Experiment description of studies that identified PPP4C in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 235 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 191 ISEV standards ✘ EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD81|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for PPP4C

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PPP2R5D   Affinity Capture-MS Homo sapiens 2 PPP4R1 9989 Invitro Homo sapiens Invivo Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 3 CCT8 10694 Affinity Capture-MS Homo sapiens 4 CCT3 7203 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 PPP4R2   Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 6 KIAA1387   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 TIPRL 261726 Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens 8 CCT2 10576 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 CCT6A 908 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 REL 5966 Invitro Homo sapiens Invivo Homo sapiens Two-hybrid Homo sapiens 11 TCP1 6950 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 SMN1 6606 Affinity Capture-Western Homo sapiens 13 CCT4 10575 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 RELA   Invivo Homo sapiens Invitro Homo sapiens Affinity Capture-MS Homo sapiens 15 KIAA2010   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 16 CCT7 10574 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 CCT5 22948 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 BAG6 7917 Affinity Capture-MS Homo sapiens 19 GEMIN4 50628 Affinity Capture-Western Homo sapiens 20 DHX38   Affinity Capture-MS Homo sapiens 21 KIAA1622   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 22 IGBP1   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 23 NFKB1 4790 Invivo Homo sapiens Invitro Homo sapiens 24 TMSB4X 7114 Invitro Homo sapiens Two-hybrid Homo sapiens 25 PPP2R1A 5518 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 CCDC6 8030 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 PPP2CB 5516 Affinity Capture-MS Homo sapiens View the network

Pathways in which PPP4C is involved

No pathways found