Gene description for LIG4
Gene name ligase IV, DNA, ATP-dependent
Gene symbol LIG4
Other names/aliases LIG4S
Species Homo sapiens
 Database cross references - LIG4
ExoCarta ExoCarta_3981
Vesiclepedia VP_3981
Entrez Gene 3981
HGNC 6601
MIM 601837
UniProt P49917  
 LIG4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LIG4
Molecular Function
    DNA binding GO:0003677 IDA
    DNA ligase activity GO:0003909 TAS
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IDA
    metal ion binding GO:0046872 IEA
    DNA ligase (ATP) activity GO:0003910 IDA
    ATP binding GO:0005524 IEA
    protein C-terminus binding GO:0008022 IPI
Biological Process
    cell proliferation GO:0008283 ISS
    neuron apoptotic process GO:0051402 ISS
    somatic stem cell maintenance GO:0035019 ISS
    double-strand break repair via classical nonhomologous end joining GO:0097680 IMP
    T cell differentiation in thymus GO:0033077 ISS
    cellular response to lithium ion GO:0071285 IEA
    positive regulation of fibroblast proliferation GO:0048146 ISS
    DNA ligation involved in DNA recombination GO:0051102 ISS
    double-strand break repair GO:0006302 TAS
    chromosome organization GO:0051276 ISS
    establishment of integrated proviral latency GO:0075713 TAS
    lagging strand elongation GO:0006273 IBA
    response to gamma radiation GO:0010332 ISS
    T cell receptor V(D)J recombination GO:0033153 ISS
    V(D)J recombination GO:0033151 IDA
    DNA biosynthetic process GO:0071897 IEA
    immunoglobulin V(D)J recombination GO:0033152 IBA
    cell cycle GO:0007049 IEA
    pro-B cell differentiation GO:0002328 ISS
    viral process GO:0016032 TAS
    in utero embryonic development GO:0001701 ISS
    DNA repair GO:0006281 TAS
    cell division GO:0051301 IEA
    single strand break repair GO:0000012 IDA
    response to X-ray GO:0010165 IMP
    central nervous system development GO:0007417 ISS
    DNA ligation GO:0006266 IDA
    negative regulation of neuron apoptotic process GO:0043524 ISS
    nucleotide-excision repair, DNA gap filling GO:0006297 IDA
    isotype switching GO:0045190 ISS
    double-strand break repair via nonhomologous end joining GO:0006303 TAS
    positive regulation of neurogenesis GO:0050769 ISS
    DNA ligation involved in DNA repair GO:0051103 ISS
Subcellular Localization
    DNA ligase IV complex GO:0032807 IMP
    focal adhesion GO:0005925 IDA
    DNA-dependent protein kinase-DNA ligase 4 complex GO:0005958 ISS
    nucleus GO:0005634 IDA
    nonhomologous end joining complex GO:0070419 IDA
    plasma membrane GO:0005886 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    condensed chromosome GO:0000793 IDA
 Experiment description of studies that identified LIG4 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LIG4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XRCC4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
2 ATR  
Protein-peptide Homo sapiens
3 FLJ12610  
Affinity Capture-Western Homo sapiens
4 ATM 472
Protein-peptide Homo sapiens
5 PNKP 11284
Affinity Capture-MS Homo sapiens
6 PRKDC 5591
Protein-peptide Homo sapiens
View the network image/svg+xml
 Pathways in which LIG4 is involved
PathwayEvidenceSource
2-LTR circle formation TAS Reactome
Nonhomologous End-joining (NHEJ) TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here