Gene description for GRAP2
Gene name GRB2-related adaptor protein 2
Gene symbol GRAP2
Other names/aliases GADS
GRAP-2
GRB2L
GRBLG
GRID
GRPL
GrbX
Grf40
Mona
P38
Species Homo sapiens
 Database cross references - GRAP2
ExoCarta ExoCarta_9402
Entrez Gene 9402
HGNC 4563
MIM 604518
UniProt O75791  
 GRAP2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for GRAP2
Molecular Function
    protein binding GO:0005515 IPI
    SH3/SH2 adaptor activity GO:0005070 TAS
Biological Process
    cell-cell signaling GO:0007267 TAS
    innate immune response GO:0045087 TAS
    positive regulation of signal transduction GO:0009967 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    Ras protein signal transduction GO:0007265 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    T cell costimulation GO:0031295 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified GRAP2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GRAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD28  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 LAT 27040
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 CCNDBP1 23582
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 GAB3  
Invitro Homo sapiens
5 SHC1 6464
Affinity Capture-MS Homo sapiens
6 RBPMS  
Two-hybrid Homo sapiens
7 YWHAE 7531
Two-hybrid Homo sapiens
8 GFAP 2670
Two-hybrid Homo sapiens
9 FASLG 356
Invivo Homo sapiens
Invitro Homo sapiens
10 GAB2 9846
Invitro Homo sapiens
11 HNRNPK 3190
Two-hybrid Homo sapiens
12 GAB1  
Two-hybrid Homo sapiens
13 KRTAP4-12  
Two-hybrid Homo sapiens
14 LCP2 3937
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
15 ZBTB7B  
Two-hybrid Homo sapiens
16 SOS2 6655
Reconstituted Complex Homo sapiens
17 GNB2L1 10399
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 KHDRBS1 10657
Reconstituted Complex Homo sapiens
19 STAMBP 10617
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
20 FLJ11588  
Two-hybrid Homo sapiens
21 CSF1R  
Reconstituted Complex Homo sapiens
22 MAP4K1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Phenotypic Enhancement Homo sapiens
View the network image/svg+xml



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