Gene description for NDUFA1
Gene name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
Gene symbol NDUFA1
Other names/aliases CI-MWFE
MWFE
ZNF183
Species Homo sapiens
 Database cross references - NDUFA1
ExoCarta ExoCarta_4694
Vesiclepedia VP_4694
Entrez Gene 4694
HGNC 7683
MIM 300078
UniProt O15239  
 NDUFA1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NDUFA1
Molecular Function
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial membrane GO:0031966 IDA
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFA1 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NDUFA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Affinity Capture-MS Homo sapiens
2 NDUFV3 4731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 NDUFA6  
Affinity Capture-MS Homo sapiens
5 NDUFS1 4719
Affinity Capture-MS Homo sapiens
6 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
7 NDUFS5 4725
Affinity Capture-MS Homo sapiens
8 TOR1AIP2 163590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NDUFB10 4716
Affinity Capture-MS Homo sapiens
10 NDUFA13 51079
Affinity Capture-MS Homo sapiens
11 NDUFA9 4704
Affinity Capture-MS Homo sapiens
12 NDUFB1 4707
Affinity Capture-MS Homo sapiens
13 ND5 4540
Affinity Capture-MS Homo sapiens
14 NDUFB5 4711
Affinity Capture-MS Homo sapiens
15 NDUFS4 4724
Affinity Capture-MS Homo sapiens
16 NDUFB7 4713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TRIM55  
Two-hybrid Homo sapiens
18 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NDUFB9 4715
Affinity Capture-MS Homo sapiens
20 NDUFS8 4728
Affinity Capture-MS Homo sapiens
21 NDUFB2 4708
Affinity Capture-MS Homo sapiens
22 NDUFS3 4722
Affinity Capture-MS Homo sapiens
23 NDUFB3 4709
Affinity Capture-MS Homo sapiens
24 ND1  
Affinity Capture-MS Homo sapiens
25 NDUFB8 4714
Affinity Capture-MS Homo sapiens
26 NDUFA7 4701
Affinity Capture-MS Homo sapiens
27 NDUFB6 4712
Affinity Capture-MS Homo sapiens
28 NDUFA2 4695
Affinity Capture-MS Homo sapiens
29 NDUFA5 4698
Affinity Capture-MS Homo sapiens
30 NDUFB11 54539
Affinity Capture-MS Homo sapiens
31 NDUFA10 4705
Affinity Capture-MS Homo sapiens
32 NDUFA12 55967
Affinity Capture-MS Homo sapiens
33 DNAJC30  
Affinity Capture-MS Homo sapiens
34 NDUFV2 4729
Affinity Capture-MS Homo sapiens
35 NDUFC2 4718
Affinity Capture-MS Homo sapiens
36 ND4 4538
Affinity Capture-MS Homo sapiens
37 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NDUFA3  
Affinity Capture-MS Homo sapiens
39 TRIM63  
Two-hybrid Homo sapiens
40 NDUFS2 4720
Affinity Capture-MS Homo sapiens
41 NDUFS7 374291
Affinity Capture-MS Homo sapiens
42 NDUFB4 4710
Affinity Capture-MS Homo sapiens
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