Gene description for NDUFB5
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
Gene symbol NDUFB5
Other names/aliases CISGDH
SGDH
Species Homo sapiens
 Database cross references - NDUFB5
ExoCarta ExoCarta_4711
Vesiclepedia VP_4711
Entrez Gene 4711
HGNC 7700
MIM 603841
UniProt O43674  
 NDUFB5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFB5
Molecular Function
    protein binding GO:0005515 IPI
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFB5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFB5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ruvbl1 56505
Affinity Capture-MS Mus musculus
2 NDUFS1 4719
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
3 MTX1 4580
Co-fractionation Homo sapiens
4 NCALD 83988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RPA2 6118
Proximity Label-MS Homo sapiens
6 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SAMD1  
Affinity Capture-MS Homo sapiens
8 NDUFA4 4697
Affinity Capture-MS Homo sapiens
9 SPAG9 9043
Affinity Capture-MS Homo sapiens
10 FFAR1  
Affinity Capture-MS Homo sapiens
11 NDUFS2 4720
Affinity Capture-MS Homo sapiens
12 NDUFS7 374291
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NDUFB9 4715
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PSMG3 84262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NDUFB8 4714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPR34  
Affinity Capture-MS Homo sapiens
19 NDUFA7 4701
Affinity Capture-MS Homo sapiens
20 TIMMDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FAM174A 345757
Affinity Capture-MS Homo sapiens
22 NDUFA5 4698
Affinity Capture-MS Homo sapiens
23 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MRPS26 64949
Co-fractionation Homo sapiens
25 Ncstn 59287
Affinity Capture-MS Mus musculus
26 LAMTOR3 8649
Co-fractionation Homo sapiens
27 TMEM140  
Affinity Capture-MS Homo sapiens
28 MARCKSL1 65108
Co-fractionation Homo sapiens
29 NDUFAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NDUFV3 4731
Affinity Capture-MS Homo sapiens
31 NDUFA11 126328
Affinity Capture-MS Homo sapiens
32 NCSTN 23385
Affinity Capture-MS Homo sapiens
33 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SLC25A3 5250
Co-fractionation Homo sapiens
35 NDUFB1 4707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 EXTL3 2137
Affinity Capture-MS Homo sapiens
38 DLD 1738
Co-fractionation Homo sapiens
39 MPV17 4358
Co-fractionation Homo sapiens
40 NDUFS4 4724
Co-fractionation Homo sapiens
41 NDUFB7 4713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ND2 4536
Affinity Capture-MS Homo sapiens
43 ITM2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 FAM192A  
Affinity Capture-MS Homo sapiens
46 SCAND1  
Two-hybrid Homo sapiens
47 HDAC10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KDSR 2531
Affinity Capture-MS Homo sapiens
49 GALNT6 11226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ND1  
Affinity Capture-MS Homo sapiens
51 CENPM  
Affinity Capture-MS Homo sapiens
52 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
53 IPPK  
Affinity Capture-MS Homo sapiens
54 COX7B 1349
Co-fractionation Homo sapiens
55 DNAJC30  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 NDUFAF3 25915
Affinity Capture-MS Homo sapiens
57 PIPSL 266971
Affinity Capture-MS Homo sapiens
58 TMEM185A  
Affinity Capture-MS Homo sapiens
59 GJA8  
Affinity Capture-MS Homo sapiens
60 TTYH1  
Affinity Capture-MS Homo sapiens
61 AIFM1 9131
Proximity Label-MS Homo sapiens
62 NDUFB10 4716
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
63 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ND5 4540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 UQCRC2 7385
Co-fractionation Homo sapiens
67 CRELD1 78987
Affinity Capture-MS Homo sapiens
68 BCAP31 10134
Co-fractionation Homo sapiens
69 ATP5F1 515
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
70 CLEC4A  
Affinity Capture-MS Homo sapiens
71 AQP3  
Affinity Capture-MS Homo sapiens
72 OPRL1 4987
Affinity Capture-MS Homo sapiens
73 LPAR1 1902
Affinity Capture-MS Homo sapiens
74 NDUFB3 4709
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 RPA3 6119
Proximity Label-MS Homo sapiens
76 COX6C 1345
Co-fractionation Homo sapiens
77 CYP2J2  
Affinity Capture-MS Homo sapiens
78 ATP6V1A 523
Co-fractionation Homo sapiens
79 MSLN 10232
Co-fractionation Homo sapiens
80 NDUFB6 4712
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ACAD9 28976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 NDUFA3  
Affinity Capture-MS Homo sapiens
83 NDUFV2 4729
Affinity Capture-MS Homo sapiens
84 NDUFC2 4718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
85 ADGRE5 976
Affinity Capture-MS Homo sapiens
86 FUT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 FTL 2512
Affinity Capture-MS Homo sapiens
88 NDUFA1 4694
Affinity Capture-MS Homo sapiens
89 VNN2  
Affinity Capture-MS Homo sapiens
90 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 EIF3I 8668
Co-fractionation Homo sapiens
93 ICT1 3396
Co-fractionation Homo sapiens
94 B4GAT1 11041
Affinity Capture-MS Homo sapiens
95 PSME3 10197
Affinity Capture-MS Homo sapiens
96 SFXN1 94081
Proximity Label-MS Homo sapiens
97 NDUFS8 4728
Affinity Capture-MS Homo sapiens
98 TMEM126B 55863
Affinity Capture-MS Homo sapiens
99 SORT1 6272
Affinity Capture-MS Homo sapiens
100 SLC25A51  
Proximity Label-MS Homo sapiens
101 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 COX7C 1350
Co-fractionation Homo sapiens
103 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 ND4 4538
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 ECSIT 51295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 MTCH2 23788
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
View the network image/svg+xml



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