Gene description for RQCD1
Gene name RCD1 required for cell differentiation1 homolog (S. pombe)
Gene symbol RQCD1
Other names/aliases CNOT9
CT129
RCD-1
RCD1
Species Homo sapiens
 Database cross references - RQCD1
ExoCarta ExoCarta_9125
Entrez Gene 9125
HGNC 10445
MIM 612054
UniProt Q92600  
 RQCD1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for RQCD1
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    regulation of translation GO:0006417 IEA
    negative regulation of intracellular estrogen receptor signaling pathway GO:0033147 IMP
    gene expression GO:0010467 TAS
    positive regulation of ligand-dependent nuclear receptor transcription coactivator activity GO:2000327 IDA
    cytokine-mediated signaling pathway GO:0019221 IEA
    gene silencing by RNA GO:0031047 IEA
    sex differentiation GO:0007548 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 TAS
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    cytoplasmic mRNA processing body GO:0000932 ISS
    membrane GO:0016020 IDA
    CCR4-NOT complex GO:0030014 IDA
 Experiment description of studies that identified RQCD1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RQCD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RQCD1 is involved
PathwayEvidenceSource
Deadenylation of mRNA TAS Reactome





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