Gene description for THOC5
Gene name THO complex 5
Gene symbol THOC5
Other names/aliases C22orf19
Fmip
PK1.3
fSAP79
Species Homo sapiens
 Database cross references - THOC5
ExoCarta ExoCarta_8563
Vesiclepedia VP_8563
Entrez Gene 8563
HGNC 19074
MIM 612733
UniProt Q13769  
 THOC5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
 Gene ontology annotations for THOC5
Molecular Function
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
Biological Process
    mRNA processing GO:0006397 IEA
    mRNA export from nucleus GO:0006406 IBA
    mRNA export from nucleus GO:0006406 IDA
    mRNA export from nucleus GO:0006406 IMP
    RNA splicing GO:0008380 IEA
    monocyte differentiation GO:0030224 IDA
    primitive hemopoiesis GO:0060215 ISS
Subcellular Localization
    transcription export complex GO:0000346 IDA
    THO complex GO:0000347 IDA
    THO complex part of transcription export complex GO:0000445 IBA
    THO complex part of transcription export complex GO:0000445 IDA
    chromosome, telomeric region GO:0000781 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified THOC5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for THOC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 LIG4 3981
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 GTF2F1 2962
Affinity Capture-MS Homo sapiens
4 LUZP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 UTRN 7402
Co-fractionation Homo sapiens
6 NCBP1 4686
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 PPIE 10450
Affinity Capture-MS Homo sapiens
8 ZNF606  
Co-fractionation Homo sapiens
9 RIT1 6016
Negative Genetic Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 ZC3H14  
Affinity Capture-MS Homo sapiens
12 THOC2 57187
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
13 C9orf78 51759
Affinity Capture-MS Homo sapiens
14 THOC7 80145
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
15 CCDC9  
Affinity Capture-MS Homo sapiens
16 TNFAIP6 7130
Affinity Capture-MS Homo sapiens
17 MRPS27 23107
Affinity Capture-MS Homo sapiens
18 HSF2  
Affinity Capture-MS Homo sapiens
19 RNH1 6050
Co-fractionation Homo sapiens
20 FMNL1 752
Affinity Capture-MS Homo sapiens
21 DDX23 9416
Co-fractionation Homo sapiens
22 POLDIP3 84271
Affinity Capture-MS Homo sapiens
23 DDX24 57062
Affinity Capture-MS Homo sapiens
24 WDR74 54663
Affinity Capture-MS Homo sapiens
25 THOC6 79228
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
26 PRC1 9055
Affinity Capture-MS Homo sapiens
27 MAGOHB 55110
Affinity Capture-MS Homo sapiens
28 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SNRPC 6631
Affinity Capture-MS Homo sapiens
30 Tagap  
Affinity Capture-MS Mus musculus
31 SARNP 84324
Affinity Capture-MS Homo sapiens
32 CHTOP  
Affinity Capture-MS Homo sapiens
33 MECP2 4204
Affinity Capture-MS Homo sapiens
34 MYCN  
Affinity Capture-MS Homo sapiens
35 THOC1 9984
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
36 KIF14 9928
Affinity Capture-MS Homo sapiens
37 KLF16  
Affinity Capture-MS Homo sapiens
38 E2F4  
Affinity Capture-MS Homo sapiens
39 MYO1G 64005
Affinity Capture-MS Homo sapiens
40 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 PPIL1 51645
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 RPS10 6204
Co-fractionation Homo sapiens
44 RRP9 9136
Co-fractionation Homo sapiens
45 C17orf85  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 C19orf47  
Affinity Capture-MS Homo sapiens
47 OXTR 5021
Two-hybrid Homo sapiens
48 Vav2  
Affinity Capture-MS Mus musculus
49 ZPR1 8882
Co-fractionation Homo sapiens
50 KIAA1522 57648
Affinity Capture-MS Homo sapiens
51 HIST1H1C 3006
Co-fractionation Homo sapiens
52 RPL27A 6157
Affinity Capture-MS Homo sapiens
53 RFC4 5984
Co-fractionation Homo sapiens
54 DDX39B 7919
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
55 ZC3H15 55854
Co-fractionation Homo sapiens
56 ZKSCAN5 23660
Co-fractionation Homo sapiens
57 BCLAF1 9774
Affinity Capture-MS Homo sapiens
58 RBM39 9584
Affinity Capture-MS Homo sapiens
59 FANCD2  
Affinity Capture-MS Homo sapiens
60 SNRPB 6628
Affinity Capture-MS Homo sapiens
61 PDGFRA 5156
Affinity Capture-MS Homo sapiens
62 TJP1 7082
Co-fractionation Homo sapiens
63 UBR7  
Co-fractionation Homo sapiens
64 CSNK2A1 1457
Biochemical Activity Homo sapiens
65 ZC3H11A  
Affinity Capture-MS Homo sapiens
66 NXF1 10482
Reconstituted Complex Homo sapiens
67 SF3A1 10291
Affinity Capture-MS Homo sapiens
68 XPO4 64328
Co-fractionation Homo sapiens
69 TJP2 9414
Co-fractionation Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 LSM3 27258
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 TOP1 7150
Affinity Capture-MS Homo sapiens
73 RPL22 6146
Affinity Capture-MS Homo sapiens
74 RNF2  
Affinity Capture-MS Homo sapiens
75 FYTTD1  
Affinity Capture-MS Homo sapiens
76 RBM33  
Affinity Capture-MS Homo sapiens
77 PSMB1 5689
Co-fractionation Homo sapiens
78 Ccdc9  
Affinity Capture-MS Mus musculus
79 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
80 CPSF6 11052
Affinity Capture-MS Homo sapiens
81 RPL6 6128
Affinity Capture-MS Homo sapiens
82 THOC3  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
83 SSRP1 6749
Affinity Capture-MS Homo sapiens
84 SNRPA 6626
Affinity Capture-MS Homo sapiens
85 SMAD4  
Affinity Capture-RNA Homo sapiens
86 HSPB1 3315
Two-hybrid Homo sapiens
87 EIF4A3 9775
Affinity Capture-MS Homo sapiens
88 SIRT1  
Affinity Capture-MS Homo sapiens
89 MKI67  
Affinity Capture-MS Homo sapiens
90 DDX39A 10212
Affinity Capture-MS Homo sapiens
91 Arhgap8  
Affinity Capture-MS Mus musculus
92 Srsf1 110809
Affinity Capture-MS Mus musculus
93 ALYREF 10189
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
94 SERPINB12 89777
Affinity Capture-MS Homo sapiens
95 DNAJC17  
Proximity Label-MS Homo sapiens
96 KIF1C 10749
Affinity Capture-MS Homo sapiens
97 VIM 7431
Co-fractionation Homo sapiens
98 NEMF 9147
Affinity Capture-MS Homo sapiens
99 ZNF830  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 IMP4  
Affinity Capture-MS Homo sapiens
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