Gene description for PPP2R5C
Gene name protein phosphatase 2, regulatory subunit B', gamma
Gene symbol PPP2R5C
Other names/aliases B56G
PR61G
Species Homo sapiens
 Database cross references - PPP2R5C
ExoCarta ExoCarta_5527
Vesiclepedia VP_5527
Entrez Gene 5527
HGNC 9311
MIM 601645
UniProt Q13362  
 PPP2R5C identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PPP2R5C
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 IDA
    protein phosphatase regulator activity GO:0019888 NAS
    protein phosphatase activator activity GO:0072542 IBA
Biological Process
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 IDA
    signal transduction GO:0007165 NAS
    negative regulation of cell population proliferation GO:0008285 IDA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    meiotic sister chromatid cohesion GO:0051177 IBA
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IBA
    protein phosphatase type 2A complex GO:0000159 IDA
    protein phosphatase type 2A complex GO:0000159 NAS
    chromosome, centromeric region GO:0000775 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PPP2R5C in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP2R5C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 FASN 2194
Negative Genetic Homo sapiens
5 CBX1 10951
Affinity Capture-MS Homo sapiens
6 PPP2R5E 5529
Co-fractionation Homo sapiens
7 TBC1D3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PAXIP1  
Two-hybrid Homo sapiens
9 UGP2 7360
Co-fractionation Homo sapiens
10 TP53 7157
Co-localization Homo sapiens
11 WNT3A 89780
Affinity Capture-MS Homo sapiens
12 YWHAB 7529
Affinity Capture-MS Homo sapiens
13 C9orf78 51759
Affinity Capture-MS Homo sapiens
14 MAPK1 5594
Biochemical Activity Homo sapiens
15 YWHAE 7531
Affinity Capture-MS Homo sapiens
16 HTT 3064
Co-fractionation Homo sapiens
17 PPP1CA 5499
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 POLD1 5424
Co-fractionation Homo sapiens
19 JMJD6 23210
Co-fractionation Homo sapiens
20 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 PACSIN2 11252
Co-fractionation Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 SGOL1  
Affinity Capture-MS Homo sapiens
24 EGFR 1956
Affinity Capture-MS Homo sapiens
25 CHEK2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 LSM7  
Co-fractionation Homo sapiens
28 IER3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 KIAA1524 57650
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 DDX58 23586
Affinity Capture-RNA Homo sapiens
31 APP 351
Reconstituted Complex Homo sapiens
32 PPP1CC 5501
Two-hybrid Homo sapiens
33 PPP4C 5531
Affinity Capture-MS Homo sapiens
34 FKBP5 2289
Affinity Capture-MS Homo sapiens
35 PSMG3 84262
Co-fractionation Homo sapiens
36 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
37 API5 8539
Co-fractionation Homo sapiens
38 CCNG2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 SWAP70 23075
Co-fractionation Homo sapiens
42 PPP1CB 5500
Two-hybrid Homo sapiens
43 PRR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 YWHAZ 7534
Affinity Capture-MS Homo sapiens
45 PPP2CA 5515
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 COL4A3BP 10087
Co-fractionation Homo sapiens
47 LSM6 11157
Co-fractionation Homo sapiens
48 PTGES3 10728
Affinity Capture-MS Homo sapiens
49 CCNG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CEMIP 57214
Affinity Capture-MS Homo sapiens
51 FAM13A 10144
Affinity Capture-MS Homo sapiens
52 PPP2R5A 5525
Affinity Capture-Western Homo sapiens
53 PPP2R2A 5520
Co-fractionation Homo sapiens
54 RQCD1 9125
Co-fractionation Homo sapiens
55 PPP2CB 5516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MBP 4155
Biochemical Activity Homo sapiens
57 EP300 2033
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
58 FAF1 11124
Co-fractionation Homo sapiens
59 FAM43A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 OGT 8473
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PPP2R5C is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Costimulation by the CD28 family TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
CTNNB1 S33 mutants aren't phosphorylated TAS Reactome
CTNNB1 S37 mutants aren't phosphorylated TAS Reactome
CTNNB1 S45 mutants aren't phosphorylated TAS Reactome
CTNNB1 T41 mutants aren't phosphorylated TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Intracellular signaling by second messengers TAS Reactome
M Phase TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet homeostasis TAS Reactome
Platelet sensitization by LDL TAS Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Degradation TAS Reactome
Regulation of TP53 Expression and Degradation TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by AMER1 mutants TAS Reactome
Signaling by APC mutants TAS Reactome
Signaling by AXIN mutants TAS Reactome
Signaling by CTNNB1 phospho-site mutants TAS Reactome
Signaling by GSK3beta mutants TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Signaling by WNT in cancer TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional Regulation by TP53 TAS Reactome
Truncations of AMER1 destabilize the destruction complex TAS Reactome





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