Gene description for CNOT7
Gene name CCR4-NOT transcription complex, subunit 7
Gene symbol CNOT7
Other names/aliases CAF1
Caf1a
hCAF-1
Species Homo sapiens
 Database cross references - CNOT7
ExoCarta ExoCarta_29883
Vesiclepedia VP_29883
Entrez Gene 29883
HGNC 14101
MIM 604913
UniProt Q9UIV1  
 CNOT7 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CNOT7
Molecular Function
    protein binding GO:0005515 IPI
    poly(A)-specific ribonuclease activity GO:0004535 IDA
    exoribonuclease activity GO:0004532 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    RNA binding GO:0003723 IEA
    3'-5'-exoribonuclease activity GO:0000175 IDA
    metal ion binding GO:0046872 IEA
    signal transducer activity GO:0004871 TAS
Biological Process
    RNA phosphodiester bond hydrolysis, exonucleolytic GO:0090503 IEA
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 IMP
    signal transduction GO:0007165 TAS
    cytoplasmic mRNA processing body assembly GO:0033962 IEA
    negative regulation of gene expression GO:0010629 IMP
    gene silencing by miRNA GO:0035195 TAS
    deadenylation-dependent decapping of nuclear-transcribed mRNA GO:0000290 IEA
    gene silencing by RNA GO:0031047 ISS
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    transcription, DNA-templated GO:0006351 IEA
    negative regulation of cell proliferation GO:0008285 IDA
    gene expression GO:0010467 TAS
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 TAS
    exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay GO:0043928 IDA
    regulation of translation GO:0006417 IEA
    positive regulation of mRNA catabolic process GO:0061014 IMP
    carbohydrate metabolic process GO:0005975 TAS
    positive regulation of cell proliferation GO:0008284 IMP
Subcellular Localization
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    cytoplasmic mRNA processing body GO:0000932 ISS
    nucleus GO:0005634 IDA
    CCR4-NOT complex GO:0030014 NAS
 Experiment description of studies that identified CNOT7 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CNOT7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK1 983
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 TOB1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 PABPC1 26986
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 TOB2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 CNOT6  
Two-hybrid Homo sapiens
7 CDK4 1019
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 BTG2 7832
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 BTG1 694
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
10 CDK6 1021
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CNOT7 is involved
PathwayEvidenceSource
Deadenylation of mRNA TAS Reactome





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