Gene description for UBE2D2
Gene name ubiquitin-conjugating enzyme E2D 2
Gene symbol UBE2D2
Other names/aliases E2(17)KB2
PUBC1
UBC4
UBC4/5
UBCH4
UBCH5B
Species Homo sapiens
 Database cross references - UBE2D2
ExoCarta ExoCarta_7322
Entrez Gene 7322
HGNC 12475
MIM 602962
UniProt P62837  
 UBE2D2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
 Gene ontology annotations for UBE2D2
Molecular Function
    ATP binding GO:0005524 IEA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
Biological Process
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    protein ubiquitination GO:0016567 IDA
    cellular response to hypoxia GO:0071456 TAS
    protein K48-linked ubiquitination GO:0070936 IDA
    cellular protein modification process GO:0006464 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    protein polyubiquitination GO:0000209 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    protein autoubiquitination GO:0051865 IDA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    innate immune response GO:0045087 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
    nucleoplasm GO:0005654 TAS
    protein complex GO:0043234 IDA
    ubiquitin ligase complex GO:0000151 IDA
 Experiment description of studies that identified UBE2D2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for UBE2D2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBL 867
Invitro Homo sapiens
2 RNF2  
Biochemical Activity Homo sapiens
3 NEDD4 4734
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
4 PJA1  
Invitro Homo sapiens
Invivo Homo sapiens
5 UBE3A 7337
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
6 DTX3L 151636
Affinity Capture-Western Homo sapiens
7 BARD1 580
Reconstituted Complex Homo sapiens
8 PJA2  
Invivo Homo sapiens
Invitro Homo sapiens
9 AMFR 267
Invivo Homo sapiens
Invitro Homo sapiens
10 TRIM5  
Biochemical Activity Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here