Gene description for DLAT
Gene name dihydrolipoamide S-acetyltransferase
Gene symbol DLAT
Other names/aliases DLTA
PDC-E2
PDCE2
Species Homo sapiens
 Database cross references - DLAT
ExoCarta ExoCarta_1737
Entrez Gene 1737
HGNC 2896
MIM 608770
UniProt P10515  
 DLAT identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Neuroblastoma cells 25944692    
 Gene ontology annotations for DLAT
Molecular Function
    dihydrolipoyllysine-residue acetyltransferase activity GO:0004742 NAS
    protein binding GO:0005515 IPI
Biological Process
    small molecule metabolic process GO:0044281 TAS
    pyruvate metabolic process GO:0006090 TAS
    cellular metabolic process GO:0044237 TAS
    glucose metabolic process GO:0006006 IEA
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 TAS
    tricarboxylic acid cycle GO:0006099 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    mitochondrial pyruvate dehydrogenase complex GO:0005967 NAS
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified DLAT in exosomes
1
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DLAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHX  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 PDK2  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
3 PDK1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 PDHA1 5160
Affinity Capture-MS Homo sapiens
5 PDK3  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 PDHB 5162
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
7 PDK4  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here