Gene description for PPP2R5C
Gene name protein phosphatase 2, regulatory subunit B', gamma
Gene symbol PPP2R5C
Other names/aliases B56G
PR61G
Species Homo sapiens
 Database cross references - PPP2R5C
ExoCarta ExoCarta_5527
Entrez Gene 5527
HGNC 9311
MIM 601645
UniProt Q13362  
 PPP2R5C identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PPP2R5C
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase type 2A regulator activity GO:0008601 NAS
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 IDA
    regulation of protein phosphatase type 2A activity GO:0034047 NAS
    negative regulation of cell proliferation GO:0008285 IDA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    signal transduction GO:0007165 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    chromosome, centromeric region GO:0000775 IDA
    protein phosphatase type 2A complex GO:0000159 NAS
 Experiment description of studies that identified PPP2R5C in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP2R5C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AXIN1  
Invivo Homo sapiens
2 PPP2CA 5515
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 PPP2R5A  
Affinity Capture-Western Homo sapiens
4 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
5 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
6 PRKCD 5580
Invitro Homo sapiens
Invivo Homo sapiens
7 CCNG2  
Affinity Capture-Western Homo sapiens
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