Gene description for NELFE
Gene name negative elongation factor complex member E
Gene symbol NELFE
Other names/aliases D6S45
NELF-E
RD
RDBP
RDP
Species Homo sapiens
 Database cross references - NELFE
ExoCarta ExoCarta_7936
Entrez Gene 7936
HGNC 13974
MIM 154040
UniProt P18615  
 NELFE identified in exosomes derived from the following tissue/cell type
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for NELFE
Molecular Function
    protein binding GO:0005515 IPI
    RNA binding GO:0003723 NAS
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
Biological Process
    negative regulation of mRNA polyadenylation GO:1900364 IMP
    gene expression GO:0010467 TAS
    negative regulation of transcription elongation from RNA polymerase II promoter GO:0034244 IEA
    biological_process GO:0008150 ND
    transcription from RNA polymerase II promoter GO:0006366 TAS
    positive regulation of viral transcription GO:0050434 TAS
    viral process GO:0016032 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
Subcellular Localization
    NELF complex GO:0032021 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified NELFE in exosomes
1
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NELFE
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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