Gene description for PDK2
Gene name pyruvate dehydrogenase kinase, isozyme 2
Gene symbol PDK2
Other names/aliases PDHK2
PDKII
Species Homo sapiens
 Database cross references - PDK2
ExoCarta ExoCarta_5164
Vesiclepedia VP_5164
Entrez Gene 5164
HGNC 8810
MIM 602525
UniProt Q15119  
 PDK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDK2
Molecular Function
    protein kinase activity GO:0004672 TAS
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IBA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein homodimerization activity GO:0042803 IPI
Biological Process
    glucose metabolic process GO:0006006 TAS
    regulation of gluconeogenesis GO:0006111 ISS
    regulation of pH GO:0006885 ISS
    insulin receptor signaling pathway GO:0008286 ISS
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 IBA
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 ISS
    regulation of ketone metabolic process GO:0010565 ISS
    regulation of glucose metabolic process GO:0010906 IBA
    regulation of glucose metabolic process GO:0010906 ISS
    cellular response to nutrient GO:0031670 ISS
    cellular response to reactive oxygen species GO:0034614 IMP
    glucose homeostasis GO:0042593 ISS
    regulation of calcium-mediated signaling GO:0050848 IEA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
    pyruvate dehydrogenase complex GO:0045254 ISS
 Experiment description of studies that identified PDK2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACAD9 28976
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PDK1 5163
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 VSIG4  
Two-hybrid Homo sapiens
4 FOXD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SYT12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 STAT5A 6776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
8 PMPCA 23203
Proximity Label-MS Homo sapiens
9 PDHA1 5160
Proximity Label-MS Homo sapiens
10 ADCK2  
Affinity Capture-MS Homo sapiens
11 DLAT 1737
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 AARS2  
Proximity Label-MS Homo sapiens
13 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PDK3 5165
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PYCRL 65263
Affinity Capture-MS Homo sapiens
16 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PDHX 8050
Reconstituted Complex Homo sapiens
18 PDK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 Hdac6  
Affinity Capture-MS Mus musculus
20 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
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