Gene description for USP8
Gene name ubiquitin specific peptidase 8
Gene symbol USP8
Other names/aliases HumORF8
SPG59
UBPY
Species Homo sapiens
 Database cross references - USP8
ExoCarta ExoCarta_9101
Vesiclepedia VP_9101
Entrez Gene 9101
HGNC 12631
MIM 603158
UniProt P40818  
 USP8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for USP8
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 TAS
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 TAS
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    cadherin binding GO:0045296 HDA
    K63-linked deubiquitinase activity GO:0061578 IDA
    K48-linked deubiquitinase activity GO:1990380 IDA
Biological Process
    mitotic cytokinesis GO:0000281 IMP
    proteolysis GO:0006508 IEA
    endosome organization GO:0007032 IBA
    Ras protein signal transduction GO:0007265 IBA
    protein deubiquitination GO:0016579 IMP
    protein deubiquitination GO:0016579 IMP
    regulation of protein stability GO:0031647 IMP
    regulation of protein localization GO:0032880 IMP
    protein K63-linked deubiquitination GO:0070536 IDA
    protein K48-linked deubiquitination GO:0071108 IDA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    regulation of protein catabolic process at postsynapse, modulating synaptic transmission GO:0099576 IEA
    negative regulation of lysosomal protein catabolic process GO:1905166 IMP
    positive regulation of amyloid fibril formation GO:1905908 IMP
    cellular response to nerve growth factor stimulus GO:1990090 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    endosome membrane GO:0010008 IEA
    postsynaptic density GO:0014069 IBA
    midbody GO:0030496 IBA
    midbody GO:0030496 IDA
    dendritic spine GO:0043197 IBA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified USP8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for USP8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 CHMP2A 27243
Reconstituted Complex Homo sapiens
3 GRAP2 9402
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 PPP2R4 5524
Affinity Capture-Western Homo sapiens
7 SCNN1A  
Affinity Capture-Western Homo sapiens
8 CHMP1A 5119
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 TARDBP 23435
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 Chmp4b 75608
Affinity Capture-MS Mus musculus
13 EPS15 2060
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
14 UBC 7316
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
15 TRIM55  
Biochemical Activity Homo sapiens
16 CBY1  
Affinity Capture-MS Homo sapiens
17 TRIM54  
Biochemical Activity Homo sapiens
18 PJA1  
Biochemical Activity Homo sapiens
19 MAP3K7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 KIF23 9493
Affinity Capture-Western Homo sapiens
21 RAB3IP  
Affinity Capture-MS Homo sapiens
22 SRC 6714
Biochemical Activity Homo sapiens
23 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SCNN1B 6338
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 CHMP2B 25978
Reconstituted Complex Homo sapiens
26 NR3C1 2908
Proximity Label-MS Homo sapiens
27 KRT31 3881
Affinity Capture-MS Homo sapiens
28 CHMP1B 57132
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
29 STX6 10228
Proximity Label-MS Homo sapiens
30 TATDN1 83940
Co-fractionation Homo sapiens
31 VPS4B 9525
Co-fractionation Homo sapiens
32 YWHAH 7533
Affinity Capture-MS Homo sapiens
33 SFN 2810
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
34 STAT6 6778
Co-fractionation Homo sapiens
35 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
36 EEA1 8411
Co-localization Homo sapiens
37 KCNE3  
Affinity Capture-MS Homo sapiens
38 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 USP22 23326
Affinity Capture-MS Homo sapiens
40 CHMP4C 92421
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
41 MARCH1  
Affinity Capture-MS Homo sapiens
42 STAM2 10254
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
PCA Homo sapiens
43 PPP1CA 5499
Affinity Capture-Western Homo sapiens
44 CHMP7 91782
Reconstituted Complex Homo sapiens
45 USP21  
Biochemical Activity Homo sapiens
46 ERBB3 2065
Affinity Capture-Western Homo sapiens
47 NBR1 4077
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
48 USP8 9101
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
49 HTT 3064
Co-fractionation Homo sapiens
50 UBE2I 7329
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
51 CDH1 999
Proximity Label-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 HIST2H2AC 8338
Biochemical Activity Homo sapiens
54 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
55 EPG5 57724
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
56 USP28 57646
Biochemical Activity Homo sapiens
57 TRIM63  
Biochemical Activity Homo sapiens
58 HIST2H2BE 8349
Biochemical Activity Homo sapiens
59 PTAR1 375743
Synthetic Lethality Homo sapiens
60 RPL6P1  
Affinity Capture-MS Homo sapiens
61 KRT85  
Affinity Capture-MS Homo sapiens
62 SCNN1G  
Affinity Capture-Western Homo sapiens
63 Cd2ap 12488
Affinity Capture-MS Mus musculus
64 VAMP8 8673
Affinity Capture-Western Homo sapiens
65 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 USP15 9958
Biochemical Activity Homo sapiens
67 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 RNF128  
Affinity Capture-Western Homo sapiens
69 POLD3  
Co-fractionation Homo sapiens
70 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 COMMD2 51122
Co-fractionation Homo sapiens
72 PTEN 5728
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
73 ERBB4 2066
Affinity Capture-Western Homo sapiens
74 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Negative Genetic Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
75 Ywhae 22627
Affinity Capture-Western Mus musculus
76 ACTRT1  
Affinity Capture-MS Homo sapiens
77 BIRC6 57448
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
78 IST1 9798
Affinity Capture-MS Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 LRIG1 26018
Affinity Capture-Western Homo sapiens
81 UBE2E1  
Co-fractionation Homo sapiens
82 TP63  
Affinity Capture-Western Homo sapiens
83 FZD5 7855
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
84 KCNN4 3783
Affinity Capture-Western Homo sapiens
85 PTGR2 145482
Co-fractionation Homo sapiens
86 PLA2G2A 5320
Biochemical Activity Homo sapiens
87 ARID1A 8289
Negative Genetic Homo sapiens
88 TPCN2  
Affinity Capture-MS Homo sapiens
89 AKT1 207
Affinity Capture-Western Homo sapiens
90 DSP 1832
Cross-Linking-MS (XL-MS) Homo sapiens
91 Rasgrf1  
Affinity Capture-Western Mus musculus
92 SNCA 6622
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
Biochemical Activity Homo sapiens
93 GRB2 2885
Reconstituted Complex Homo sapiens
94 KRT36 8689
Affinity Capture-MS Homo sapiens
95 C10orf76  
Synthetic Lethality Homo sapiens
96 BACE1  
Biochemical Activity Homo sapiens
97 SPP1 6696
Two-hybrid Homo sapiens
98 ACAA2 10449
Co-fractionation Homo sapiens
99 TSEN2  
Co-fractionation Homo sapiens
100 OTUB1 55611
Affinity Capture-Western Homo sapiens
101 FARSB 10056
Co-fractionation Homo sapiens
102 MCPH1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
103 TMEM79  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
104 EPHA2 1969
Proximity Label-MS Homo sapiens
105 KRAS 3845
Negative Genetic Homo sapiens
106 UBE2G1 7326
Co-fractionation Homo sapiens
107 STAM 8027
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
108 Rnf41  
Affinity Capture-Western Mus musculus
109 RNF41  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml



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