Gene description for NDUFAF3
Gene name NADH dehydrogenase (ubiquinone) complex I, assembly factor 3
Gene symbol NDUFAF3
Other names/aliases 2P1
C3orf60
E3-3
Species Homo sapiens
 Database cross references - NDUFAF3
ExoCarta ExoCarta_25915
Vesiclepedia VP_25915
Entrez Gene 25915
HGNC 29918
MIM 612911
UniProt Q9BU61  
 NDUFAF3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFAF3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitochondrial respiratory chain complex I assembly GO:0032981 IBA
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified NDUFAF3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFAF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLIRP 81892
Proximity Label-MS Homo sapiens
2 CABP2  
Two-hybrid Homo sapiens
3 TRIAP1  
Co-fractionation Homo sapiens
4 DUSP8  
Affinity Capture-MS Homo sapiens
5 REL 5966
Two-hybrid Homo sapiens
6 TRUB2  
Proximity Label-MS Homo sapiens
7 MTG2  
Proximity Label-MS Homo sapiens
8 NGRN  
Proximity Label-MS Homo sapiens
9 CHCHD1  
Proximity Label-MS Homo sapiens
10 EYA2  
Affinity Capture-MS Homo sapiens
11 NDUFA4 4697
Affinity Capture-MS Homo sapiens
12 DNAJC15  
Proximity Label-MS Homo sapiens
13 NDUFS2 4720
Affinity Capture-MS Homo sapiens
14 CCDC90B  
Proximity Label-MS Homo sapiens
15 LRPPRC 10128
Proximity Label-MS Homo sapiens
16 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PMPCA 23203
Proximity Label-MS Homo sapiens
18 COQ9  
Affinity Capture-MS Homo sapiens
19 C8orf82  
Proximity Label-MS Homo sapiens
20 TIMMDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HINT2 84681
Proximity Label-MS Homo sapiens
22 MRPS26 64949
Proximity Label-MS Homo sapiens
23 SURF1  
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
24 EXD2  
Proximity Label-MS Homo sapiens
25 NDUFAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PMPCB 9512
Proximity Label-MS Homo sapiens
27 TACO1  
Proximity Label-MS Homo sapiens
28 VWA8 23078
Proximity Label-MS Homo sapiens
29 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ND6  
Affinity Capture-MS Homo sapiens
31 MDH2 4191
Proximity Label-MS Homo sapiens
32 GOLGA6L9  
Two-hybrid Homo sapiens
33 APOO 79135
Proximity Label-MS Homo sapiens
34 RNF146  
Two-hybrid Homo sapiens
35 C6orf203  
Proximity Label-MS Homo sapiens
36 NDUFS4 4724
Co-fractionation Homo sapiens
37 COX15 1355
Proximity Label-MS Homo sapiens
38 FASTKD5  
Proximity Label-MS Homo sapiens
39 UHMK1  
Two-hybrid Homo sapiens
40 NDUFA8 4702
Affinity Capture-MS Homo sapiens
41 GFM1 85476
Proximity Label-MS Homo sapiens
42 TUFM 7284
Proximity Label-MS Homo sapiens
43 AGTRAP 57085
Two-hybrid Homo sapiens
44 HSCB 150274
Proximity Label-MS Homo sapiens
45 NDUFAF5  
Affinity Capture-MS Homo sapiens
46 GEM  
Two-hybrid Homo sapiens
47 CYSRT1 375791
Two-hybrid Homo sapiens
48 CCDC109B 55013
Proximity Label-MS Homo sapiens
49 TMEM126B 55863
Affinity Capture-MS Homo sapiens
50 CYC1 1537
Co-fractionation Homo sapiens
51 MRPL11 65003
Proximity Label-MS Homo sapiens
52 CEP76  
Affinity Capture-MS Homo sapiens
53 DNAJC30  
Proximity Label-MS Homo sapiens
54 TMEM70  
Proximity Label-MS Homo sapiens
55 COX4I1 1327
Proximity Label-MS Homo sapiens
56 NDUFB5 4711
Affinity Capture-MS Homo sapiens
57 IFT122 55764
Affinity Capture-MS Homo sapiens
58 C15orf48  
Affinity Capture-MS Homo sapiens
59 MRRF  
Proximity Label-MS Homo sapiens
60 MRPS12  
Proximity Label-MS Homo sapiens
61 NDUFB10 4716
Affinity Capture-MS Homo sapiens
62 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 MEX3A  
Affinity Capture-RNA Homo sapiens
64 RMND1 55005
Proximity Label-MS Homo sapiens
65 KRTAP10-3  
Two-hybrid Homo sapiens
66 KRT27 342574
Two-hybrid Homo sapiens
67 CDC42 998
Co-fractionation Homo sapiens
68 MYC  
Affinity Capture-MS Homo sapiens
69 ABCC1 4363
Co-fractionation Homo sapiens
70 ACAD9 28976
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 DUSP9  
Affinity Capture-MS Homo sapiens
72 C9orf72  
Affinity Capture-MS Homo sapiens
73 COA4  
Co-fractionation Homo sapiens
74 CLPP 8192
Proximity Label-MS Homo sapiens
75 DNAJC28  
Proximity Label-MS Homo sapiens
76 CS 1431
Proximity Label-MS Homo sapiens
77 C17orf80 55028
Proximity Label-MS Homo sapiens
78 TSFM 10102
Proximity Label-MS Homo sapiens
79 AUH 549
Proximity Label-MS Homo sapiens
80 DNAJC19 131118
Proximity Label-MS Homo sapiens
81 FOXRED2  
Affinity Capture-MS Homo sapiens
82 ICT1 3396
Affinity Capture-MS Homo sapiens
83 LONP1 9361
Proximity Label-MS Homo sapiens
84 POU2AF1  
Two-hybrid Homo sapiens
85 INCA1  
Two-hybrid Homo sapiens
86 BOP1 23246
Affinity Capture-MS Homo sapiens
87 SNRPA 6626
Two-hybrid Homo sapiens
88 DNAJA3 9093
Proximity Label-MS Homo sapiens
89 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 TKT 7086
Co-fractionation Homo sapiens
91 C21orf33  
Proximity Label-MS Homo sapiens
92 MRPS21  
Co-fractionation Homo sapiens
93 NDUFS8 4728
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
94 TBRG4 9238
Proximity Label-MS Homo sapiens
95 DHRS4 10901
Affinity Capture-MS Homo sapiens
96 MCU 90550
Proximity Label-MS Homo sapiens
97 C1QBP 708
Proximity Label-MS Homo sapiens
98 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
99 NDUFB11 54539
Co-fractionation Homo sapiens
100 MBD3L1  
Two-hybrid Homo sapiens
101 METTL17  
Proximity Label-MS Homo sapiens
102 RBCK1  
Two-hybrid Homo sapiens
103 ECSIT 51295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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