Gene description for ACOT1
Gene name acyl-CoA thioesterase 1
Gene symbol ACOT1
Other names/aliases ACH2
CTE-1
LACH2
Species Homo sapiens
 Database cross references - ACOT1
ExoCarta ExoCarta_641371
Entrez Gene 641371
HGNC 33128
MIM 614313
UniProt Q86TX2  
 ACOT1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ACOT1
Molecular Function
    palmitoyl-CoA hydrolase activity GO:0016290 IEA
    carboxylic ester hydrolase activity GO:0052689 IEA
    acyl-CoA hydrolase activity GO:0047617 IDA
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IDA
    acyl-CoA metabolic process GO:0006637 IDA
    long-chain fatty acid metabolic process GO:0001676 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ACOT1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACOT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ACOT1 is involved
No pathways found





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