Experiment description of studies that identified NCL in exosomes

1 Experiment ID 79 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD81|MHCII EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

2 Experiment ID 81 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD81|MHCII EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

3 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 217 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD81|CD9|CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for NCL

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SP1   Affinity Capture-Western Homo sapiens 2 TERT   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 3 HTATSF1 27336 Invivo Homo sapiens Invitro Homo sapiens 4 TP53   Invivo Homo sapiens Invitro Homo sapiens Affinity Capture-MS Homo sapiens 5 CENTA1   Invivo Homo sapiens Invitro Homo sapiens Affinity Capture-MS Homo sapiens 6 NPM1 4869 Invivo Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 7 CYFIP1 23191 Affinity Capture-Western Homo sapiens 8 PRKCZ 5590 Biochemical Activity Homo sapiens Affinity Capture-MS Homo sapiens 9 MYB   Affinity Capture-Western Homo sapiens 10 PPP1CB 5500 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 11 MDK 4192 Invitro Homo sapiens Affinity Capture-MS Homo sapiens 12 S100A11 6282 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 13 PPARGC1A   Invivo Homo sapiens 14 SSB 6741 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 15 SWAP70 23075 Co-purification Homo sapiens Affinity Capture-MS Homo sapiens 16 CD3E 916 Reconstituted Complex Homo sapiens 17 TOP1 7150 Invivo Homo sapiens Invivo Homo sapiens Invitro Homo sapiens Invitro Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 18 CSNK2A2 1459 Affinity Capture-Western Homo sapiens Biochemical Activity Homo sapiens Far Western Homo sapiens 19 NR3C1 2908 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 20 GZMA 3001 Affinity Capture-MS Homo sapiens 21 MYBL1   Affinity Capture-Western Homo sapiens Far Western Homo sapiens View the network

Pathways in which NCL is involved

No pathways found