Gene description for RQCD1
Gene name RCD1 required for cell differentiation1 homolog (S. pombe)
Gene symbol RQCD1
Other names/aliases CNOT9
CT129
RCD-1
RCD1
Species Homo sapiens
 Database cross references - RQCD1
ExoCarta ExoCarta_9125
Vesiclepedia VP_9125
Entrez Gene 9125
HGNC 10445
MIM 612054
UniProt Q92600  
 RQCD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for RQCD1
Molecular Function
    epidermal growth factor receptor binding GO:0005154 IPI
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    protein domain specific binding GO:0019904 IPI
    nuclear receptor coactivator activity GO:0030374 IDA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 NAS
    sex differentiation GO:0007548 TAS
    negative regulation of translation GO:0017148 IBA
    cytokine-mediated signaling pathway GO:0019221 IEA
    regulatory ncRNA-mediated gene silencing GO:0031047 IEA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    negative regulation of intracellular estrogen receptor signaling pathway GO:0033147 IMP
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IMP
    positive regulation of DNA-templated transcription GO:0045893 IEA
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 ISS
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    CCR4-NOT complex GO:0030014 IDA
    CCR4-NOT complex GO:0030014 NAS
    CCR4-NOT core complex GO:0030015 IBA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified RQCD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RQCD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
2 ATP6V1B2 526
Co-fractionation Homo sapiens
3 CNOT7 29883
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
4 MINK1 50488
Affinity Capture-MS Homo sapiens
5 GIGYF2 26058
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 TEX13A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MMP7 4316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RNF219  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 ETV6  
Proximity Label-MS Homo sapiens
11 CNOT6  
Affinity Capture-MS Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 MESDC2 23184
Two-hybrid Homo sapiens
14 RC3H2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RUVBL2 10856
Affinity Capture-MS Homo sapiens
16 CPEB1  
Proximity Label-MS Homo sapiens
17 GADD45G  
Two-hybrid Homo sapiens
18 CNOT11 55571
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GADD45A  
Two-hybrid Homo sapiens
20 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RQCD1 9125
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
22 NUDCD3 23386
Co-fractionation Homo sapiens
23 GRB10 2887
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 GADD45B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CNOT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 roq  
Protein-peptide Drosophila melanogaster
28 CAPZA1 829
Affinity Capture-MS Homo sapiens
29 EGFR 1956
Negative Genetic Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
31 PCDHA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MEPCE 56257
Affinity Capture-MS Homo sapiens
33 Tnks1bp1  
Affinity Capture-MS Mus musculus
34 CNOT3 4849
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
35 MAGEA9  
Affinity Capture-MS Homo sapiens
36 THRSP  
Affinity Capture-MS Homo sapiens
37 WRAP73 49856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 Cnot3 232791
Affinity Capture-MS Mus musculus
39 RBM18  
Affinity Capture-MS Homo sapiens
40 CNOT10 25904
Affinity Capture-MS Homo sapiens
41 PABPN1L  
Affinity Capture-MS Homo sapiens
42 IZUMO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GLI2  
Proximity Label-MS Homo sapiens
45 FANCD2OS  
Affinity Capture-MS Homo sapiens
46 CNOT1 23019
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
47 bam  
Protein-peptide Drosophila melanogaster
48 VCP 7415
Affinity Capture-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 GIGYF1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
51 CDK9 1025
Affinity Capture-MS Homo sapiens
52 SCGN  
Affinity Capture-MS Homo sapiens
53 RIBC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CT55  
Affinity Capture-MS Homo sapiens
55 Cnot2  
Affinity Capture-MS Mus musculus
56 CNOT6L 246175
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
57 FPR1  
Affinity Capture-MS Homo sapiens
58 KRAS 3845
Synthetic Lethality Homo sapiens
59 PPP2R5C 5527
Co-fractionation Homo sapiens
60 GPBP1L1  
Affinity Capture-MS Homo sapiens
61 ETV4  
Proximity Label-MS Homo sapiens
62 NANOS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 TOB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TNRC6A 27327
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
65 VASN 114990
Affinity Capture-MS Homo sapiens
66 AKT1 207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
67 TRIM74  
Affinity Capture-MS Homo sapiens
68 TMEM131  
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 FHL2 2274
Affinity Capture-MS Homo sapiens
71 ATG16L1 55054
Affinity Capture-MS Homo sapiens
72 MYC  
Affinity Capture-MS Homo sapiens
73 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 DYRK1A 1859
Affinity Capture-MS Homo sapiens
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