Gene description for PURB
Gene name purine-rich element binding protein B
Gene symbol PURB
Other names/aliases PURBETA
Species Homo sapiens
 Database cross references - PURB
ExoCarta ExoCarta_5814
Entrez Gene 5814
HGNC 9702
MIM 608887
UniProt Q96QR8  
 PURB identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for PURB
Molecular Function
    single-stranded DNA binding GO:0003697 ISS
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    double-stranded DNA binding GO:0003690 IEA
    poly(A) RNA binding GO:0044822 IDA
    SMAD binding GO:0046332 IEA
    transcription factor binding GO:0008134 ISS
    mRNA binding GO:0003729 ISS
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    cell proliferation GO:0008283 IEA
    regulation of myeloid cell differentiation GO:0045637 NAS
    cell differentiation GO:0030154 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    DNA replication factor A complex GO:0005662 ISS
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified PURB in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PURB
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PURB is involved
No pathways found





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