sapiens
26 SRP19 6728
Affinity Capture-MS Homo sapiens
27 DSG1 1828
Affinity Capture-MS Homo sapiens
28 DSC1 1823
Affinity Capture-MS Homo sapiens
29 EGFR 1956
Affinity Capture-MS Homo sapiens
30 CSTA 1475
Affinity Capture-MS Homo sapiens
31 THOC5 8563
Affinity Capture-MS Homo sapiens
32 PRSS3 5646
Affinity Capture-MS Homo sapiens
33 DRG1 4733
Affinity Capture-MS Homo sapiens
34 ARG1 383
Affinity Capture-MS Homo sapiens
35 PKP1 5317
Affinity Capture-MS Homo sapiens
36 SOX2  
Affinity Capture-MS Homo sapiens
37 FNDC5  
Affinity Capture-MS Homo sapiens
38 APTX  
Affinity Capture-MS Homo sapiens
39 AP2M1 1173
Affinity Capture-MS Homo sapiens
40 MOGAT3  
Affinity Capture-MS Homo sapiens
41 COQ2  
Affinity Capture-MS Homo sapiens
42 RNF38  
Affinity Capture-MS Homo sapiens
43 PRMT1 3276
Affinity Capture-MS Homo sapiens
44 EZH2  
Affinity Capture-MS Homo sapiens
45 FABP5 2171
Affinity Capture-MS Homo sapiens
46 RPA3 6119
Affinity Capture-MS Homo sapiens
47 SLX4  
Affinity Capture-MS Homo sapiens
48 MCM2 4171
Affinity Capture-MS Homo sapiens
49 DCD 117159
Affinity Capture-MS Homo sapiens
50 BLMH 642
Affinity Capture-MS Homo sapiens
51 SOSTDC1  
Affinity Capture-MS Homo sapiens
52 FBXW7  
Affinity Capture-MS Homo sapiens
53 B3GNT2 10678
Affinity Capture-MS Homo sapiens
54 BMI1  
Affinity Capture-MS Homo sapiens
55 HRNR 388697
Affinity Capture-MS Homo sapiens
56 TGM3 7053
Affinity Capture-MS Homo sapiens
57 CUL2 8453
Affinity Capture-MS Homo sapiens
58 AZGP1 563
Affinity Capture-MS Homo sapiens
59 DDX39B 7919
Affinity Capture-MS Homo sapiens
60 HECW2  
Affinity Capture-MS Homo sapiens
61 DSP 1832
Affinity Capture-MS Homo sapiens
62 RAD51  
Affinity Capture-MS Homo sapiens
63 CYLD  
Affinity Capture-MS Homo sapiens
64 CUL4A 8451
Affinity Capture-MS Homo sapiens
65 CCDC8  
Affinity Capture-MS Homo sapiens
66 KPRP 448834
Affinity Capture-MS Homo sapiens
67 UCHL5 51377
Reconstituted Complex Homo sapiens
68 FLG2 388698
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SERPINB12 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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