Gene description for Chmp4b
Gene name charged multivesicular body protein 4B
Gene symbol Chmp4b
Other names/aliases 2010012F05Rik
C76846
Snf7-2
Species Mus musculus
 Database cross references - Chmp4b
ExoCarta ExoCarta_75608
Vesiclepedia VP_75608
Entrez Gene 75608
UniProt Q9D8B3  
 Chmp4b identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Chmp4b
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
Biological Process
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    plasma membrane repair GO:0001778 ISO
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 IEA
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 ISO
    vesicle budding from membrane GO:0006900 IBA
    autophagy GO:0006914 ISO
    nucleus organization GO:0006997 ISO
    mitotic metaphase chromosome alignment GO:0007080 ISO
    exit from mitosis GO:0010458 ISO
    exit from mitosis GO:0010458 ISS
    regulation of autophagy GO:0010506 IMP
    regulation of centrosome duplication GO:0010824 IEA
    regulation of centrosome duplication GO:0010824 ISO
    protein transport GO:0015031 IEA
    nuclear membrane reassembly GO:0031468 ISO
    nuclear membrane reassembly GO:0031468 ISS
    late endosome to vacuole transport via multivesicular body sorting pathway GO:0032511 IBA
    multivesicular body assembly GO:0036258 NAS
    maintenance of lens transparency GO:0036438 IEA
    maintenance of lens transparency GO:0036438 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    viral budding GO:0046755 ISO
    viral budding from plasma membrane GO:0046761 ISO
    nervous system process GO:0050877 IEA
    nervous system process GO:0050877 ISO
    protein polymerization GO:0051258 IEA
    protein polymerization GO:0051258 ISO
    vesicle fusion with vacuole GO:0051469 NAS
    multivesicular body-lysosome fusion GO:0061763 NAS
    midbody abscission GO:0061952 ISO
    multivesicular body sorting pathway GO:0071985 ISO
    membrane fission GO:0090148 ISO
    membrane fission GO:0090148 ISS
    membrane fission GO:0090148 NAS
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 IEA
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    autophagosome maturation GO:0097352 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    late endosome to lysosome transport GO:1902774 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 ISO
    kinetochore GO:0000776 ISO
    ESCRT III complex GO:0000815 IBA
    ESCRT III complex GO:0000815 ISO
    ESCRT III complex GO:0000815 ISS
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    nuclear pore GO:0005643 ISO
    cytoplasm GO:0005737 ISO
    lysosomal membrane GO:0005765 ISO
    endosome GO:0005768 ISO
    multivesicular body GO:0005771 IBA
    kinetochore microtubule GO:0005828 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 ISO
    cytoplasmic side of plasma membrane GO:0009898 IBA
    cytoplasmic side of plasma membrane GO:0009898 IEA
    cytoplasmic side of plasma membrane GO:0009898 ISO
    endosome membrane GO:0010008 IDA
    postsynaptic density GO:0014069 ISO
    membrane GO:0016020 IDA
    membrane coat GO:0030117 IEA
    membrane coat GO:0030117 ISO
    midbody GO:0030496 ISO
    vesicle GO:0031982 ISO
    multivesicular body membrane GO:0032585 ISO
    glutamatergic synapse GO:0098978 ISO
    amphisome membrane GO:1904930 ISO
 Experiment description of studies that identified Chmp4b in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Chmp4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHMP2A 27243
Affinity Capture-MS Homo sapiens
2 ABCG2 9429
Affinity Capture-MS Homo sapiens
3 MVP 9961
Affinity Capture-MS Homo sapiens
4 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
5 Usp8  
Co-fractionation Mus musculus
6 POLDIP3 84271
Affinity Capture-MS Homo sapiens
7 MITD1 129531
Affinity Capture-MS Homo sapiens
8 CPM 1368
Affinity Capture-MS Homo sapiens
9 RACGAP1 29127
Affinity Capture-MS Homo sapiens
10 THOC2 57187
Affinity Capture-MS Homo sapiens
11 CD2AP 23607
Affinity Capture-MS Homo sapiens
12 EFTUD1 79631
Affinity Capture-MS Homo sapiens
13 CHMP3 51652
Affinity Capture-MS Homo sapiens
14 RPUSD4 84881
Affinity Capture-MS Homo sapiens
15 UAP1 6675
Affinity Capture-MS Homo sapiens
16 Tmem173  
Proximity Label-MS Mus musculus
17 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
18 CHAF1A  
Affinity Capture-MS Homo sapiens
19 MDN1 23195
Affinity Capture-MS Homo sapiens
20 CHMP5 51510
Affinity Capture-MS Homo sapiens
21 TFRC 7037
Affinity Capture-MS Homo sapiens
22 ABCD3 5825
Affinity Capture-MS Homo sapiens
23 CHMP2B 25978
Affinity Capture-MS Homo sapiens
24 CHMP1B 57132
Affinity Capture-MS Homo sapiens
25 CHMP4A 29082
Affinity Capture-MS Homo sapiens
26 TPRN 286262
Affinity Capture-MS Homo sapiens
27 CCDC115 84317
Affinity Capture-MS Homo sapiens
28 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
29 PKN3 29941
Affinity Capture-MS Homo sapiens
30 SDCBP 6386
Affinity Capture-MS Homo sapiens
31 ATP6AP1 537
Affinity Capture-MS Homo sapiens
32 CYBRD1 79901
Affinity Capture-MS Homo sapiens
33 MICA 100507436
Affinity Capture-MS Homo sapiens
34 STOML2 30968
Affinity Capture-MS Homo sapiens
35 CC2D1B 200014
Affinity Capture-MS Homo sapiens
36 CHMP1A 5119
Affinity Capture-MS Homo sapiens
37 BAZ2B  
Affinity Capture-MS Homo sapiens
38 STOM 2040
Affinity Capture-MS Homo sapiens
39 BDP1  
Affinity Capture-MS Homo sapiens
40 USP8 9101
Affinity Capture-MS Homo sapiens
41 GNAI3 2773
Affinity Capture-MS Homo sapiens
42 PABPN1 8106
Affinity Capture-MS Homo sapiens
43 Traf3  
Proximity Label-MS Mus musculus
44 DDX39B 7919
Affinity Capture-MS Homo sapiens
45 FLOT2 2319
Affinity Capture-MS Homo sapiens
46 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
47 GPRC5A 9052
Affinity Capture-MS Homo sapiens
48 Chmp1a  
Co-fractionation Mus musculus
49 RBBP6 5930
Affinity Capture-MS Homo sapiens
50 FNIP1  
Affinity Capture-MS Homo sapiens
51 MAP7 9053
Affinity Capture-MS Homo sapiens
52 VPS4B 9525
Affinity Capture-MS Homo sapiens
53 NHSL1  
Affinity Capture-MS Homo sapiens
54 PACSIN3 29763
Affinity Capture-MS Homo sapiens
55 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
56 Vps4b 20479
Co-fractionation Mus musculus
57 YTHDC1  
Affinity Capture-MS Homo sapiens
58 SOAT1 6646
Affinity Capture-MS Homo sapiens
59 IST1 9798
Affinity Capture-MS Homo sapiens
60 CRTC3  
Affinity Capture-MS Homo sapiens
61 OBSCN 84033
Affinity Capture-MS Homo sapiens
62 BAZ1A 11177
Affinity Capture-MS Homo sapiens
63 PTPN23 25930
Affinity Capture-MS Homo sapiens
64 KTN1 3895
Affinity Capture-MS Homo sapiens
65 Ndn  
Two-hybrid Mus musculus
66 KIDINS220 57498
Affinity Capture-MS Homo sapiens
67 INTS5 80789
Affinity Capture-MS Homo sapiens
68 FLOT1 10211
Affinity Capture-MS Homo sapiens
69 MVB12A 93343
Affinity Capture-MS Homo sapiens
70 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
71 PHB2 11331
Affinity Capture-MS Homo sapiens
72 SLC38A2 54407
Affinity Capture-MS Homo sapiens
73 CHAF1B  
Affinity Capture-MS Homo sapiens
74 JUP 3728
Affinity Capture-MS Homo sapiens
75 RIMS1  
Affinity Capture-MS Homo sapiens
76 ERLIN1 10613
Affinity Capture-MS Homo sapiens
77 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
78 2610002M06Rik  
Co-fractionation Mus musculus
79 BST2 684
Affinity Capture-MS Homo sapiens
80 PDCD6 10016
Affinity Capture-MS Homo sapiens
81 NELFA  
Affinity Capture-MS Homo sapiens
82 RNF20 56254
Affinity Capture-MS Homo sapiens
83 IPP  
Affinity Capture-MS Homo sapiens
84 DPH7  
Affinity Capture-MS Homo sapiens
85 ACOT1 641371
Affinity Capture-MS Homo sapiens
86 XPR1 9213
Affinity Capture-MS Homo sapiens
87 CC2D1A 54862
Affinity Capture-MS Homo sapiens
88 CKAP4 10970
Affinity Capture-MS Homo sapiens
89 PTRF 284119
Affinity Capture-MS Homo sapiens
90 CAV1 857
Affinity Capture-MS Homo sapiens
91 DSG2 1829
Affinity Capture-MS Homo sapiens
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