Gene description for SRSF6
Gene name serine/arginine-rich splicing factor 6
Gene symbol SRSF6
Other names/aliases B52
HEL-S-91
SFRS6
SRP55
Species Homo sapiens
 Database cross references - SRSF6
ExoCarta ExoCarta_6431
Entrez Gene 6431
HGNC 10788
MIM 601944
UniProt Q13247  
 SRSF6 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SRSF6
Molecular Function
    RNA binding GO:0003723 IDA
    nucleotide binding GO:0000166 IEA
    poly(A) RNA binding GO:0044822 IDA
    pre-mRNA binding GO:0036002 IDA
    protein binding GO:0005515 IPI
Biological Process
    regulation of wound healing GO:0061041 IMP
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    positive regulation of epithelial cell proliferation involved in lung morphogenesis GO:0060501 IDA
    gene expression GO:0010467 TAS
    alternative mRNA splicing, via spliceosome GO:0000380 IDA
    negative regulation of keratinocyte differentiation GO:0045617 IMP
    mRNA splice site selection GO:0006376 IMP
    mRNA 3'-end processing GO:0031124 TAS
    RNA splicing GO:0008380 TAS
    regulation of keratinocyte proliferation GO:0010837 IMP
    mRNA splicing, via spliceosome GO:0000398 TAS
    negative regulation of cell death GO:0060548 IDA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IMP
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA export from nucleus GO:0006406 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
Subcellular Localization
    nuclear speck GO:0016607 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified SRSF6 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRSF6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRSF4 6429
Two-hybrid Homo sapiens
2 YWHAQ 10971
Affinity Capture-MS Homo sapiens
3 CLK1  
Affinity Capture-MS Homo sapiens
4 RNPS1 10921
Two-hybrid Homo sapiens
View the network image/svg+xml



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