Gene description for WHSC1
Gene name Wolf-Hirschhorn syndrome candidate 1
Gene symbol WHSC1
Other names/aliases MMSET
NSD2
REIIBP
TRX5
WHS
Species Homo sapiens
 Database cross references - WHSC1
ExoCarta ExoCarta_7468
Entrez Gene 7468
HGNC 12766
MIM 602952
UniProt O96028  
 WHSC1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for WHSC1
Molecular Function
    histone-lysine N-methyltransferase activity GO:0018024 IEA
    chromatin binding GO:0003682 IEA
    sequence-specific DNA binding GO:0043565 IEA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
Biological Process
    atrial septum primum morphogenesis GO:0003289 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    bone development GO:0060348 IEA
    regulation of establishment of protein localization GO:0070201 IEA
    histone H3-K36 methylation GO:0010452 IEA
    regulation of double-strand break repair via nonhomologous end joining GO:2001032 IEA
    transcription, DNA-templated GO:0006351 IEA
    membranous septum morphogenesis GO:0003149 IEA
    atrial septum secundum morphogenesis GO:0003290 IEA
    positive regulation of isotype switching to IgA isotypes GO:0048298 IEA
    anatomical structure morphogenesis GO:0009653 TAS
    chromatin organization GO:0006325 TAS
    histone H4-K20 methylation GO:0034770 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
    nucleus GO:0005634 IDA
    chromosome GO:0005694 IEA
 Experiment description of studies that identified WHSC1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WHSC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which WHSC1 is involved
PathwayEvidenceSource
PKMTs methylate histone lysines TAS Reactome





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