Gene description for SPRY2
Gene name sprouty homolog 2 (Drosophila)
Gene symbol SPRY2
Other names/aliases hSPRY2
Species Homo sapiens
 Database cross references - SPRY2
ExoCarta ExoCarta_10253
Vesiclepedia VP_10253
Entrez Gene 10253
HGNC 11270
MIM 602466
UniProt O43597  
 SPRY2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SPRY2
Molecular Function
    protein kinase binding GO:0019901 IPI
    protein serine/threonine kinase inhibitor activity GO:0030291 IC
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activator activity GO:0043539 IMP
Biological Process
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IEA
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    negative regulation of MAP kinase activity GO:0043407 IEA
    inner ear morphogenesis GO:0042472 IEA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    sensory perception of sound GO:0007605 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IDA
    cell fate commitment GO:0045165 IEA
    regulation of cell differentiation GO:0045595 IEA
    bud elongation involved in lung branching GO:0060449 IEA
    positive regulation of protein kinase B signaling GO:0051897 IMP
    negative regulation of cell proliferation GO:0008285 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    establishment of mitotic spindle orientation GO:0000132 IEA
    positive regulation of protein serine/threonine kinase activity GO:0071902 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of neurotrophin TRK receptor signaling pathway GO:0051387 IEA
    lung growth GO:0060437 IEA
Subcellular Localization
    microtubule cytoskeleton GO:0015630 IDA
    microtubule GO:0005874 IEA
    ruffle membrane GO:0032587 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified SPRY2 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPRY2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBL 867
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 TSRC1  
Two-hybrid Homo sapiens
3 KRTAP4-12  
Two-hybrid Homo sapiens
4 TRAF2 7186
Two-hybrid Homo sapiens
5 PLSCR1 5359
Two-hybrid Homo sapiens
6 GRB2 2885
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
7 TRIM51  
Two-hybrid Homo sapiens
8 KIAA0514  
Two-hybrid Homo sapiens
9 RAF1 5894
Affinity Capture-Western Homo sapiens
10 CBLC  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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