Gene description for RNF149
Gene name ring finger protein 149
Gene symbol RNF149
Other names/aliases DNAPTP2
Species Homo sapiens
 Database cross references - RNF149
ExoCarta ExoCarta_284996
Entrez Gene 284996
HGNC 23137
UniProt Q8NC42  
 RNF149 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RNF149
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IEA
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    cellular response to drug GO:0035690 IEA
    protein ubiquitination GO:0016567 IEA
    negative regulation of MAPK cascade GO:0043409 IEA
    regulation of protein stability GO:0031647 IEA
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified RNF149 in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RNF149
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNF149 is involved
No pathways found





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