Gene description for RFC3
Gene name replication factor C (activator 1) 3, 38kDa
Gene symbol RFC3
Other names/aliases RFC38
Species Homo sapiens
 Database cross references - RFC3
ExoCarta ExoCarta_5983
Entrez Gene 5983
HGNC 9971
MIM 600405
UniProt P40938  
 RFC3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RFC3
Molecular Function
    DNA clamp loader activity GO:0003689 TAS
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IDA
    ATPase activity GO:0016887 IDA
Biological Process
    DNA damage response, detection of DNA damage GO:0042769 TAS
    error-prone translesion synthesis GO:0042276 TAS
    DNA-dependent DNA replication GO:0006261 IBA
    error-free translesion synthesis GO:0070987 TAS
    response to organophosphorus GO:0046683 IEP
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    DNA replication GO:0006260 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    translesion synthesis GO:0019985 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    mitotic cell cycle GO:0000278 TAS
    base-excision repair GO:0006284 TAS
    DNA synthesis involved in DNA repair GO:0000731 TAS
    DNA repair GO:0006281 TAS
    telomere maintenance via recombination GO:0000722 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    nucleotide-excision repair GO:0006289 TAS
    telomere maintenance GO:0000723 TAS
Subcellular Localization
    DNA replication factor C complex GO:0005663 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RFC3 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RFC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RFC1 5981
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 PCNA 5111
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
3 CHTF18 63922
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 RFC4 5984
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
5 RAD17  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
6 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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