Gene description for PTCD3
Gene name pentatricopeptide repeat domain 3
Gene symbol PTCD3
Other names/aliases MRP-S39
Species Homo sapiens
 Database cross references - PTCD3
ExoCarta ExoCarta_55037
Entrez Gene 55037
HGNC 24717
MIM 614918
UniProt Q96EY7  
 PTCD3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PTCD3
Molecular Function
    rRNA binding GO:0019843 IDA
    poly(A) RNA binding GO:0044822 IDA
    ribosomal small subunit binding GO:0043024 IDA
Biological Process
    regulation of translation GO:0006417 IEA
    mitochondrial translational initiation GO:0070124 TAS
    mitochondrial translation GO:0032543 TAS
    organelle organization GO:0006996 TAS
    mitochondrial translational termination GO:0070126 TAS
    mitochondrial translational elongation GO:0070125 TAS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified PTCD3 in exosomes
1
Experiment ID 234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PTCD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RFXANK  
Affinity Capture-MS Homo sapiens
2 ATF4  
Two-hybrid Homo sapiens
3 LUC7L2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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