Gene description for ND5
Gene name mitochondrially encoded NADH dehydrogenase 5
Gene symbol ND5
Other names/aliases MTND5
Species Homo sapiens
 Database cross references - ND5
ExoCarta ExoCarta_4540
Vesiclepedia VP_4540
Entrez Gene 4540
HGNC 7461
MIM 516005
UniProt P03915  
 ND5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ND5
Molecular Function
    NADH dehydrogenase (ubiquinone) activity GO:0008137 IMP
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    response to hypoxia GO:0001666 IEA
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IMP
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    electron transport coupled proton transport GO:0015990 IBA
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    response to hydrogen peroxide GO:0042542 IEA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified ND5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ND5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Affinity Capture-MS Homo sapiens
2 CRELD1 78987
Affinity Capture-MS Homo sapiens
3 LIG4 3981
Two-hybrid Homo sapiens
4 FNDC4 64838
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NDUFB8 4714
Affinity Capture-MS Homo sapiens
6 NDUFA11 126328
Affinity Capture-MS Homo sapiens
7 DVL2 1856
Affinity Capture-MS Homo sapiens
8 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
10 NDUFV3 4731
Affinity Capture-MS Homo sapiens
11 VAPB 9217
Affinity Capture-MS Homo sapiens
12 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NDUFA1 4694
Affinity Capture-MS Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
15 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DNAJC30  
Affinity Capture-MS Homo sapiens
17 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 EXTL3 2137
Affinity Capture-MS Homo sapiens
19 NDUFB7 4713
Affinity Capture-MS Homo sapiens
20 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 OPALIN  
Affinity Capture-MS Homo sapiens
22 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MGARP  
Affinity Capture-MS Homo sapiens
24 NDUFS4 4724
Affinity Capture-MS Homo sapiens
25 TSPAN15 23555
Affinity Capture-MS Homo sapiens
26 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MRPL50 54534
Affinity Capture-MS Homo sapiens
28 KLRB1  
Affinity Capture-MS Homo sapiens
29 TMEM72  
Affinity Capture-MS Homo sapiens
30 AQP3  
Affinity Capture-MS Homo sapiens
31 FFAR1  
Affinity Capture-MS Homo sapiens
32 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 GLUL 2752
Two-hybrid Homo sapiens
35 CYP2J2  
Affinity Capture-MS Homo sapiens
36 GPR34  
Affinity Capture-MS Homo sapiens
37 NDUFA7 4701
Affinity Capture-MS Homo sapiens
38 ASIC4  
Affinity Capture-MS Homo sapiens
39 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 NDUFB3 4709
Affinity Capture-MS Homo sapiens
42 NTSR1  
Affinity Capture-MS Homo sapiens
43 SLC22A4 6583
Affinity Capture-MS Homo sapiens
44 NDUFV2 4729
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 NDUFC2 4718
Affinity Capture-MS Homo sapiens
46 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MRPL18 29074
Affinity Capture-MS Homo sapiens
49 FUT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MRPL2 51069
Affinity Capture-MS Homo sapiens
51 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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