Experiment description of studies that identified C1orf68 in sEVs

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for C1orf68

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 Fam134a   Affinity Capture-MS Mus musculus 3 CAT 847 Affinity Capture-MS Homo sapiens 4 SERPINB12 89777 Affinity Capture-MS Homo sapiens 5 CASP14 23581 Affinity Capture-MS Homo sapiens 6 MYCN   Affinity Capture-MS Homo sapiens 7 METTL14   Affinity Capture-MS Homo sapiens 8 ESR1   Affinity Capture-MS Homo sapiens 9 TGM3 7053 Affinity Capture-MS Homo sapiens 10 CHMP4C 92421 Affinity Capture-MS Homo sapiens 11 RLIM 51132 Affinity Capture-MS Homo sapiens 12 PRSS3 5646 Affinity Capture-MS Homo sapiens 13 CD274 29126 Affinity Capture-MS Homo sapiens 14 DSG1 1828 Affinity Capture-MS Homo sapiens 15 DSC1 1823 Affinity Capture-MS Homo sapiens 16 HNRNPA1 3178 Affinity Capture-MS Homo sapiens 17 AZGP1 563 Affinity Capture-MS Homo sapiens 18 S100A14 57402 Affinity Capture-MS Homo sapiens 19 JUP 3728 Affinity Capture-MS Homo sapiens 20 WTAP 9589 Affinity Capture-MS Homo sapiens 21 ARG1 383 Affinity Capture-MS Homo sapiens 22 B3GNT2 10678 Affinity Capture-MS Homo sapiens 23 MYC   Affinity Capture-MS Homo sapiens 24 TRIM21 6737 Affinity Capture-MS Homo sapiens 25 BLMH 642 Affinity Capture-MS Homo sapiens 26 METTL3   Affinity Capture-MS Homo sapiens 27 ANXA2 302 Affinity Capture-MS Homo sapiens 28 DDX39B 7919 Affinity Capture-MS Homo sapiens 29 NELFE 7936 Affinity Capture-MS Homo sapiens 30 CYLD   Affinity Capture-MS Homo sapiens 31 KPRP 448834 Affinity Capture-MS Homo sapiens 32 DSP 1832 Affinity Capture-MS Homo sapiens 33 FLG2 388698 Affinity Capture-MS Homo sapiens View the network

Pathways in which C1orf68 is involved

No pathways found