Gene description for NELFE
Gene name negative elongation factor complex member E
Gene symbol NELFE
Other names/aliases D6S45
NELF-E
RD
RDBP
RDP
Species Homo sapiens
 Database cross references - NELFE
ExoCarta ExoCarta_7936
Vesiclepedia VP_7936
Entrez Gene 7936
HGNC 13974
MIM 154040
UniProt P18615  
 NELFE identified in exosomes derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NELFE
Molecular Function
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 NAS
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    positive regulation of protein modification process GO:0031401 IEA
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 IBA
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    plasma membrane GO:0005886 IDA
    nuclear body GO:0016604 IDA
    NELF complex GO:0032021 IBA
    NELF complex GO:0032021 IDA
    NELF complex GO:0032021 IPI
 Experiment description of studies that identified NELFE in exosomes
1
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NELFE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ENO3 2027
Affinity Capture-MS Homo sapiens
2 PSMA6 5687
Co-fractionation Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 C5AR1  
Affinity Capture-MS Homo sapiens
5 AP2B1 163
Affinity Capture-MS Homo sapiens
6 NCBP1 4686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 LYZ 4069
Affinity Capture-MS Homo sapiens
8 ESD 2098
Affinity Capture-MS Homo sapiens
9 BRDT  
Affinity Capture-MS Homo sapiens
10 PTMA 5757
Affinity Capture-MS Homo sapiens
11 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
12 SRPK2 6733
Biochemical Activity Homo sapiens
13 CTBP2 1488
Affinity Capture-MS Homo sapiens
14 PSMD11 5717
Co-fractionation Homo sapiens
15 S100A6 6277
Affinity Capture-MS Homo sapiens
16 YWHAE 7531
Affinity Capture-MS Homo sapiens
17 PRSS3 5646
Affinity Capture-MS Homo sapiens
18 ACTC1 70
Proximity Label-MS Homo sapiens
19 C19orf43  
Affinity Capture-MS Homo sapiens
20 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
21 PPIA 5478
Affinity Capture-MS Homo sapiens
22 KPNA2 3838
Affinity Capture-MS Homo sapiens
23 IGLV2-23  
Affinity Capture-MS Homo sapiens
24 CFL1 1072
Affinity Capture-MS Homo sapiens
25 HIST2H2BF 440689
Affinity Capture-MS Homo sapiens
26 SOX9  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 SRSF8 10929
Affinity Capture-MS Homo sapiens
28 DECR1 1666
Affinity Capture-MS Homo sapiens
29 RNH1 6050
Affinity Capture-MS Homo sapiens
30 RUVBL1 8607
Affinity Capture-MS Homo sapiens
31 WDR48 57599
Affinity Capture-MS Homo sapiens
32 PRDX3 10935
Affinity Capture-MS Homo sapiens
33 IGHMBP2 3508
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TPM3 7170
Affinity Capture-MS Homo sapiens
35 SF3B1 23451
Affinity Capture-MS Homo sapiens
36 Nelfa  
Affinity Capture-MS Mus musculus
37 H2AFV 94239
Affinity Capture-MS Homo sapiens
38 HIST2H3A 333932
Affinity Capture-MS Homo sapiens
39 FBXW7  
Synthetic Lethality Homo sapiens
40 NPM1 4869
Affinity Capture-MS Homo sapiens
41 PSMB6 5694
Co-fractionation Homo sapiens
42 YWHAG 7532
Affinity Capture-MS Homo sapiens
43 MTUS2 23281
Two-hybrid Homo sapiens
44 RSRC1  
Affinity Capture-MS Homo sapiens
45 NCOR1  
Two-hybrid Homo sapiens
46 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
47 SNRPD3 6634
Affinity Capture-MS Homo sapiens
48 ATG16L1 55054
Affinity Capture-MS Homo sapiens
49 POLR2C 5432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CLTC 1213
Affinity Capture-MS Homo sapiens
51 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
52 H2AFY2 55506
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
53 NBN 4683
Affinity Capture-MS Homo sapiens
54 ACIN1 22985
Affinity Capture-MS Homo sapiens
55 FEN1 2237
Affinity Capture-MS Homo sapiens
56 YWHAB 7529
Affinity Capture-MS Homo sapiens
57 POLR2K  
Affinity Capture-MS Homo sapiens
58 PFN1 5216
Affinity Capture-MS Homo sapiens
59 XYLT1 64131
Affinity Capture-MS Homo sapiens
60 RBBP7 5931
Affinity Capture-MS Homo sapiens
61 FOXC1  
Affinity Capture-MS Homo sapiens
62 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
63 PSMB4 5692
Co-fractionation Homo sapiens
64 DDX21 9188
Affinity Capture-MS Homo sapiens
65 ACOT9 23597
Affinity Capture-MS Homo sapiens
66 RPL4 6124
Affinity Capture-MS Homo sapiens
67 SUPT5H 6829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SNAI2  
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
69 TNKS 8658
Reconstituted Complex Homo sapiens
70 CBX3 11335
Affinity Capture-MS Homo sapiens
71 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
72 CNTNAP4 85445
Affinity Capture-MS Homo sapiens
73 SNRPB 6628
Affinity Capture-MS Homo sapiens
74 SNRPD1 6632
Affinity Capture-MS Homo sapiens
75 NELFE 7936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 RAN 5901
Affinity Capture-MS Homo sapiens
77 NTRK1 4914
Affinity Capture-MS Homo sapiens
78 DNAJA1 3301
Affinity Capture-MS Homo sapiens
79 LUC7L2 51631
Affinity Capture-MS Homo sapiens
80 YWHAQ 10971
Affinity Capture-MS Homo sapiens
81 SRSF1 6426
Affinity Capture-MS Homo sapiens
82 ENO1 2023
Affinity Capture-MS Homo sapiens
83 SUB1 10923
Affinity Capture-MS Homo sapiens
84 PDIA6 10130
Affinity Capture-MS Homo sapiens
85 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
86 CEBPA  
Protein-peptide Homo sapiens
87 SRPK1 6732
Biochemical Activity Homo sapiens
88 Ncbp2  
Affinity Capture-MS Mus musculus
89 C1orf68 100129271
Affinity Capture-MS Homo sapiens
90 POLR2B 5431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 BCLAF1 9774
Affinity Capture-MS Homo sapiens
92 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
93 HNRNPD 3184
Affinity Capture-MS Homo sapiens
94 HSPA5 3309
Affinity Capture-MS Homo sapiens
95 PSMA3 5684
Co-fractionation Homo sapiens
96 OAT 4942
Affinity Capture-MS Homo sapiens
97 TAF15 8148
Affinity Capture-MS Homo sapiens
98 KHSRP 8570
Affinity Capture-MS Homo sapiens
99 BAG1 573
Affinity Capture-MS Homo sapiens
100 PCIF1 63935
Affinity Capture-MS Homo sapiens
101 NCBP2 22916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 SMARCA5 8467
Affinity Capture-MS Homo sapiens
103 DHX15 1665
Affinity Capture-MS Homo sapiens
104 PSMD13 5719
Co-fractionation Homo sapiens
105 NELFCD 51497
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
106 CCDC57 284001
Two-hybrid Homo sapiens
107 POLR2E 5434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 VAMP3 9341
Two-hybrid Homo sapiens
109 PHF2  
Affinity Capture-MS Homo sapiens
110 YWHAZ 7534
Affinity Capture-MS Homo sapiens
111 SPECC1 92521
Affinity Capture-MS Homo sapiens
112 POLR2D  
Affinity Capture-MS Homo sapiens
113 LOR  
Affinity Capture-MS Homo sapiens
114 WIBG 84305
Co-fractionation Homo sapiens
115 IBTK 25998
Affinity Capture-MS Homo sapiens
116 ACSS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
117 FABP5 2171
Affinity Capture-MS Homo sapiens
118 RPA3 6119
Proximity Label-MS Homo sapiens
119 COX6C 1345
Affinity Capture-MS Homo sapiens
120 CYP2J2  
Affinity Capture-MS Homo sapiens
121 SET 6418
Affinity Capture-MS Homo sapiens
122 DIS3 22894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 TRIM27  
Two-hybrid Homo sapiens
124 PPA2 27068
Affinity Capture-MS Homo sapiens
125 ENO2 2026
Affinity Capture-MS Homo sapiens
126 SF3B3 23450
Affinity Capture-MS Homo sapiens
127 CACTIN  
Two-hybrid Homo sapiens
128 NELFB 25920
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
129 INTS5 80789
Affinity Capture-MS Homo sapiens
130 POLR2A 5430
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
131 SUPT16H 11198
Affinity Capture-MS Homo sapiens
132 ASUN  
Affinity Capture-MS Homo sapiens
133 PPIF 10105
Affinity Capture-MS Homo sapiens
134 HSPE1 3336
Affinity Capture-MS Homo sapiens
135 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
136 FASN 2194
Positive Genetic Homo sapiens
137 SPTAN1 6709
Affinity Capture-MS Homo sapiens
138 POLR2F  
Affinity Capture-MS Homo sapiens
139 TMEM141  
Affinity Capture-MS Homo sapiens
140 C11orf58  
Affinity Capture-MS Homo sapiens
141 HMGB1 3146
Affinity Capture-MS Homo sapiens
142 NUCKS1 64710
Affinity Capture-MS Homo sapiens
143 CRABP2 1382
Affinity Capture-MS Homo sapiens
144 MEN1 4221
Affinity Capture-MS Homo sapiens
145 LCK 3932
Affinity Capture-MS Homo sapiens
146 RPAP2  
Affinity Capture-MS Homo sapiens
147 THRAP3 9967
Affinity Capture-MS Homo sapiens
148 CMTR1 23070
Affinity Capture-MS Homo sapiens
149 NELFA  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
150 CHD4 1108
Affinity Capture-MS Homo sapiens
151 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
152 PUF60 22827
Affinity Capture-MS Homo sapiens
153 PTBP2  
Affinity Capture-MS Homo sapiens
154 PSMA1 5682
Co-fractionation Homo sapiens
155 PCNA 5111
Affinity Capture-MS Homo sapiens
156 NCL 4691
Affinity Capture-MS Homo sapiens
157 PSMA5 5686
Co-fractionation Homo sapiens
158 WDR76  
Affinity Capture-MS Homo sapiens
159 PRDX6 9588
Affinity Capture-MS Homo sapiens
160 TRAP1 10131
Affinity Capture-MS Homo sapiens
161 SRSF7 6432
Affinity Capture-MS Homo sapiens
162 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 TTC29  
Affinity Capture-MS Homo sapiens
164 ACO2 50
Affinity Capture-MS Homo sapiens
165 HNRNPF 3185
Affinity Capture-MS Homo sapiens
166 STARD10 10809
Affinity Capture-MS Homo sapiens
167 AR 367
Proximity Label-MS Homo sapiens
168 ANXA2 302
Affinity Capture-MS Homo sapiens
169 SSRP1 6749
Affinity Capture-MS Homo sapiens
170 POLR2G 5436
Affinity Capture-MS Homo sapiens
171 PRMT1 3276
Affinity Capture-MS Homo sapiens
172 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NELFE is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat IEA Reactome
Disease IEA Reactome
Disease TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat TAS Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle IEA Reactome
HIV Life Cycle TAS Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Elongation TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Late Phase of HIV Life Cycle IEA Reactome
Late Phase of HIV Life Cycle TAS Reactome
Pausing and recovery of HIV elongation IEA Reactome
Pausing and recovery of Tat-mediated HIV elongation IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription of the HIV genome TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome





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