Gene description for APOO
Gene name apolipoprotein O
Gene symbol APOO
Other names/aliases FAM121B
My025
Species Homo sapiens
 Database cross references - APOO
ExoCarta ExoCarta_79135
Vesiclepedia VP_79135
Entrez Gene 79135
HGNC 28727
MIM 300753
UniProt Q9BUR5  
 APOO identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for APOO
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    lipid transport GO:0006869 IEA
    inner mitochondrial membrane organization GO:0007007 IC
    cristae formation GO:0042407 IBA
    cristae formation GO:0042407 IMP
    cristae formation GO:0042407 NAS
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    SAM complex GO:0001401 HDA
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 ISS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 NAS
    endoplasmic reticulum membrane GO:0005789 IDA
    cytosol GO:0005829 IDA
    very-low-density lipoprotein particle GO:0034361 IEA
    low-density lipoprotein particle GO:0034362 IEA
    high-density lipoprotein particle GO:0034364 IEA
    mitochondrial crista junction GO:0044284 NAS
    MICOS complex GO:0061617 HDA
    MICOS complex GO:0061617 IBA
    MICOS complex GO:0061617 IDA
    MICOS complex GO:0061617 NAS
    MIB complex GO:0140275 HDA
 Experiment description of studies that identified APOO in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for APOO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTR2 10097
Proximity Label-MS Homo sapiens
2 AFG3L2 10939
Proximity Label-MS Homo sapiens
3 UQCRFS1 7386
Proximity Label-MS Homo sapiens
4 ARHGEF2 9181
Proximity Label-MS Homo sapiens
5 DNAJC11 55735
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
6 NDUFS1 4719
Proximity Label-MS Homo sapiens
7 SAMM50 25813
Proximity Label-MS Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 SLC25A13 10165
Proximity Label-MS Homo sapiens
10 NSDHL 50814
Proximity Label-MS Homo sapiens
11 ATN1  
Proximity Label-MS Homo sapiens
12 HSD17B12 51144
Proximity Label-MS Homo sapiens
13 NDUFS2 4720
Proximity Label-MS Homo sapiens
14 HCCS 3052
Proximity Label-MS Homo sapiens
15 CHCHD4  
Proximity Label-MS Homo sapiens
16 NDUFB9 4715
Proximity Label-MS Homo sapiens
17 MARK2 2011
Proximity Label-MS Homo sapiens
18 NDUFS3 4722
Proximity Label-MS Homo sapiens
19 TIMM8A 1678
Proximity Label-MS Homo sapiens
20 NDUFB8 4714
Proximity Label-MS Homo sapiens
21 TIMMDC1  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
22 TIMM17B  
Proximity Label-MS Homo sapiens
23 YARS2  
Proximity Label-MS Homo sapiens
24 NDUFA5 4698
Proximity Label-MS Homo sapiens
25 SFXN4  
Proximity Label-MS Homo sapiens
26 C12orf73  
Proximity Label-MS Homo sapiens
27 LACTB 114294
Proximity Label-MS Homo sapiens
28 NDUFAF1  
Proximity Label-MS Homo sapiens
29 PHB 5245
Proximity Label-MS Homo sapiens
30 SLC25A25  
Proximity Label-MS Homo sapiens
31 SLC30A9 10463
Proximity Label-MS Homo sapiens
32 UQCC3  
Proximity Label-MS Homo sapiens
33 MPC2 25874
Proximity Label-MS Homo sapiens
34 CPOX 1371
Proximity Label-MS Homo sapiens
35 SFXN1 94081
Proximity Label-MS Homo sapiens
36 PARPBP  
Affinity Capture-MS Homo sapiens
37 STOML2 30968
Proximity Label-MS Homo sapiens
38 E2F4  
Affinity Capture-MS Homo sapiens
39 UBR4 23352
Proximity Label-MS Homo sapiens
40 OCIAD2 132299
Proximity Label-MS Homo sapiens
41 ATAD3B 83858
Proximity Label-MS Homo sapiens
42 SPG21 51324
Affinity Capture-MS Homo sapiens
43 NUBP2 10101
Proximity Label-MS Homo sapiens
44 COX15 1355
Proximity Label-MS Homo sapiens
45 TMEM126A 84233
Proximity Label-MS Homo sapiens
46 EXOG 9941
Proximity Label-MS Homo sapiens
47 CHCHD3 54927
Proximity Label-MS Homo sapiens
48 HSP90B1 7184
Proximity Label-MS Homo sapiens
49 NDUFA8 4702
Proximity Label-MS Homo sapiens
50 MICU2 221154
Proximity Label-MS Homo sapiens
51 SNAP47 116841
Proximity Label-MS Homo sapiens
52 MTX2 10651
Proximity Label-MS Homo sapiens
53 PTCD3 55037
Proximity Label-MS Homo sapiens
54 CENPM  
Affinity Capture-MS Homo sapiens
55 OPA1 4976
Proximity Label-MS Homo sapiens
56 TMEM126B 55863
Proximity Label-MS Homo sapiens
57 CYC1 1537
Proximity Label-MS Homo sapiens
58 COA7  
Proximity Label-MS Homo sapiens
59 CHCHD6 84303
Proximity Label-MS Homo sapiens
60 MICU1 10367
Proximity Label-MS Homo sapiens
61 NDUFAF3 25915
Proximity Label-MS Homo sapiens
62 CKMT1B 1159
Proximity Label-MS Homo sapiens
63 COX4I1 1327
Proximity Label-MS Homo sapiens
64 ENDOG  
Proximity Label-MS Homo sapiens
65 GSX1  
Affinity Capture-MS Homo sapiens
66 OXA1L  
Proximity Label-MS Homo sapiens
67 BIRC2  
Proximity Label-MS Homo sapiens
68 SLC25A24 29957
Proximity Label-MS Homo sapiens
69 COX5B 1329
Proximity Label-MS Homo sapiens
70 SLC25A12 8604
Proximity Label-MS Homo sapiens
71 FANCD2  
Proximity Label-MS Homo sapiens
72 AIFM1 9131
Proximity Label-MS Homo sapiens
73 NDUFB10 4716
Proximity Label-MS Homo sapiens
74 NDUFA13 51079
Proximity Label-MS Homo sapiens
75 NDUFA9 4704
Proximity Label-MS Homo sapiens
76 LETM1 3954
Proximity Label-MS Homo sapiens
77 ASS1 445
Proximity Label-MS Homo sapiens
78 GPD2 2820
Proximity Label-MS Homo sapiens
79 COX6B1 1340
Proximity Label-MS Homo sapiens
80 TMEM242  
Proximity Label-MS Homo sapiens
81 OCIAD1 54940
Proximity Label-MS Homo sapiens
82 TM9SF3 56889
Proximity Label-MS Homo sapiens
83 HIGD2A  
Proximity Label-MS Homo sapiens
84 CDK2 1017
Affinity Capture-MS Homo sapiens
85 YME1L1 10730
Proximity Label-MS Homo sapiens
86 RPA3 6119
Proximity Label-MS Homo sapiens
87 APOOL 139322
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
88 COX6C 1345
Proximity Label-MS Homo sapiens
89 OMA1  
Proximity Label-MS Homo sapiens
90 IDI1 3422
Proximity Label-MS Homo sapiens
91 C19orf70  
Affinity Capture-MS Homo sapiens
92 HSDL2 84263
Proximity Label-MS Homo sapiens
93 NCLN 56926
Proximity Label-MS Homo sapiens
94 SCO1  
Proximity Label-MS Homo sapiens
95 ACAD9 28976
Proximity Label-MS Homo sapiens
96 HYOU1 10525
Proximity Label-MS Homo sapiens
97 ABCB10 23456
Proximity Label-MS Homo sapiens
98 CLPB 81570
Proximity Label-MS Homo sapiens
99 PARL  
Proximity Label-MS Homo sapiens
100 HAX1  
Proximity Label-MS Homo sapiens
101 COX2 4513
Proximity Label-MS Homo sapiens
102 LRRC40 55631
Proximity Label-MS Homo sapiens
103 BMP2K 55589
Proximity Label-MS Homo sapiens
104 CYCS 54205
Proximity Label-MS Homo sapiens
105 FLOT1 10211
Proximity Label-MS Homo sapiens
106 MTHFD1L 25902
Proximity Label-MS Homo sapiens
107 IMMT 10989
Proximity Label-MS Homo sapiens
108 SUN1 23353
Proximity Label-MS Homo sapiens
109 PHB2 11331
Proximity Label-MS Homo sapiens
110 TMED10 10972
Proximity Label-MS Homo sapiens
111 TRAF2 7186
Proximity Label-MS Homo sapiens
112 PDIA4 9601
Proximity Label-MS Homo sapiens
113 MRPS31  
Proximity Label-MS Homo sapiens
114 COX5A 9377
Proximity Label-MS Homo sapiens
115 ADCK2  
Proximity Label-MS Homo sapiens
116 NDUFS7 374291
Proximity Label-MS Homo sapiens
117 PTBP3 9991
Proximity Label-MS Homo sapiens
118 TOMM70A 9868
Proximity Label-MS Homo sapiens
119 UBE3C 9690
Proximity Label-MS Homo sapiens
120 ATAD3A 55210
Proximity Label-MS Homo sapiens
121 UFL1 23376
Proximity Label-MS Homo sapiens
122 SMIM12  
Proximity Label-MS Homo sapiens
123 NDUFS8 4728
Proximity Label-MS Homo sapiens
124 MMS19 64210
Proximity Label-MS Homo sapiens
125 SLC25A4 291
Proximity Label-MS Homo sapiens
126 C19orf52  
Proximity Label-MS Homo sapiens
127 COX7A2 1347
Proximity Label-MS Homo sapiens
128 NDUFAF4 29078
Proximity Label-MS Homo sapiens
129 CEP85  
Proximity Label-MS Homo sapiens
130 MTX3  
Proximity Label-MS Homo sapiens
131 TIMM13 26517
Proximity Label-MS Homo sapiens
132 TIMM44 10469
Proximity Label-MS Homo sapiens
133 SNAP29 9342
Proximity Label-MS Homo sapiens
134 ECSIT 51295
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which APOO is involved
PathwayEvidenceSource
Cristae formation IEA Reactome
Mitochondrial biogenesis IEA Reactome
Organelle biogenesis and maintenance IEA Reactome





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