Gene description for GFM2
Gene name G elongation factor, mitochondrial 2
Gene symbol GFM2
Other names/aliases EF-G2mt
EFG2
MRRF2
MST027
MSTP027
RRF2
RRF2mt
hEFG2
mEF-G 2
Species Homo sapiens
 Database cross references - GFM2
ExoCarta ExoCarta_84340
Vesiclepedia VP_84340
Entrez Gene 84340
HGNC 29682
MIM 606544
UniProt Q969S9  
 GFM2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for GFM2
Molecular Function
    translation elongation factor activity GO:0003746 IDA
    GTPase activity GO:0003924 EXP
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
Biological Process
    mitochondrial translation GO:0032543 IBA
    mitochondrial translation GO:0032543 IDA
    ribosome disassembly GO:0032790 IBA
    ribosome disassembly GO:0032790 IDA
    mitochondrial translational elongation GO:0070125 IDA
    mitochondrial translational termination GO:0070126 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified GFM2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GFM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPS27 23107
Proximity Label-MS Homo sapiens
2 AFG3L2 10939
Proximity Label-MS Homo sapiens
3 MRPS35 60488
Proximity Label-MS Homo sapiens
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 LCN8  
Affinity Capture-MS Homo sapiens
6 ATP5J2-PTCD1  
Proximity Label-MS Homo sapiens
7 SLIRP 81892
Proximity Label-MS Homo sapiens
8 NDUFS1 4719
Proximity Label-MS Homo sapiens
9 MRPL17  
Proximity Label-MS Homo sapiens
10 SQSTM1 8878
Affinity Capture-MS Homo sapiens
11 PDK3 5165
Proximity Label-MS Homo sapiens
12 MRPL24  
Proximity Label-MS Homo sapiens
13 MRPL37 51253
Proximity Label-MS Homo sapiens
14 ETFB 2109
Proximity Label-MS Homo sapiens
15 NDUFA12 55967
Proximity Label-MS Homo sapiens
16 MRPL50 54534
Proximity Label-MS Homo sapiens
17 CALM1 801
Affinity Capture-MS Homo sapiens
18 MRPL52  
Proximity Label-MS Homo sapiens
19 MRPL49 740
Proximity Label-MS Homo sapiens
20 NDUFS2 4720
Proximity Label-MS Homo sapiens
21 LRPPRC 10128
Proximity Label-MS Homo sapiens
22 ATP5D 513
Proximity Label-MS Homo sapiens
23 MRPL20 55052
Proximity Label-MS Homo sapiens
24 NDUFS3 4722
Proximity Label-MS Homo sapiens
25 OGDH 4967
Proximity Label-MS Homo sapiens
26 IARS2 55699
Proximity Label-MS Homo sapiens
27 NDUFA7 4701
Proximity Label-MS Homo sapiens
28 MRPS5 64969
Proximity Label-MS Homo sapiens
29 YARS2  
Affinity Capture-MS Homo sapiens
30 HINT2 84681
Proximity Label-MS Homo sapiens
31 MRPS23 51649
Proximity Label-MS Homo sapiens
32 MRPS24 64951
Proximity Label-MS Homo sapiens
33 MRPL12 6182
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ETFA 2108
Proximity Label-MS Homo sapiens
35 MRPS26 64949
Proximity Label-MS Homo sapiens
36 MALSU1  
Affinity Capture-MS Homo sapiens
37 ACOT2 10965
Proximity Label-MS Homo sapiens
38 ATP5O 539
Proximity Label-MS Homo sapiens
39 PSG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ERAL1  
Proximity Label-MS Homo sapiens
41 MRPL39 54148
Proximity Label-MS Homo sapiens
42 NDUFV1 4723
Proximity Label-MS Homo sapiens
43 NDUFV3 4731
Proximity Label-MS Homo sapiens
44 ATP5H 10476
Proximity Label-MS Homo sapiens
45 SLC30A9 10463
Proximity Label-MS Homo sapiens
46 BCS1L 617
Proximity Label-MS Homo sapiens
47 TMEM132D  
Two-hybrid Homo sapiens
48 FASTKD2  
Proximity Label-MS Homo sapiens
49 NME4 4833
Proximity Label-MS Homo sapiens
50 NDUFA6  
Proximity Label-MS Homo sapiens
51 PDPR 55066
Proximity Label-MS Homo sapiens
52 MRPL47 57129
Proximity Label-MS Homo sapiens
53 MTERF3  
Proximity Label-MS Homo sapiens
54 RBMX 27316
Proximity Label-MS Homo sapiens
55 STOML2 30968
Proximity Label-MS Homo sapiens
56 MRPS30 10884
Proximity Label-MS Homo sapiens
57 ALAS1  
Proximity Label-MS Homo sapiens
58 TRIM41 90933
Affinity Capture-MS Homo sapiens
59 BANP  
Two-hybrid Homo sapiens
60 MRPS25 64432
Proximity Label-MS Homo sapiens
61 MRPS33  
Proximity Label-MS Homo sapiens
62 NDUFS4 4724
Proximity Label-MS Homo sapiens
63 PTCD1 26024
Affinity Capture-MS Homo sapiens
64 MRPL45 84311
Proximity Label-MS Homo sapiens
65 PARK2  
Affinity Capture-MS Homo sapiens
66 MRPS34 65993
Proximity Label-MS Homo sapiens
67 BEX4  
Affinity Capture-MS Homo sapiens
68 ATPAF1  
Proximity Label-MS Homo sapiens
69 TUFM 7284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 GTPBP10  
Proximity Label-MS Homo sapiens
71 PTCD3 55037
Proximity Label-MS Homo sapiens
72 NDUFS8 4728
Proximity Label-MS Homo sapiens
73 MMAB 326625
Proximity Label-MS Homo sapiens
74 BCL2L12 83596
Affinity Capture-MS Homo sapiens
75 MRPS6  
Proximity Label-MS Homo sapiens
76 POLDIP2 26073
Proximity Label-MS Homo sapiens
77 C10orf2 56652
Proximity Label-MS Homo sapiens
78 DBT 1629
Proximity Label-MS Homo sapiens
79 ATP5B 506
Proximity Label-MS Homo sapiens
80 MRPL2 51069
Co-fractionation Homo sapiens
81 SSX2IP  
Proximity Label-MS Homo sapiens
82 MRPL41 64975
Proximity Label-MS Homo sapiens
83 OXA1L  
Proximity Label-MS Homo sapiens
84 MRPL44  
Proximity Label-MS Homo sapiens
85 RARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 MRPL21 219927
Proximity Label-MS Homo sapiens
87 IBA57  
Proximity Label-MS Homo sapiens
88 MRPL10 124995
Proximity Label-MS Homo sapiens
89 DAP3 7818
Proximity Label-MS Homo sapiens
90 TRMT10C 54931
Proximity Label-MS Homo sapiens
91 PNPT1 87178
Proximity Label-MS Homo sapiens
92 Cct3 12462
Affinity Capture-MS Mus musculus
93 NDUFA9 4704
Proximity Label-MS Homo sapiens
94 LETM1 3954
Proximity Label-MS Homo sapiens
95 NPTN 27020
Affinity Capture-MS Homo sapiens
96 MRPS7 51081
Proximity Label-MS Homo sapiens
97 MRPL9 65005
Proximity Label-MS Homo sapiens
98 MRPS14  
Proximity Label-MS Homo sapiens
99 NFS1 9054
Proximity Label-MS Homo sapiens
100 ATP5F1 515
Proximity Label-MS Homo sapiens
101 GLS 2744
Proximity Label-MS Homo sapiens
102 PYCR1 5831
Proximity Label-MS Homo sapiens
103 FAHD1 81889
Affinity Capture-MS Homo sapiens
104 ELAC2 60528
Proximity Label-MS Homo sapiens
105 GADD45GIP1  
Proximity Label-MS Homo sapiens
106 DHX30 22907
Proximity Label-MS Homo sapiens
107 PYCR2 29920
Proximity Label-MS Homo sapiens
108 BPNT1 10380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
110 GLUD1 2746
Proximity Label-MS Homo sapiens
111 MRPL15 29088
Proximity Label-MS Homo sapiens
112 MRPL4 51073
Proximity Label-MS Homo sapiens
113 TACO1  
Proximity Label-MS Homo sapiens
114 DLST 1743
Proximity Label-MS Homo sapiens
115 TRIM27  
Two-hybrid Homo sapiens
116 TRA2A 29896
Proximity Label-MS Homo sapiens
117 MRPS36 92259
Proximity Label-MS Homo sapiens
118 VWA8 23078
Proximity Label-MS Homo sapiens
119 MRPS17 51373
Proximity Label-MS Homo sapiens
120 NDUFV2 4729
Proximity Label-MS Homo sapiens
121 ECHS1 1892
Proximity Label-MS Homo sapiens
122 PPIF 10105
Proximity Label-MS Homo sapiens
123 MRPL42  
Proximity Label-MS Homo sapiens
124 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
125 NDUFAF2  
Proximity Label-MS Homo sapiens
126 POLG  
Proximity Label-MS Homo sapiens
127 ATP5J 522
Proximity Label-MS Homo sapiens
128 MRPS2 51116
Proximity Label-MS Homo sapiens
129 COQ5  
Proximity Label-MS Homo sapiens
130 VPS52 6293
Two-hybrid Homo sapiens
131 MRPS28  
Proximity Label-MS Homo sapiens
132 LARS2 23395
Proximity Label-MS Homo sapiens
133 SRSF9 8683
Proximity Label-MS Homo sapiens
134 MRPL53  
Proximity Label-MS Homo sapiens
135 MRPL43 84545
Proximity Label-MS Homo sapiens
136 PDHA1 5160
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
137 ATP5L2  
Affinity Capture-MS Homo sapiens
138 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
139 TRIM9  
Two-hybrid Homo sapiens
140 MRPS10 55173
Proximity Label-MS Homo sapiens
141 SHMT2 6472
Proximity Label-MS Homo sapiens
142 TRA2B 6434
Proximity Label-MS Homo sapiens
143 RPS5 6193
Co-fractionation Homo sapiens
144 NDUFA5 4698
Proximity Label-MS Homo sapiens
145 MRPS18B 28973
Proximity Label-MS Homo sapiens
146 NDUFA2 4695
Proximity Label-MS Homo sapiens
147 MRPL13  
Proximity Label-MS Homo sapiens
148 ACOT1 641371
Proximity Label-MS Homo sapiens
149 MRPL46  
Proximity Label-MS Homo sapiens
150 MRPL19 9801
Proximity Label-MS Homo sapiens
151 TIMM44 10469
Proximity Label-MS Homo sapiens
152 MRPS16  
Proximity Label-MS Homo sapiens
153 GRSF1 2926
Proximity Label-MS Homo sapiens
154 NDUFAF4 29078
Proximity Label-MS Homo sapiens
155 KRAS 3845
Negative Genetic Homo sapiens
156 MRPS31  
Proximity Label-MS Homo sapiens
157 C17orf80 55028
Proximity Label-MS Homo sapiens
158 MRPS22 56945
Proximity Label-MS Homo sapiens
159 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 PLD6  
Affinity Capture-MS Homo sapiens
161 MRPL28 10573
Proximity Label-MS Homo sapiens
162 NDUFS6  
Proximity Label-MS Homo sapiens
163 MRPS9 64965
Proximity Label-MS Homo sapiens
164 RPL17 6139
Co-fractionation Homo sapiens
View the network image/svg+xml



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