Gene description for ALYREF
Gene name Aly/REF export factor
Gene symbol ALYREF
Other names/aliases ALY
ALY/REF
BEF
REF
THOC4
Species Homo sapiens
 Database cross references - ALYREF
ExoCarta ExoCarta_10189
Entrez Gene 10189
HGNC 19071
MIM 604171
UniProt Q86V81  
 ALYREF identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 19056867    
 Gene ontology annotations for ALYREF
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mRNA 3'-end processing GO:0031124 TAS
    regulation of DNA recombination GO:0000018 IMP
    transcription from RNA polymerase II promoter GO:0006366 TAS
    gene expression GO:0010467 TAS
    replication fork processing GO:0031297 IMP
    termination of RNA polymerase II transcription GO:0006369 TAS
    RNA splicing GO:0008380 TAS
    positive regulation of DNA-templated transcription, elongation GO:0032786 IMP
    osteoblast differentiation GO:0001649 IDA
    viral mRNA export from host cell nucleus GO:0046784 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    mRNA export from nucleus GO:0006406 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IEA
    nucleus GO:0005634 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    transcription export complex GO:0000346 IDA
    exon-exon junction complex GO:0035145 IDA
    extracellular exosome GO:0070062 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified ALYREF in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ALYREF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THOC2 57187
Invitro Homo sapiens
2 DDX39A 10212
Reconstituted Complex Homo sapiens
3 RUNX2  
Reconstituted Complex Homo sapiens
4 BAT1  
Reconstituted Complex Homo sapiens
5 LEF1  
Reconstituted Complex Homo sapiens
6 THOC1 9984
Invitro Homo sapiens
7 ALYREF 10189
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 RBM8A 9939
Affinity Capture-Western Homo sapiens
9 THOC3  
Invitro Homo sapiens
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