Gene description for PDK2
Gene name pyruvate dehydrogenase kinase, isozyme 2
Gene symbol PDK2
Other names/aliases PDHK2
PDKII
Species Homo sapiens
 Database cross references - PDK2
ExoCarta ExoCarta_5164
Vesiclepedia VP_5164
Entrez Gene 5164
HGNC 8810
MIM 602525
UniProt Q15119  
 PDK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDK2
Molecular Function
    ATP binding GO:0005524 IDA
    protein kinase activity GO:0004672 TAS
    protein homodimerization activity GO:0042803 IPI
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IDA
Biological Process
    insulin receptor signaling pathway GO:0008286 ISS
    cellular response to nutrient GO:0031670 ISS
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
    small molecule metabolic process GO:0044281 TAS
    cellular response to reactive oxygen species GO:0034614 IMP
    protein phosphorylation GO:0006468 TAS
    pyruvate metabolic process GO:0006090 TAS
    regulation of pH GO:0006885 ISS
    regulation of cellular ketone metabolic process GO:0010565 ISS
    glucose metabolic process GO:0006006 IEA
    regulation of glucose metabolic process GO:0010906 ISS
    glucose homeostasis GO:0042593 ISS
    cellular metabolic process GO:0044237 TAS
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 TAS
    regulation of gluconeogenesis GO:0006111 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    nucleoplasm GO:0005654 IDA
    mitochondrial pyruvate dehydrogenase complex GO:0005967 ISS
 Experiment description of studies that identified PDK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DLAT 1737
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 PDK1 5163
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 PDHX 8050
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PDK2 is involved
PathwayEvidenceSource
Regulation of pyruvate dehydrogenase (PDH) complex TAS Reactome
Signaling by Retinoic Acid TAS Reactome





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