Gene description for FECH
Gene name ferrochelatase
Gene symbol FECH
Other names/aliases EPP
FCE
Species Homo sapiens
 Database cross references - FECH
ExoCarta ExoCarta_2235
Vesiclepedia VP_2235
Entrez Gene 2235
HGNC 3647
MIM 612386
UniProt P22830  
 FECH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for FECH
Molecular Function
    ferrochelatase activity GO:0004325 IBA
    ferrochelatase activity GO:0004325 IDA
    protein binding GO:0005515 IPI
    ferrous iron binding GO:0008198 TAS
    heme binding GO:0020037 IEA
    iron-responsive element binding GO:0030350 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    2 iron, 2 sulfur cluster binding GO:0051537 IEA
Biological Process
    generation of precursor metabolites and energy GO:0006091 TAS
    heme biosynthetic process GO:0006783 IBA
    heme biosynthetic process GO:0006783 IDA
    heme A biosynthetic process GO:0006784 IEA
    heme B biosynthetic process GO:0006785 IEA
    cholesterol metabolic process GO:0008203 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to light stimulus GO:0009416 TAS
    detection of UV GO:0009589 IEA
    response to lead ion GO:0010288 IEA
    response to insecticide GO:0017085 IEA
    erythrocyte differentiation GO:0030218 IEA
    very-low-density lipoprotein particle assembly GO:0034379 IEA
    response to ethanol GO:0045471 IEA
    protoporphyrinogen IX metabolic process GO:0046501 IDA
    response to arsenic-containing substance GO:0046685 IEA
    regulation of hemoglobin biosynthetic process GO:0046984 IEA
    heme O biosynthetic process GO:0048034 IEA
    response to methylmercury GO:0051597 IEA
    multicellular organismal-level iron ion homeostasis GO:0060586 IEA
    response to platinum ion GO:0070541 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified FECH in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FECH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
3 CYP1A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MME 4311
Affinity Capture-MS Homo sapiens
5 TRUB2  
Proximity Label-MS Homo sapiens
6 GAPDHS 26330
Affinity Capture-MS Homo sapiens
7 C9orf78 51759
Affinity Capture-MS Homo sapiens
8 MCCC1 56922
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CLUH 23277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CCDC90B  
Proximity Label-MS Homo sapiens
12 DNAJB6 10049
Affinity Capture-MS Homo sapiens
13 C7orf55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MCU 90550
Proximity Label-MS Homo sapiens
15 PMPCA 23203
Proximity Label-MS Homo sapiens
16 COQ9  
Affinity Capture-MS Homo sapiens
17 COPS6 10980
Affinity Capture-MS Homo sapiens
18 MRPL23 6150
Affinity Capture-MS Homo sapiens
19 COPS5 10987
Affinity Capture-MS Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 YARS2  
Affinity Capture-MS Homo sapiens
22 HINT2 84681
Proximity Label-MS Homo sapiens
23 MRPS24 64951
Affinity Capture-MS Homo sapiens
24 CALM3 808
Affinity Capture-MS Homo sapiens
25 POU5F1  
Affinity Capture-MS Homo sapiens
26 FTSJ2 29960
Affinity Capture-MS Homo sapiens
27 MALSU1  
Affinity Capture-MS Homo sapiens
28 PDK2 5164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 GNA15 2769
Affinity Capture-MS Homo sapiens
30 AMD1  
Affinity Capture-MS Homo sapiens
31 RCBTB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 C8orf76  
Affinity Capture-MS Homo sapiens
33 FAHD2B  
Affinity Capture-MS Homo sapiens
34 CALM2 805
Affinity Capture-MS Homo sapiens
35 C6orf203  
Proximity Label-MS Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 NR1H3  
Affinity Capture-MS Homo sapiens
38 AMACR 23600
Affinity Capture-MS Homo sapiens
39 TUFM 7284
Proximity Label-MS Homo sapiens
40 NIT1 4817
Affinity Capture-MS Homo sapiens
41 PPP2CB 5516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NELFE 7936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RPA2 6118
Proximity Label-MS Homo sapiens
44 CCDC109B 55013
Proximity Label-MS Homo sapiens
45 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
46 CBX6  
Affinity Capture-MS Homo sapiens
47 MRPL11 65003
Proximity Label-MS Homo sapiens
48 MTRF1L  
Proximity Label-MS Homo sapiens
49 AQP12B  
Affinity Capture-MS Homo sapiens
50 MMP26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TMEM70  
Proximity Label-MS Homo sapiens
52 ABCB7 22
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
53 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
54 MINK1 50488
Affinity Capture-MS Homo sapiens
55 C15orf48  
Affinity Capture-MS Homo sapiens
56 PHIP 55023
Affinity Capture-MS Homo sapiens
57 USP42  
Affinity Capture-MS Homo sapiens
58 AK4 205
Affinity Capture-MS Homo sapiens
59 MINOS1  
Affinity Capture-MS Homo sapiens
60 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 TMOD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 NELFCD 51497
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 B3GNT3 10331
Affinity Capture-MS Homo sapiens
64 PRSS50  
Affinity Capture-MS Homo sapiens
65 ERCC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
66 NR3C1 2908
Proximity Label-MS Homo sapiens
67 CTTN 2017
Affinity Capture-MS Homo sapiens
68 EGFR 1956
Negative Genetic Homo sapiens
69 FAHD1 81889
Affinity Capture-MS Homo sapiens
70 FBXO6 26270
Affinity Capture-MS Homo sapiens
71 RIPK4  
Affinity Capture-MS Homo sapiens
72 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 IL12RB1  
Affinity Capture-MS Homo sapiens
75 TMOD3 29766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ATPAF2 91647
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 NELFB 25920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 SIRT3  
Affinity Capture-MS Homo sapiens
80 MCUR1 63933
Proximity Label-MS Homo sapiens
81 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
82 CLPP 8192
Proximity Label-MS Homo sapiens
83 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
84 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
85 FASN 2194
Negative Genetic Homo sapiens
86 AUH 549
Proximity Label-MS Homo sapiens
87 ROCK1 6093
Affinity Capture-MS Homo sapiens
88 PGRMC1 10857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 UQCC1 55245
Affinity Capture-MS Homo sapiens
90 ICT1 3396
Proximity Label-MS Homo sapiens
91 UBL4B  
Affinity Capture-MS Homo sapiens
92 PDHA1 5160
Proximity Label-MS Homo sapiens
93 NDUFS7 374291
Affinity Capture-MS Homo sapiens
94 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
95 COX8A  
Proximity Label-MS Homo sapiens
96 C21orf33  
Proximity Label-MS Homo sapiens
97 SSBP1 6742
Proximity Label-MS Homo sapiens
98 ERCC6  
Affinity Capture-Western Homo sapiens
99 GFM1 85476
Proximity Label-MS Homo sapiens
100 FECH 2235
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
101 TRIM74  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 C1QBP 708
Proximity Label-MS Homo sapiens
103 SERBP1 26135
Affinity Capture-MS Homo sapiens
104 C9orf72  
Affinity Capture-MS Homo sapiens
105 TIMP2 7077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 USP20  
Affinity Capture-MS Homo sapiens
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