Gene description for SPR
Gene name sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
Gene symbol SPR
Other names/aliases SDR38C1
Species Homo sapiens
 Database cross references - SPR
ExoCarta ExoCarta_6697
Entrez Gene 6697
HGNC 11257
MIM 182125
UniProt P35270  
 SPR identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for SPR
Molecular Function
    aldo-keto reductase (NADP) activity GO:0004033 TAS
    sepiapterin reductase activity GO:0004757 TAS
    NADP binding GO:0050661 TAS
Biological Process
    dopamine metabolic process GO:0042417 IEA
    death GO:0016265 IEA
    oxidation-reduction process GO:0055114 TAS
    norepinephrine metabolic process GO:0042415 IEA
    nitric oxide biosynthetic process GO:0006809 IDA
    voluntary musculoskeletal movement GO:0050882 IEA
    regulation of nitric-oxide synthase activity GO:0050999 TAS
    tetrahydrobiopterin biosynthetic process GO:0006729 TAS
    regulation of multicellular organism growth GO:0040014 IEA
    small molecule metabolic process GO:0044281 TAS
    pteridine metabolic process GO:0019889 IEA
    L-phenylalanine metabolic process GO:0006558 IEA
    nitric oxide metabolic process GO:0046209 TAS
    cell morphogenesis involved in neuron differentiation GO:0048667 IEA
    serotonin metabolic process GO:0042428 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SPR in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SPR
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SPR is involved
PathwayEvidenceSource
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation TAS Reactome





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