Gene description for ACOT1
Gene name acyl-CoA thioesterase 1
Gene symbol ACOT1
Other names/aliases ACH2
CTE-1
LACH2
Species Homo sapiens
 Database cross references - ACOT1
ExoCarta ExoCarta_641371
Vesiclepedia VP_641371
Entrez Gene 641371
HGNC 33128
MIM 614313
UniProt Q86TX2  
 ACOT1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ACOT1
Molecular Function
    protein binding GO:0005515 IPI
    fatty acyl-CoA hydrolase activity GO:0047617 IBA
    fatty acyl-CoA hydrolase activity GO:0047617 IDA
    carboxylic ester hydrolase activity GO:0052689 IEA
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IDA
    long-chain fatty acid metabolic process GO:0001676 IDA
    fatty acid metabolic process GO:0006631 IBA
    acyl-CoA metabolic process GO:0006637 IBA
    acyl-CoA metabolic process GO:0006637 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ACOT1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACOT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLIRP 81892
Proximity Label-MS Homo sapiens
2 TRUB2  
Proximity Label-MS Homo sapiens
3 ACTC1 70
Proximity Label-MS Homo sapiens
4 Chmp4b 75608
Affinity Capture-MS Mus musculus
5 MTG2  
Proximity Label-MS Homo sapiens
6 NGRN  
Proximity Label-MS Homo sapiens
7 CHCHD1  
Proximity Label-MS Homo sapiens
8 CNP 1267
Affinity Capture-MS Homo sapiens
9 PPIA 5478
Co-fractionation Homo sapiens
10 RPUSD4 84881
Proximity Label-MS Homo sapiens
11 CFL1 1072
Co-fractionation Homo sapiens
12 PARK7 11315
Co-fractionation Homo sapiens
13 MTRF1  
Proximity Label-MS Homo sapiens
14 GFM2 84340
Proximity Label-MS Homo sapiens
15 CCDC90B  
Proximity Label-MS Homo sapiens
16 SPG7  
Affinity Capture-MS Homo sapiens
17 LRPPRC 10128
Proximity Label-MS Homo sapiens
18 MCU 90550
Proximity Label-MS Homo sapiens
19 CRYZ 1429
Proximity Label-MS Homo sapiens
20 TFAM 7019
Proximity Label-MS Homo sapiens
21 PMPCA 23203
Proximity Label-MS Homo sapiens
22 C8orf82  
Proximity Label-MS Homo sapiens
23 HINT2 84681
Proximity Label-MS Homo sapiens
24 HIBCH 26275
Co-fractionation Homo sapiens
25 MRPS26 64949
Proximity Label-MS Homo sapiens
26 SURF1  
Proximity Label-MS Homo sapiens
27 EXD2  
Proximity Label-MS Homo sapiens
28 PMPCB 9512
Proximity Label-MS Homo sapiens
29 TACO1  
Proximity Label-MS Homo sapiens
30 FASTKD2  
Proximity Label-MS Homo sapiens
31 VWA8 23078
Proximity Label-MS Homo sapiens
32 MTERF3  
Proximity Label-MS Homo sapiens
33 MDH2 4191
Proximity Label-MS Homo sapiens
34 TFAP2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HHATL  
Affinity Capture-MS Homo sapiens
36 C6orf203  
Proximity Label-MS Homo sapiens
37 COX15 1355
Proximity Label-MS Homo sapiens
38 FASTKD5  
Proximity Label-MS Homo sapiens
39 HINT1 3094
Co-fractionation Homo sapiens
40 CXADR 1525
Co-fractionation Homo sapiens
41 ACOT2 10965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TUFM 7284
Proximity Label-MS Homo sapiens
43 HSCB 150274
Proximity Label-MS Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 CCDC109B 55013
Proximity Label-MS Homo sapiens
46 TMPRSS11E  
Affinity Capture-MS Homo sapiens
47 GFM1 85476
Proximity Label-MS Homo sapiens
48 MTFMT  
Proximity Label-MS Homo sapiens
49 MRPL11 65003
Proximity Label-MS Homo sapiens
50 MTRF1L  
Proximity Label-MS Homo sapiens
51 TMEM70  
Proximity Label-MS Homo sapiens
52 FAHD2A 51011
Co-fractionation Homo sapiens
53 COX4I1 1327
Proximity Label-MS Homo sapiens
54 TXN2 25828
Co-fractionation Homo sapiens
55 OTC 5009
Proximity Label-MS Homo sapiens
56 WBSCR16  
Proximity Label-MS Homo sapiens
57 MRRF  
Proximity Label-MS Homo sapiens
58 AIFM1 9131
Proximity Label-MS Homo sapiens
59 MRPS12  
Proximity Label-MS Homo sapiens
60 MTIF2 4528
Proximity Label-MS Homo sapiens
61 MTIF3  
Proximity Label-MS Homo sapiens
62 SLC35A5  
Affinity Capture-MS Homo sapiens
63 TST 7263
Co-fractionation Homo sapiens
64 DHX30 22907
Proximity Label-MS Homo sapiens
65 C12orf65  
Proximity Label-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 CA2 760
Co-fractionation Homo sapiens
68 ACAD9 28976
Proximity Label-MS Homo sapiens
69 PATZ1  
Affinity Capture-MS Homo sapiens
70 HSPA9 3313
Proximity Label-MS Homo sapiens
71 METTL15  
Proximity Label-MS Homo sapiens
72 MCUR1 63933
Proximity Label-MS Homo sapiens
73 CLPP 8192
Proximity Label-MS Homo sapiens
74 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
75 C17orf80 55028
Proximity Label-MS Homo sapiens
76 TSFM 10102
Proximity Label-MS Homo sapiens
77 AUH 549
Proximity Label-MS Homo sapiens
78 DNAJC19 131118
Proximity Label-MS Homo sapiens
79 ICT1 3396
Proximity Label-MS Homo sapiens
80 LONP1 9361
Proximity Label-MS Homo sapiens
81 LGALS3 3958
Co-fractionation Homo sapiens
82 ACAA2 10449
Co-fractionation Homo sapiens
83 PDHA1 5160
Proximity Label-MS Homo sapiens
84 DNAJA3 9093
Proximity Label-MS Homo sapiens
85 COX8A  
Proximity Label-MS Homo sapiens
86 NUDT19 390916
Co-fractionation Homo sapiens
87 C21orf33  
Proximity Label-MS Homo sapiens
88 SSBP1 6742
Proximity Label-MS Homo sapiens
89 PCNA 5111
Affinity Capture-MS Homo sapiens
90 FASTKD3  
Proximity Label-MS Homo sapiens
91 TBRG4 9238
Proximity Label-MS Homo sapiens
92 AARS2  
Proximity Label-MS Homo sapiens
93 KCNA10  
Affinity Capture-MS Homo sapiens
94 TEFM  
Proximity Label-MS Homo sapiens
95 RPUSD3  
Proximity Label-MS Homo sapiens
96 GRSF1 2926
Proximity Label-MS Homo sapiens
97 C1QBP 708
Proximity Label-MS Homo sapiens
98 METTL17  
Proximity Label-MS Homo sapiens
99 AK1 203
Co-fractionation Homo sapiens
100 ZFP69B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ACOT1 is involved
PathwayEvidenceSource
Fatty acid metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Mitochondrial Fatty Acid Beta-Oxidation TAS Reactome





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