Gene description for Chmp4b
Gene name charged multivesicular body protein 4B
Gene symbol Chmp4b
Other names/aliases 2010012F05Rik
C76846
Snf7-2
Species Mus musculus
 Database cross references - Chmp4b
ExoCarta ExoCarta_75608
Entrez Gene 75608
UniProt Q9D8B3  
 Chmp4b identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Chmp4b
Molecular Function
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
Biological Process
    nucleus organization GO:0006997 ISO
    cell separation after cytokinesis GO:0000920 ISO
    mitotic metaphase plate congression GO:0007080 ISO
    regulation of centrosome duplication GO:0010824 ISO
    negative regulation of cell death GO:0060548 ISO
    negative regulation of autophagosome assembly GO:1902902 ISO
    viral budding GO:0046755 ISO
    maintenance of lens transparency GO:0036438 ISO
    protein homooligomerization GO:0051260 ISO
    regulation of viral process GO:0050792 ISO
    protein transport GO:0015031 IEA
    regulation of mitotic spindle assembly GO:1901673 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    positive regulation of viral release from host cell GO:1902188 ISO
    vacuolar transport GO:0007034 IEA
    transport GO:0006810 IEA
    negative regulation of neuron death GO:1901215 ISO
    posttranslational protein targeting to membrane GO:0006620 ISO
Subcellular Localization
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    midbody GO:0030496 ISO
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    nucleus GO:0005634 ISO
    ESCRT III complex GO:0000815 ISO
    membrane coat GO:0030117 ISO
 Experiment description of studies that identified Chmp4b in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Chmp4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Chmp4b is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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