Gene description for ASS1
Gene name argininosuccinate synthase 1
Gene symbol ASS1
Other names/aliases ASS
CTLN1
Species Homo sapiens
 Database cross references - ASS1
ExoCarta ExoCarta_445
Entrez Gene 445
HGNC 758
MIM 603470
UniProt P00966  
 ASS1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ASS1
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    identical protein binding GO:0042802 IPI
    toxic substance binding GO:0015643 IEA
    ATP binding GO:0005524 IEA
    amino acid binding GO:0016597 IMP
    argininosuccinate synthase activity GO:0004055 IMP
Biological Process
    midgut development GO:0007494 IEA
    liver development GO:0001889 IEA
    aging GO:0007568 IEA
    response to zinc ion GO:0010043 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    diaphragm development GO:0060539 IEA
    kidney development GO:0001822 IEA
    response to nutrient GO:0007584 IEA
    cellular response to interferon-gamma GO:0071346 IEA
    acute-phase response GO:0006953 IEA
    response to estradiol GO:0032355 IEA
    citrulline metabolic process GO:0000052 IMP
    cellular response to cAMP GO:0071320 IEA
    small molecule metabolic process GO:0044281 TAS
    response to drug GO:0042493 IEA
    cellular response to amine stimulus GO:0071418 IEA
    negative regulation of leukocyte cell-cell adhesion GO:1903038 IMP
    cellular response to laminar fluid shear stress GO:0071499 IMP
    response to growth hormone GO:0060416 IEA
    arginine biosynthetic process GO:0006526 IEA
    cellular response to amino acid stimulus GO:0071230 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    aspartate metabolic process GO:0006531 IMP
    cellular nitrogen compound metabolic process GO:0034641 TAS
    cellular response to ammonium ion GO:0071242 IEA
    argininosuccinate metabolic process GO:0000053 IMP
    cellular response to oleic acid GO:0071400 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    urea cycle GO:0000050 TAS
    response to mycotoxin GO:0010046 IEA
    cellular response to glucagon stimulus GO:0071377 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    perikaryon GO:0043204 IEA
    lysosome GO:0005764 IEA
    cell body fiber GO:0070852 IEA
    nucleus GO:0005634 IEA
    endoplasmic reticulum GO:0005783 IEA
    mitochondrial outer membrane GO:0005741 IEA
 Experiment description of studies that identified ASS1 in exosomes
1
Experiment ID 76
ISEV standards
EM
EV Biophysical techniques
TSG101|GAPDH|HSP90
EV Cytosolic markers
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
7
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASCC2  
Two-hybrid Homo sapiens
2 ARAF 369
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 FTS  
Two-hybrid Homo sapiens
4 ASS1 445
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ASS1 is involved
PathwayEvidenceSource
Urea cycle TAS Reactome





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