Gene description for HSCB
Gene name HscB mitochondrial iron-sulfur cluster co-chaperone
Gene symbol HSCB
Other names/aliases DNAJC20
HSC20
JAC1
dJ366L4.2
Species Homo sapiens
 Database cross references - HSCB
ExoCarta ExoCarta_150274
Vesiclepedia VP_150274
Entrez Gene 150274
HGNC 28913
MIM 608142
UniProt Q8IWL3  
 HSCB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HSCB
Molecular Function
    ATPase activator activity GO:0001671 IEA
    molecular_function GO:0003674 ND
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    protein-folding chaperone binding GO:0051087 IEA
Biological Process
    iron-sulfur cluster assembly GO:0016226 IMP
    [2Fe-2S] cluster assembly GO:0044571 IBA
    protein complex oligomerization GO:0051259 IEA
    primitive hemopoiesis GO:0060215 ISS
    primitive erythrocyte differentiation GO:0060319 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified HSCB in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HSCB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Proximity Label-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 AFG3L2 10939
Proximity Label-MS Homo sapiens
4 SLIRP 81892
Proximity Label-MS Homo sapiens
5 MRPL48  
Proximity Label-MS Homo sapiens
6 NDUFS4 4724
Proximity Label-MS Homo sapiens
7 SARS2 54938
Proximity Label-MS Homo sapiens
8 SNAP23 8773
Proximity Label-MS Homo sapiens
9 NDUFS1 4719
Proximity Label-MS Homo sapiens
10 MRPS27 23107
Proximity Label-MS Homo sapiens
11 ALDH2 217
Proximity Label-MS Homo sapiens
12 MRPL45 84311
Proximity Label-MS Homo sapiens
13 MRPL24  
Proximity Label-MS Homo sapiens
14 MRPL10 124995
Proximity Label-MS Homo sapiens
15 ETFB 2109
Proximity Label-MS Homo sapiens
16 NDUFS7 374291
Proximity Label-MS Homo sapiens
17 MRPL50 54534
Proximity Label-MS Homo sapiens
18 GATB  
Proximity Label-MS Homo sapiens
19 GRPEL1 80273
Affinity Capture-MS Homo sapiens
20 MRPL49 740
Proximity Label-MS Homo sapiens
21 MRPS14  
Proximity Label-MS Homo sapiens
22 NDUFS2 4720
Proximity Label-MS Homo sapiens
23 CARS2 79587
Proximity Label-MS Homo sapiens
24 LRPPRC 10128
Proximity Label-MS Homo sapiens
25 MRPL20 55052
Proximity Label-MS Homo sapiens
26 NDUFS3 4722
Proximity Label-MS Homo sapiens
27 OGDH 4967
Proximity Label-MS Homo sapiens
28 TFAM 7019
Proximity Label-MS Homo sapiens
29 PDK1 5163
Proximity Label-MS Homo sapiens
30 IARS2 55699
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
31 UBE2O 63893
Affinity Capture-MS Homo sapiens
32 NDUFA7 4701
Proximity Label-MS Homo sapiens
33 MRPL23 6150
Proximity Label-MS Homo sapiens
34 MRPS5 64969
Proximity Label-MS Homo sapiens
35 HINT2 84681
Proximity Label-MS Homo sapiens
36 MRPS23 51649
Proximity Label-MS Homo sapiens
37 NDUFA5 4698
Proximity Label-MS Homo sapiens
38 MRPL12 6182
Proximity Label-MS Homo sapiens
39 ETFA 2108
Proximity Label-MS Homo sapiens
40 MRPS26 64949
Proximity Label-MS Homo sapiens
41 POLRMT 5442
Proximity Label-MS Homo sapiens
42 PTCD1 26024
Proximity Label-MS Homo sapiens
43 ERAL1  
Proximity Label-MS Homo sapiens
44 MRPS25 64432
Proximity Label-MS Homo sapiens
45 MRPL39 54148
Proximity Label-MS Homo sapiens
46 NDUFV1 4723
Proximity Label-MS Homo sapiens
47 NDUFV3 4731
Proximity Label-MS Homo sapiens
48 Gspt1 14852
Affinity Capture-MS Mus musculus
49 ATP5H 10476
Proximity Label-MS Homo sapiens
50 SLC30A9 10463
Proximity Label-MS Homo sapiens
51 BCS1L 617
Proximity Label-MS Homo sapiens
52 MRPL44  
Proximity Label-MS Homo sapiens
53 MRPL4 51073
Proximity Label-MS Homo sapiens
54 FASTKD2  
Proximity Label-MS Homo sapiens
55 PREPL 9581
Proximity Label-MS Homo sapiens
56 NDUFA6  
Proximity Label-MS Homo sapiens
57 VWA8 23078
Proximity Label-MS Homo sapiens
58 MRPL47 57129
Proximity Label-MS Homo sapiens
59 MRPS17 51373
Proximity Label-MS Homo sapiens
60 MTERF3  
Proximity Label-MS Homo sapiens
61 CDK5RAP1  
Proximity Label-MS Homo sapiens
62 MDH2 4191
Proximity Label-MS Homo sapiens
63 STOML2 30968
Proximity Label-MS Homo sapiens
64 ALAS1  
Proximity Label-MS Homo sapiens
65 ATP5J 522
Proximity Label-MS Homo sapiens
66 NDUFV2 4729
Proximity Label-MS Homo sapiens
67 SDHA 6389
Proximity Label-MS Homo sapiens
68 THEM4  
Proximity Label-MS Homo sapiens
69 FASTKD5  
Proximity Label-MS Homo sapiens
70 ISCU 23479
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
71 MRPS34 65993
Proximity Label-MS Homo sapiens
72 NT5DC2 64943
Proximity Label-MS Homo sapiens
73 ATPAF1  
Proximity Label-MS Homo sapiens
74 AGTRAP 57085
Two-hybrid Homo sapiens
75 PTCD3 55037
Proximity Label-MS Homo sapiens
76 MMAB 326625
Proximity Label-MS Homo sapiens
77 MRPS6  
Proximity Label-MS Homo sapiens
78 PDHX 8050
Proximity Label-MS Homo sapiens
79 POLDIP2 26073
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
80 MRPL3  
Proximity Label-MS Homo sapiens
81 LYRM4 57128
Proximity Label-MS Homo sapiens
82 MRPL22  
Proximity Label-MS Homo sapiens
83 NDUFAF3 25915
Proximity Label-MS Homo sapiens
84 ATP5B 506
Proximity Label-MS Homo sapiens
85 MRPL2 51069
Proximity Label-MS Homo sapiens
86 COX4I1 1327
Proximity Label-MS Homo sapiens
87 MRPL41 64975
Proximity Label-MS Homo sapiens
88 NDUFAF1  
Proximity Label-MS Homo sapiens
89 OXA1L  
Proximity Label-MS Homo sapiens
90 ECH1 1891
Proximity Label-MS Homo sapiens
91 PDE12 201626
Proximity Label-MS Homo sapiens
92 ACOT1 641371
Proximity Label-MS Homo sapiens
93 MRPL40 64976
Proximity Label-MS Homo sapiens
94 EARS2  
Proximity Label-MS Homo sapiens
95 MRPL21 219927
Proximity Label-MS Homo sapiens
96 HADHA 3030
Proximity Label-MS Homo sapiens
97 MTIF2 4528
Proximity Label-MS Homo sapiens
98 TRMT10C 54931
Proximity Label-MS Homo sapiens
99 PNPT1 87178
Proximity Label-MS Homo sapiens
100 MRPS35 60488
Proximity Label-MS Homo sapiens
101 NDUFA9 4704
Proximity Label-MS Homo sapiens
102 MRPS11  
Proximity Label-MS Homo sapiens
103 LETM1 3954
Proximity Label-MS Homo sapiens
104 MRPS7 51081
Proximity Label-MS Homo sapiens
105 MRPL27 51264
Proximity Label-MS Homo sapiens
106 MRPL9 65005
Proximity Label-MS Homo sapiens
107 RSU1 6251
Affinity Capture-MS Homo sapiens
108 NFS1 9054
Proximity Label-MS Homo sapiens
109 PYCR1 5831
Proximity Label-MS Homo sapiens
110 HSD17B10 3028
Proximity Label-MS Homo sapiens
111 ELAC2 60528
Proximity Label-MS Homo sapiens
112 DHX30 22907
Proximity Label-MS Homo sapiens
113 VARS2  
Proximity Label-MS Homo sapiens
114 PYCR2 29920
Proximity Label-MS Homo sapiens
115 ECHS1 1892
Proximity Label-MS Homo sapiens
116 C17orf80 55028
Proximity Label-MS Homo sapiens
117 MRPL15 29088
Proximity Label-MS Homo sapiens
118 GATC  
Proximity Label-MS Homo sapiens
119 MRPL1  
Proximity Label-MS Homo sapiens
120 TACO1  
Proximity Label-MS Homo sapiens
121 MRPL37 51253
Proximity Label-MS Homo sapiens
122 DLST 1743
Proximity Label-MS Homo sapiens
123 HSDL2 84263
Proximity Label-MS Homo sapiens
124 RPL35A 6165
Proximity Label-MS Homo sapiens
125 DAP3 7818
Proximity Label-MS Homo sapiens
126 ACAD9 28976
Proximity Label-MS Homo sapiens
127 MRPS36 92259
Proximity Label-MS Homo sapiens
128 PDPR 55066
Proximity Label-MS Homo sapiens
129 QRSL1  
Proximity Label-MS Homo sapiens
130 NDUFA12 55967
Proximity Label-MS Homo sapiens
131 HSPD1 3329
Proximity Label-MS Homo sapiens
132 MRPL38  
Proximity Label-MS Homo sapiens
133 DLAT 1737
Proximity Label-MS Homo sapiens
134 C10orf2 56652
Proximity Label-MS Homo sapiens
135 MTHFD1L 25902
Proximity Label-MS Homo sapiens
136 AK4 205
Proximity Label-MS Homo sapiens
137 CLPP 8192
Proximity Label-MS Homo sapiens
138 GLS 2744
Proximity Label-MS Homo sapiens
139 ARL2 402
Proximity Label-MS Homo sapiens
140 GLUD1 2746
Proximity Label-MS Homo sapiens
141 CLPX 10845
Proximity Label-MS Homo sapiens
142 NDUFAF2  
Proximity Label-MS Homo sapiens
143 PDK3 5165
Proximity Label-MS Homo sapiens
144 MRPS2 51116
Proximity Label-MS Homo sapiens
145 COQ5  
Proximity Label-MS Homo sapiens
146 ICT1 3396
Proximity Label-MS Homo sapiens
147 MRPS28  
Proximity Label-MS Homo sapiens
148 PDHB 5162
Proximity Label-MS Homo sapiens
149 NME4 4833
Proximity Label-MS Homo sapiens
150 MRPS31  
Proximity Label-MS Homo sapiens
151 MRPL43 84545
Proximity Label-MS Homo sapiens
152 ACADSB 36
Proximity Label-MS Homo sapiens
153 DCTN2 10540
Affinity Capture-MS Homo sapiens
154 CLUH 23277
Proximity Label-MS Homo sapiens
155 MRPS10 55173
Proximity Label-MS Homo sapiens
156 SUCLA2 8803
Proximity Label-MS Homo sapiens
157 SHMT2 6472
Proximity Label-MS Homo sapiens
158 MRPS21  
Proximity Label-MS Homo sapiens
159 SSBP1 6742
Proximity Label-MS Homo sapiens
160 OAT 4942
Proximity Label-MS Homo sapiens
161 ATAD3A 55210
Proximity Label-MS Homo sapiens
162 ME2 4200
Proximity Label-MS Homo sapiens
163 NIPSNAP1 8508
Proximity Label-MS Homo sapiens
164 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
165 NDUFS8 4728
Proximity Label-MS Homo sapiens
166 MRPS18B 28973
Proximity Label-MS Homo sapiens
167 NDUFA2 4695
Proximity Label-MS Homo sapiens
168 MRPL13  
Proximity Label-MS Homo sapiens
169 MRPS33  
Proximity Label-MS Homo sapiens
170 MRPL46  
Proximity Label-MS Homo sapiens
171 MRPL19 9801
Proximity Label-MS Homo sapiens
172 COX5A 9377
Proximity Label-MS Homo sapiens
173 MRPS16  
Proximity Label-MS Homo sapiens
174 GRSF1 2926
Proximity Label-MS Homo sapiens
175 C1QBP 708
Affinity Capture-MS Homo sapiens
176 NDUFAF4 29078
Proximity Label-MS Homo sapiens
177 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
178 MRPL53  
Proximity Label-MS Homo sapiens
179 MRPS24 64951
Proximity Label-MS Homo sapiens
180 MRPS22 56945
Proximity Label-MS Homo sapiens
181 DNMT1 1786
Affinity Capture-MS Homo sapiens
182 MRPL28 10573
Proximity Label-MS Homo sapiens
183 NDUFS6  
Proximity Label-MS Homo sapiens
184 MRPS9 64965
Proximity Label-MS Homo sapiens
185 TIMM44 10469
Proximity Label-MS Homo sapiens
186 ECSIT 51295
Proximity Label-MS Homo sapiens
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