Gene description for GEMIN4
Gene name gem (nuclear organelle) associated protein 4
Gene symbol GEMIN4
Other names/aliases HC56
HCAP1
HHRF-1
p97
Species Homo sapiens
 Database cross references - GEMIN4
ExoCarta ExoCarta_50628
Entrez Gene 50628
HGNC 15717
MIM 606969
UniProt P57678  
 GEMIN4 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for GEMIN4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    gene expression GO:0010467 TAS
    spliceosomal snRNP assembly GO:0000387 TAS
    ncRNA metabolic process GO:0034660 TAS
    rRNA processing GO:0006364 TAS
Subcellular Localization
    SMN complex GO:0032797 IDA
    Gemini of coiled bodies GO:0097504 IEA
    cytoplasm GO:0005737 TAS
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    nucleolus GO:0005730 IEA
    membrane GO:0016020 IDA
    small nuclear ribonucleoprotein complex GO:0030532 TAS
    extracellular exosome GO:0070062 IDA
    SMN-Sm protein complex GO:0034719 IDA
 Experiment description of studies that identified GEMIN4 in exosomes
1
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for GEMIN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NFKBIL1  
Affinity Capture-MS Homo sapiens
2 SNRPB 6628
Affinity Capture-MS Homo sapiens
3 BRF2  
Affinity Capture-MS Homo sapiens
4 GEMIN5 25929
Affinity Capture-Western Homo sapiens
5 LGALS3 3958
Reconstituted Complex Homo sapiens
6 ILK 3611
Affinity Capture-MS Homo sapiens
7 FIBP  
Affinity Capture-MS Homo sapiens
8 PPP4C 5531
Affinity Capture-Western Homo sapiens
9 GEMIN6  
Affinity Capture-Western Homo sapiens
10 GIPC1 10755
Affinity Capture-MS Homo sapiens
11 RNPS1 10921
Affinity Capture-MS Homo sapiens
12 SMN1 6606
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
13 MYC  
Affinity Capture-MS Homo sapiens
14 FBL 2091
Affinity Capture-Western Homo sapiens
15 PPP4R2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 CDK4 1019
Affinity Capture-MS Homo sapiens
17 DDX20  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 GSK3B 2932
Affinity Capture-MS Homo sapiens
19 GAR1 54433
Affinity Capture-Western Homo sapiens
20 LGALS1 3956
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
21 AGO2 27161
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which GEMIN4 is involved
PathwayEvidenceSource
snRNP Assembly TAS Reactome





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