Gene ontology annotations for BROX |
|
Experiment description of studies that identified BROX in exosomes |
1 |
Experiment ID |
20 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP90
|
EV Cytosolic markers |
✔
CD63|CD81|LAMP1
|
EV Membrane markers |
✔
GOLGA2|cytochrome c
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17956143
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
Authors |
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS |
Journal name |
JPR
|
Publication year |
2007 |
Sample |
Colorectal cancer cells |
Sample name |
HT29 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Diafiltration |
Flotation density |
1.16 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |
|
|
2 |
Experiment ID |
21 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
19837982
|
Organism |
Homo sapiens |
Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
Authors |
Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson |
Journal name |
MCP
|
Publication year |
2009 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1215 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.10-1.12 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [Orbitrap] Western blotting |
|
|
3 |
Experiment ID |
207 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DKO-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
208 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
Dks-8 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
5 |
Experiment ID |
209 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DLD-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
6 |
Experiment ID |
282 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
7 |
Experiment ID |
283 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
8 |
Experiment ID |
285 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
9 |
Experiment ID |
286 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
10 |
Experiment ID |
234 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✔
qNano
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
26054723
|
Organism |
Homo sapiens |
Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
Journal name |
Carcinogenesis
|
Publication year |
2015 |
Sample |
Hepatocellular carcinoma cells |
Sample name |
HKCI-C3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein RNA |
Methods used in the study |
Western blotting Mass spectrometry RT-PCR RNA Sequencing |
|
|
11 |
Experiment ID |
236 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✔
qNano
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
26054723
|
Organism |
Homo sapiens |
Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
Journal name |
Carcinogenesis
|
Publication year |
2015 |
Sample |
Hepatocellular carcinoma cells |
Sample name |
MHCC97L |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein RNA |
Methods used in the study |
Western blotting Mass spectrometry RT-PCR RNA Sequencing |
|
|
12 |
Experiment ID |
254 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
MNT-1 |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
13 |
Experiment ID |
255 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
G1 |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
14 |
Experiment ID |
256 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
501mel |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
15 |
Experiment ID |
257 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
Daju |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
16 |
Experiment ID |
258 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
SKMEL28 |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
17 |
Experiment ID |
259 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
A375M |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
18 |
Experiment ID |
260 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
1205Lu |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
19 |
Experiment ID |
224 |
ISEV standards |
✔
EM|AFM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✔
GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25944692
|
Organism |
Homo sapiens |
Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
SH-SY5Y |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
20 |
Experiment ID |
211 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
cytochrome c|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
21 |
Experiment ID |
212 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
22 |
Experiment ID |
231 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
23 |
Experiment ID |
232 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
24 |
Experiment ID |
233 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25332113
|
Organism |
Homo sapiens |
Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
Journal name |
Transfusion
|
Publication year |
2015 |
Sample |
Platelets |
Sample name |
PL-Exs - Rep 3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient |
Flotation density |
1.12-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
25 |
Experiment ID |
66 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19199708
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). |
Authors |
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR |
Journal name |
JPR
|
Publication year |
2009 |
Sample |
Saliva |
Sample name |
Saliva |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy |
|
|
26 |
Experiment ID |
191 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD81|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
27 |
Experiment ID |
13 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
Alix|RAB4|RAB5B|RAB11|TSG101
|
EV Cytosolic markers |
✔
CD9|AQP2|AQP1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
15326289
|
Organism |
Homo sapiens |
Experiment description |
Identification and proteomic profiling of exosomes in human urine. |
Authors |
Pisitkun T, Shen RF, Knepper MA |
Journal name |
PNAS
|
Publication year |
2004 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LCQ DECA XP] Western blotting |
|
|
28 |
Experiment ID |
63 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
AQP2
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19056867
|
Organism |
Homo sapiens |
Experiment description |
Large-scale proteomics and phosphoproteomics of urinary exosomes. |
Authors |
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA |
Journal name |
JASN
|
Publication year |
2009 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |
|
|
29 |
Experiment ID |
193 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✔
PHB
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21595033
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
Authors |
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. |
Journal name |
Proteomics
|
Publication year |
2011 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
30 |
Experiment ID |
194 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21595033
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
Authors |
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. |
Journal name |
Proteomics
|
Publication year |
2011 |
Sample |
Urine |
Sample name |
Urine - Patients of basement membrane nephropathy |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
31 |
Experiment ID |
195 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21595033
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
Authors |
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. |
Journal name |
Proteomics
|
Publication year |
2011 |
Sample |
Urine |
Sample name |
Urine - Patients of early IgA nephropathy |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
32 |
Experiment ID |
196 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal high density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
33 |
Experiment ID |
197 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal low density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
Protein-protein interactions for BROX |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which BROX is involved |
No pathways found
|
|
|