Gene description for CNOT7
Gene name CCR4-NOT transcription complex, subunit 7
Gene symbol CNOT7
Other names/aliases CAF1
Caf1a
hCAF-1
Species Homo sapiens
 Database cross references - CNOT7
ExoCarta ExoCarta_29883
Vesiclepedia VP_29883
Entrez Gene 29883
HGNC 14101
MIM 604913
UniProt Q9UIV1  
 CNOT7 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CNOT7
Molecular Function
    3'-5'-RNA exonuclease activity GO:0000175 IDA
    transcription corepressor activity GO:0003714 IMP
    RNA exonuclease activity GO:0004532 IDA
    poly(A)-specific ribonuclease activity GO:0004535 IBA
    poly(A)-specific ribonuclease activity GO:0004535 IDA
    protein binding GO:0005515 IPI
    piRNA binding GO:0034584 IEA
    metal ion binding GO:0046872 IEA
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 IBA
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 IDA
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 NAS
    deadenylation-dependent decapping of nuclear-transcribed mRNA GO:0000290 IDA
    regulation of translation GO:0006417 IEA
    positive regulation of cell population proliferation GO:0008284 IMP
    negative regulation of cell population proliferation GO:0008285 IDA
    negative regulation of gene expression GO:0010629 IMP
    regulatory ncRNA-mediated gene silencing GO:0031047 ISS
    P-body assembly GO:0033962 IEA
    miRNA-mediated gene silencing by mRNA destabilization GO:0035279 IDA
    regulation of tyrosine phosphorylation of STAT protein GO:0042509 IMP
    positive regulation of viral genome replication GO:0045070 IMP
    negative regulation of DNA-templated transcription GO:0045892 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    defense response to virus GO:0051607 IMP
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 IMP
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IMP
    positive regulation of mRNA catabolic process GO:0061014 IMP
    piRNA-mediated gene silencing by mRNA destabilization GO:0140991 IEA
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IMP
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 ISS
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IMP
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    nuclear body GO:0016604 IDA
    nuclear speck GO:0016607 IMP
    CCR4-NOT complex GO:0030014 IDA
    CCR4-NOT complex GO:0030014 NAS
    CCR4-NOT core complex GO:0030015 IBA
    intracellular membraneless organelle GO:0043232 IDA
 Experiment description of studies that identified CNOT7 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CNOT7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C9orf78 51759
Affinity Capture-MS Homo sapiens
2 CDK1 983
Affinity Capture-Western Homo sapiens
3 DDX6 1656
Co-fractionation Homo sapiens
4 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
5 TCP11L1 55346
Two-hybrid Homo sapiens
6 BTG1 694
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 TEX13A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MMP7 4316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RNF219  
Reconstituted Complex Homo sapiens
11 TOB2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RAVER1 125950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ZFP36  
Affinity Capture-Western Homo sapiens
14 C12orf57 113246
Affinity Capture-MS Homo sapiens
15 CDK2 1017
Affinity Capture-Western Homo sapiens
16 NMI  
Two-hybrid Homo sapiens
17 XPO1 7514
Affinity Capture-MS Homo sapiens
18 AGO1  
Affinity Capture-MS Homo sapiens
19 RC3H2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
20 AGO2 27161
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 EPAS1  
Affinity Capture-MS Homo sapiens
22 AURKA 6790
Affinity Capture-MS Homo sapiens
23 LSM3 27258
Two-hybrid Homo sapiens
24 RQCD1 9125
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 IKBKG 8517
Reconstituted Complex Homo sapiens
26 BTG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PABPN1L  
Affinity Capture-MS Homo sapiens
28 TSG101 7251
Two-hybrid Homo sapiens
29 GADD45B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CNOT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 FHL3 2275
Two-hybrid Homo sapiens
33 MRFAP1L1  
Two-hybrid Homo sapiens
34 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
35 BTG2 7832
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
36 FPR1  
Affinity Capture-MS Homo sapiens
37 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CNOT3 4849
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MAGEA9  
Affinity Capture-MS Homo sapiens
40 TNRC6B  
Affinity Capture-Western Homo sapiens
41 MEX3C 51320
Affinity Capture-Western Homo sapiens
42 CPEB1  
Proximity Label-MS Homo sapiens
43 PSMC1 5700
Two-hybrid Homo sapiens
44 HOXD4  
Two-hybrid Homo sapiens
45 TNKS1BP1 85456
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 Cnot3 232791
Affinity Capture-MS Mus musculus
47 CNOT10 25904
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 NFKB1 4790
Affinity Capture-MS Homo sapiens
49 TNRC6C  
Affinity Capture-Western Homo sapiens
50 FANCD2OS  
Affinity Capture-MS Homo sapiens
51 CNOT11 55571
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PABPC1 26986
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 NANOS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PIAS1 8554
Two-hybrid Homo sapiens
55 CNOT1 23019
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SCARA3  
Two-hybrid Homo sapiens
57 CNOT8  
Negative Genetic Homo sapiens
58 DYRK1A 1859
Affinity Capture-MS Homo sapiens
59 MINK1 50488
Affinity Capture-MS Homo sapiens
60 APEH 327
Co-fractionation Homo sapiens
61 SCGN  
Affinity Capture-MS Homo sapiens
62 RIBC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 FOXC2  
Two-hybrid Homo sapiens
64 Cnot2  
Affinity Capture-MS Mus musculus
65 CNOT6L 246175
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SH3GLB2 56904
Two-hybrid Homo sapiens
67 FHL2 2274
Affinity Capture-MS Homo sapiens
68 GPBP1L1  
Affinity Capture-MS Homo sapiens
69 Rpl35 66489
Affinity Capture-MS Mus musculus
70 TOB1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TNRC6A 27327
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 TEX11  
Two-hybrid Homo sapiens
73 PURB 5814
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 RAD54L2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PSMC2 5701
Two-hybrid Homo sapiens
76 TMEM131  
Affinity Capture-MS Homo sapiens
77 Zfp36  
Reconstituted Complex Mus musculus
78 NANOS1 340719
Affinity Capture-MS Homo sapiens
79 MKI67  
Affinity Capture-MS Homo sapiens
80 APP 351
Reconstituted Complex Homo sapiens
81 CNOT6  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
83 CAPZA1 829
Affinity Capture-MS Homo sapiens
84 SULT1C4  
Affinity Capture-MS Homo sapiens
85 CDK4 1019
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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